Spatial composition and turnover of the main molecules in the adult glomerular basement membrane

Smith, David W.; Azadi, Azin; Lee, Chang Joon; Gardiner, Bruce S.

doi:10.1080/21688370.2022.2110798

Cited by 7 publications

(4 citation statements)

References 91 publications

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“…However, we found that silencing of structural molecules such as laminins and collagens resulted in weak defects in the germline. This is likely a function of the short-term silencing and abundance of these proteins that are pre-deposited during development and stable during the window of analysis owing to their slow turnover (49). We found that growth factors and their likely receptors (proteoglycans) mostly function in the distal pachytene region of the germline, aligning with their previously ascribed roles (6,7).…”

Section: Discussionsupporting

confidence: 69%

A matrisome atlas of germ cell development

Amran

Pigatto

Farley

et al. 2023

Preprint

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The extracellular matrix (matrisome) provides chemical and mechanical cues to control the structure and function of cells and tissues. Yet, comprehensive understanding of how matrisome factors individually and collectively control cell and tissue behavior in vivo is lacking. Here, we systematically investigate the function of 443 conserved matrisome-coding genes in controlling germ cell behavior within a complex tissue - the Caenorhabditis elegans germline. Using high-content imaging, 3D reconstruction and cell behavior analysis of >3500 germlines and >7 million germ cells, we identify specific matrisome factors that regulate germline structure, protein distribution, germ cell cycle and fate, apoptosis, and oocyte health. These findings reveal matrisome networks acting autonomously and non-autonomously to coordinate germ cell behavior, providing new avenues to study and manipulate cell fates.

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Section: Discussionsupporting

confidence: 69%

A matrisome atlas of germ cell development

Amran

Pigatto

Farley

et al. 2023

Preprint

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“…However, we found that silencing of substrates of the remodeling proteins resulted in weak germline defects. As mentioned previously, short-term RNAi may have limited impact on the substrates of remodeling proteins due to the abundance of the pre-deposited, stable proteins with slow turnover 73 . We found that growth factors and their likely receptors (proteoglycans) impact the distal to pachytene region of the germ line, aligning with their previously ascribed roles 6,7 .…”

Section: Discussionmentioning

confidence: 99%

The matrisome landscape controlling in vivo germ cell fates

Amran,

Pigatto,

Farley

et al. 2024

Nat Commun

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The developmental fate of cells is regulated by intrinsic factors and the extracellular environment. The extracellular matrix (matrisome) delivers chemical and mechanical cues that can modify cellular development. However, comprehensive understanding of how matrisome factors control cells in vivo is lacking. Here we show that specific matrisome factors act individually and collectively to control germ cell development. Surveying development of undifferentiated germline stem cells through to mature oocytes in the Caenorhabditis elegans germ line enabled holistic functional analysis of 443 conserved matrisome-coding genes. Using high-content imaging, 3D reconstruction, and cell behavior analysis, we identify 321 matrisome genes that impact germ cell development, the majority of which (>80%) are undescribed. Our analysis identifies key matrisome networks acting autonomously and non-autonomously to coordinate germ cell behavior. Further, our results demonstrate that germ cell development requires continual remodeling of the matrisome landscape. Together, this study provides a comprehensive platform for deciphering how extracellular signaling controls cellular development and anticipate this will establish new opportunities for manipulating cell fates.

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“…The gingival epithelium turnover, with emphasis on the JE, is very fast [ 219 ]. Very little detailed information is available on the turnover of its BM in humans, but in the case of the glomerular BM, it seems to be rapid as well [ 220 ]. In marmoset, the remodeling of the gingival lamina propria is inordinately faster when compared to the other connective tissues [ 221 ].…”

Section: The Extracellular Matrix Of the Periodontium And The Role Of...mentioning

confidence: 99%

Matrix Metalloproteinases in the Periodontium—Vital in Tissue Turnover and Unfortunate in Periodontitis

Radzki,

Negri,

Kusiak

et al. 2024

IJMS

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The extracellular matrix (ECM) is a complex non-cellular three-dimensional macromolecular network present within all tissues and organs, forming the foundation on which cells sit, and composed of proteins (such as collagen), glycosaminoglycans, proteoglycans, minerals, and water. The ECM provides a fundamental framework for the cellular constituents of tissue and biochemical support to surrounding cells. The ECM is a highly dynamic structure that is constantly being remodeled. Matrix metalloproteinases (MMPs) are among the most important proteolytic enzymes of the ECM and are capable of degrading all ECM molecules. MMPs play a relevant role in physiological as well as pathological processes; MMPs participate in embryogenesis, morphogenesis, wound healing, and tissue remodeling, and therefore, their impaired activity may result in several problems. MMP activity is also associated with chronic inflammation, tissue breakdown, fibrosis, and cancer invasion and metastasis. The periodontium is a unique anatomical site, composed of a variety of connective tissues, created by the ECM. During periodontitis, a chronic inflammation affecting the periodontium, increased presence and activity of MMPs is observed, resulting in irreversible losses of periodontal tissues. MMP expression and activity may be controlled in various ways, one of which is the inhibition of their activity by an endogenous group of tissue inhibitors of metalloproteinases (TIMPs), as well as reversion-inducing cysteine-rich protein with Kazal motifs (RECK).

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Spatial composition and turnover of the main molecules in the adult glomerular basement membrane

Cited by 7 publications

References 91 publications

A matrisome atlas of germ cell development

A matrisome atlas of germ cell development

The matrisome landscape controlling in vivo germ cell fates

Matrix Metalloproteinases in the Periodontium—Vital in Tissue Turnover and Unfortunate in Periodontitis

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