Spatial and Temporal Pathway for Assembly and Constriction of the Contractile Ring in Fission Yeast Cytokinesis

Wu, Jian-Qiu; Kuhn, Jeffrey R.; Kovar, David R.; Pollard, Thomas D.

doi:10.1016/s1534-5807(03)00324-1

Cited by 384 publications

(648 citation statements)

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“…Once compacted, the contractile ring still needs to mature by the addition of several new components such as the unconventional myosin II Myp2 [Bezanilla et al, 1997;Wu et al, 2003], the F-BAR protein Imp2 and paxillin, a modulator of Rho1 [Ge and Balasubramanian, 2008;Pinar et al, 2008] which together with the C2-domain protein Fic1 confers structural integrity to the contractile ring, preventing its fragmentation when it constricts [Roberts-Galbraith et al, 2009]. Constriction requires an active SIN pathway and is finely tuned by phosphorylation of myosin II heavy and light chains [Loo and Balasubramanian, 2008;Sladewski et al, 2009].…”

Section: Maturation and Constriction Of The Contractile Ringmentioning

confidence: 99%

“…Mid1 is the critical protein necessary for the recruitment and early scaffolding of these components [Wu et al, 2003;Celton-Morizur et al, 2004;Motegi et al, 2004;Wu et al, 2006;Coffman et al, 2009;Almonacid et al, 2011;Laporte et al, 2011;Padmanabhan et al, 2011].…”

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

“…Other major proteins recruited to the cytokinetic nodes besides the IQGAP protein Rng2 and myosin II are the F-BAR protein Cdc15 and the formin Cdc12 [Wu et al, 2003;Wu et al, 2006;Coffman et al, 2009;Laporte et al, 2011]. Careful analysis of the localization of contractile ring components in a variety of mutants has led to a model in which Mid1 organizes two independent modules.…”

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

“…This event is triggered by the Plo1-dependent phosphorylation of Mid1 N-terminal fragment which can bind Rng2 directly in vitro [Almonacid et al, 2011]. Noteworthy, the phosphorylation by Plo1 of the same set of amino acids within Mid1 1-100 fragment thus controls at the same time Mid1 activation and Mid1 export from the nucleus, directly linking these two events.Other major proteins recruited to the cytokinetic nodes besides the IQGAP protein Rng2 and myosin II are the F-BAR protein Cdc15 and the formin Cdc12 [Wu et al, 2003;Wu et al, 2006;Coffman et al, 2009;Laporte et al, 2011]. Careful analysis of the localization of contractile ring components in a variety of mutants has led to a model in which Mid1 organizes two independent modules.…”

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Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Rincón

Paoletti

2012

Cytoskeleton

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Cell division is a critical and irreversible step in the cell cycle. The strategies that cells follow to regulate the position of the division plane must take into account the global geometry of the cell as well as position of the genetic material to ensure its accurate segregation into daughter cells of a given cell shape and size. Along the years, research on Schizosaccharomyces pombe, a wellrecognized model organism for cell division studies has allowed a detailed molecular understanding of the spatial mechanisms regulating cytokinesis. Division plane position in this unicellular rod-shaped organism, which divides by the assembly and constriction of a medially placed actomyosin ring, largely depends on the anillinlike protein Mid1. Therefore, the major pathways controlling the position of the division plane converge on Mid1. In this review, we make an overview of the studies that have deciphered how Mid1 localization and scaffolding activities are controlled over the cell cycle to ensure the symmetrical division of fission yeast cells. These studies have revealed new mechanisms generating spatial information based on nuclear shuttling of the division plane factor Mid1 and on the establishment of cortical inhibitory gradients of the cell polarity kinase Pom1. V C 2012 Wiley Periodicals, Inc Key Words: cytokinesis, fission yeast, contractile ring, Mid1, cell division Introduction C ytokinesis is the final step of cell division that physically separates the daughter cells at the end of the cell cycle. It is a universal process, essential for cell proliferation, for the survival of unicellular organisms or for the development of multicellular organisms, whose major features have been well conserved along evolution. As an irreversible event, cytokinesis is under tight spatial and temporal regulation. Defective cytokinesis can indeed alter cell geometry or size, perturb cellular organization within tissues or prevent the accurate transmission of the genetic material, producing aneuploid cells, which can affect the survival of unicellular species or favor cancer and tumor progression in animal species.Cytokinesis is first strictly coordinated with mitotic progression in order to successfully fulfill chromosome segregation. Checkpoint mechanisms monitoring the function of the mitotic apparatus can halt cells in metaphase, which prevents cytokinesis until actual chromosome segregation in anaphase.Second, spatial regulatory pathways define the position of the division plane according to the position of the nucleus at mitotic entry, or of the mitotic apparatus, perpendicular to the axis of chromosome segregation. This ensures the efficient segregation of the genetic material and allows to control the geometry of the daughter cells in response to morphogenetic programs. Cell division can indeed be symmetric or asymmetric to allow the generation of daughter cells of different sizes. Orientation of the division plane is also modulated to properly position the daughter cells inside embryos or tissues and to permit the asymm...

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Section: Maturation and Constriction Of The Contractile Ringmentioning

confidence: 99%

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

mentioning

confidence: 99%

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Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Rincón

Paoletti

2012

Cytoskeleton

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“…During cytokinesis, the tubular collar of septin filaments is split into two separate 'rings' that seem to serve as gaskets that confine and concentrate between them factors that are involved directly in cytokinesis, such as the exocyst component Sec3, an adaptor protein Spa2 and an isoform of chitin synthase Chs2 [31]. This compartmentalization function appears to be conserved in fission yeast, where a double ring of septin filaments encompasses the contractile apparatus and provides for efficient dissolution of the primary septum [32,33]. It appears that septins might have a similar role in mammalian cells because septins are prominent components of the cleavage furrow and a diffusion barrier within the cleavage furrow has been demonstrated [34].…”

Section: Septin Functionsmentioning

confidence: 99%

Some assembly required: yeast septins provide the instruction manual

Versele

Thorner

2005

Trends in Cell Biology

201

199

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Septins are a family of conserved proteins that form hetero-oligomeric complexes that assemble into filaments. The filaments can be organized into linear arrays, coils, rings and gauzes. They serve as membrane-associated scaffolds and as barriers to demarcate local compartments, especially for the establishment of the septation site for cytokinesis. Studies in budding and fission yeast have revealed many of the protein-protein interactions that govern the formation of multi-septin complexes. GTP binding and phosphorylation direct the polymerization of filaments that is required for septin-collar assembly in budding yeast, whereas a homolog of anillin instructs timely formation of the ring of septin filaments at the medial cortex in fission yeast. These insights should aid understanding of the organization and function of the diverse septin structures in animal cells.

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Reining in cytokinesis with a septin corral

Finger

2004

Bioessays

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Septins are a family of conserved GTP-binding proteins that function in cytokinesis in fungi and animals. In budding yeast, septins form scaffolds for assembly of the actomyosin contractile ring at the cleavage plane, a role that does not appear to be conserved in other organisms. The septins form an hourglass-shaped collar at the mother-bud neck, which splits into two rings flanking the division plane at cytokinesis. A recent study(1) demonstrates that these two septin rings constitute diffusion barriers that create a cytokinetic compartment to retain cortical cytokinetic factors in proximity to the cleavage plane.

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Spatial and Temporal Pathway for Assembly and Constriction of the Contractile Ring in Fission Yeast Cytokinesis

Cited by 384 publications

References 56 publications

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Some assembly required: yeast septins provide the instruction manual

Reining in cytokinesis with a septin corral

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