Spatial and Temporal Distribution of Bean Dwarf Mosaic Geminivirus in<i>Phaseolus vulgaris</i>and<i>Nicotiana benthamiana</i>

Wang, Hong Li; Gilbertson, R. L.; Lucas, William J.

doi:10.1094/phyto-86-1204

Cited by 56 publications

(33 citation statements)

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“…Newly emerged leaves were collected from infected plants 21 and 60 dpi, total genomic DNA was extracted, and DNA gel blot hybridization analysis was conducted with a probe composed of the recombinant plasmids pPA98-1-1, pPA98-2-1, and pPA10-3 labeled with [␣- Immunolocalization experiments were conducted with leaf and petiole tissues collected from symptomatic tomato plants (cultivar Glamour) 4 weeks after agroinoculation with the multimeric PA98-1-1 and PA98-2-1 clones. The antibody used for immunolocalization was raised against the Escherichia coli-expressed CP of the bipartite begomovirus Chino del tomate virus (51). Controls were equivalent tissues from uninfected plants probed with the CP antiserum and from infected plants probed with preimmune antiserum.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of a New World Monopartite Begomovirus Causing Leaf Curl Disease of Tomato in Ecuador and Peru Reveals a New Direction in Geminivirus Evolution

Melgarejo

Kon

Rojas

et al. 2013

J Virol

145

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All characterized whitefly-transmitted geminiviruses (begomoviruses) with origins in the New World (NW) have bipartite genomes composed of a DNA-A and DNA-B component. Recently, an NW begomovirus lacking a DNA-B component was associated with tomato leaf curl disease (ToLCD) in Peru, and it was named Tomato leaf deformation virus (ToLDeV). Here, we show that isolates of ToLDeV associated with ToLCD in Ecuador and Peru have a single, genetically diverse genomic DNA that is most closely related to DNA-A components of NW bipartite begomoviruses. Agroinoculation of multimeric clones of the genomic DNA of three ToLDeV genotypes (two variants and a strain) resulted in the development of tomato leaf curl symptoms indistinguishable from those of ToLCD in Ecuador and Peru. Biological properties of these ToLDeV genotypes were similar to those of Old World (OW) monopartite tomato-infecting begomoviruses, including lack of sap transmissibility, phloem limitation, a resistance phenotype in tomato germplasm with the Ty-1 gene, and functional properties of the V1 (capsid protein) and C4 genes. Differences in symptom phenotypes induced by the ToLDeV genotypes in tomato and Nicotiana benthamiana plants were associated with a highly divergent left intergenic region and C4 gene. Together, these results establish that ToLDeV is an emergent NW monopartite begomovirus that is causing ToLCD in Ecuador and Peru. This is the first report of an indigenous NW monopartite begomovirus, and evidence is presented that it emerged from the DNA-A component of a NW bipartite progenitor via convergent evolution and recombination.

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Section: Methodsmentioning

confidence: 99%

“…During this process, DNA-A-containing virions are delivered, via the phloem, to replication-competent cells in the protophloem (51). Here, DNA-A infections are initiated, either in the absence of the DNA-B component or possibly following the loss of this component.…”

Section: Tolcd In Ecuador Andmentioning

confidence: 99%

Characterization of a New World Monopartite Begomovirus Causing Leaf Curl Disease of Tomato in Ecuador and Peru Reveals a New Direction in Geminivirus Evolution

Melgarejo

Kon

Rojas

et al. 2013

J Virol

145

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“…Escape from the phloem to other leaf tissues has been reported for some dicotyledonous host-infecting begomoviruses, such as African cassava mosaic virus and Tomato golden mosaic virus in N. benthamiana (55), Bean dwarf mosaic virus in N. benthamiana and bean plants (52,53), Bean golden mosaic virus if coinfected with Tobacco mosaic virus (2), and mastreviruses which infect monocotyledonous hosts (25).…”

Section: Vol 78 2004 Tylcv and Tylcsv In Single Nuclei 10719mentioning

confidence: 98%

Tête à Tête of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in Single Nuclei

Morilla

Krenz

Jeske

et al. 2004

J Virol

105

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Since 1997 two distinct geminivirus species, Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato yellow leaf curl virus (TYLCV), have caused a similar yellow leaf curl disease in tomato, coexisted in the fields of southern Spain, and very frequently doubly infected single plants. Tomatoes as well as experimental test plants (e.g., Nicotiana benthamiana) showed enhanced symptoms upon mixed infections under greenhouse conditions. Viral DNA accumulated to a similar extent in singly and doubly infected plants. In situ tissue hybridization showed TYLCSV and TYLCV DNAs to be confined to the phloem in both hosts, irrespective of whether they were inoculated individually or in combination. The number of infected nuclei in singly or doubly infected plants was determined by in situ hybridization of purified nuclei. The percentage of nuclei containing viral DNA (i.e., 1.4% in tomato or 6% in N. benthamiana) was the same in plants infected with either TYLCSV, TYLCV, or both. In situ hybridization of doubly infected plants, with probes that discriminate between both DNAs, revealed that at least one-fifth of infected nuclei harbored DNAs from both virus species. Such a high number of coinfected nuclei may explain why recombination between different geminivirus DNAs occurs frequently. The impact of these findings for epidemiology and for resistance breeding concerning tomato yellow leaf curl diseases is discussed.

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“…The traffic of 3a MP:GFP between SE and CC and then out of the SE-CC complex into surrounding tissues suggests that 3a MP also is involved in viral exit out of the SE-CC complex. Some viruses are restricted to the phloem (Sanger et al, 1994;Wang et al, 1996;Ghoshroy et al, 1998), whereas others cannot enter the phloem (Ding et al, 1998;Wang et al, 1998). It will be revealing to test MPs from these viruses for their ability to traffic through PD at the boundary between the SE-CC complex and neighboring cells.…”

Section: Traffic Of An Mp Into and Out Of The Symplasmically Isolatedmentioning

confidence: 99%

Plasmodesma-Mediated Selective Protein Traffic between “Symplasmically Isolated” Cells Probed by a Viral Movement Protein

Itaya¹,

Ma²,

Qi³

et al. 2002

Plant Cell

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Intercellular communication is essential for differentiation and development. In plants, plasmodesmata (PD) form cytoplasmic channels for direct communication. During plant development, programmed reduction in PD number and transport capacity creates the so-called symplasmic domains. Small fluorescent dyes and ions can diffuse among cells within a domain but not across domain boundaries. Such symplasmic isolation is thought to allow groups of cells to differentiate and develop into tissues with distinct structures and functions. Whether or how "symplasmically isolated" cells communicate with one another is poorly understood. One well-documented symplasmic domain is the sieve element-companion cell (SE-CC) complex in the phloem tissue. We report here that, when produced in the CC of transgenic tobacco, the 3a movement protein (3a MP) of Cucumber mosaic virus fused to green fluorescent protein (GFP) can traffic out of the SE-CC complex via PD. The extent of 3a MP:GFP traffic across the boundary between vascular and nonvascular tissues depends on organ type and developmental stage. Our findings provide experimental evidence that endogenous machinery exists for protein traffic between the symplasmically isolated SE-CC complex and neighboring cells. We suggest that PD-mediated traffic of selected macromolecules can be a mechanism for symplasmically isolated cells to communicate with one another.

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Spatial and Temporal Distribution of Bean Dwarf Mosaic Geminivirus inPhaseolus vulgarisandNicotiana benthamiana

Cited by 56 publications

References 0 publications

Characterization of a New World Monopartite Begomovirus Causing Leaf Curl Disease of Tomato in Ecuador and Peru Reveals a New Direction in Geminivirus Evolution

Characterization of a New World Monopartite Begomovirus Causing Leaf Curl Disease of Tomato in Ecuador and Peru Reveals a New Direction in Geminivirus Evolution

Tête à Tête of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in Single Nuclei

Plasmodesma-Mediated Selective Protein Traffic between “Symplasmically Isolated” Cells Probed by a Viral Movement Protein

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