Spatial and Temporal Aspects of Exocytosis Studied on the Isolated Plasma Membranes

Milošević, Ira

doi:10.1007/978-1-0716-1044-2_21

Cited by 1 publication

(2 citation statements)

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“…Evanescent excitation significantly limits the photodamage of the specimen which explains the potential of TIRF microscopy to track dynamics of single secretory granules fusing with the plasma membrane, endocytic vesicles retrieving cargo, and single-receptor dynamics at the nanometer scale in living cells [70, 77]. To improve the image resolution, it must be noted that TIRFM has recently been applied to isolated plasma membranes coming from live neuroendocrine cells encoding a fluorescent granular protein to study endocytosis and exocytosis events [78, 79].…”

Section: Total Internal Reflection Fluorescence Microscopymentioning

confidence: 99%

“…and postsynaptic AMPA receptors [67,68] Characterization of the size and distribution of actin and syntaxin clusters [60,64,69] 3D ultrastructure of the actin cytoskeleton [59] Study of structure and dynamics of synaptic vesicles during exocytosis [75,76] Tracking of endocytic vesicles and single-receptor dynamics at the nanometer scale [70,78] Study of endocytosis and exocytosis on isolated plasma membranes [77,79] Monitoring real-time shape of secretory granules during fusion [50] and curvature of clathrincoated pits during endocytosis [82] Simultaneous recording and visualization of action potentials and exocytosis [85] sptPALM, single-particle tracking photoactivated localization microscopy; VGCCs, voltage-gated Ca 2+ channels; FRET, Förster resonance energy; FCS, fluorescence correlation spectroscopy transfer.…”

Section: Atomic Force Microscopymentioning

confidence: 99%

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Advanced Imaging Approaches to Reveal Molecular Mechanisms Governing Neuroendocrine Secretion

et al. 2021

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Identification of the molecular mechanisms governing neuroendocrine secretion and resulting intercellular communication is one of the great challenges of cell biology to better understand organism physiology and neurosecretion disruption-related pathologies such as hypertension, neurodegenerative or metabolic diseases. To visualize molecule distribution and dynamics below the diffraction limit, many imaging approaches have been developed and are still emerging. In this review, we provide an overview of the pioneering studies that use transmission electron microscopy, atomic force microscopy, total internal reflection microscopy and super-resolution microscopy in neuroendocrine cells to visualize molecular mechanisms driving neurosecretion processes, including exocytosis and associated fusion pores, endocytosis and associated recycling vesicles, and protein-protein/protein-lipid interactions. Furthermore, the potential and the challenges of these different advanced imaging approaches for application in the study of neuroendocrine cell biology are discussed, aiming to guide researchers to select the best approach for their specific purpose around the crucial but not yet fully understood neurosecretion process.

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