Spatial confinement-based Figure-of-Eight nanoknots accelerated simultaneous detection and imaging of intracellular microRNAs

Xu, Huo; Zheng, Yanhui; Fang, Xiaojun; Cheng, Yinghao; Xu, Jianguo; Wang, Jie; Li, Hongxia; Lee, Jia; Xue, Chang

doi:10.1016/j.aca.2023.340974

Analytica Chimica Acta

2023

DOI: 10.1016/j.aca.2023.340974

|View full text |Cite

Spatial confinement-based Figure-of-Eight nanoknots accelerated simultaneous detection and imaging of intracellular microRNAs

Huo Xu

Yanhui Zheng

Xiaojun Fang

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2023

2025

Publication Types

Select...

Article6

Relationship

Self Cite0

Independent6

Authors

Journals

Cited by 8 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The construction of nucleic acid probes facilitates the exploration of information in complex biological system. , Particularly, nucleic acid probes can integrate signal amplification strategies that can amplify a single nucleic acid over thousands of times to improve sensitivity. Several enzyme-assisted signal amplification strategies such as loop-mediated isothermal amplification and rolling circle amplification were proposed. − However, these methods are facing the problem of enzyme transportation, which limits their application in living cell imaging. , Alternative enzyme-free amplification strategies, such as hybridization chain reaction (HCR), cascade hybridization reaction (CHA), DNAzyme-based amplification, etc., that can spontaneously occur with the trigger of target nucleic acids have been widely applied in intracellular miRNA imaging. − …”

mentioning

confidence: 99%

DNAzyme-Amplified Cascade Catalytic Hairpin Assembly Nanosystem for Sensitive MicroRNA Imaging in Living Cells

Huang

Tong

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

Sensitive imaging of microRNAs (miRNAs) in living cells is significant for accurate cancer clinical diagnosis and prognosis research studies, but it is challenged by inefficient intracellular delivery, instability of nucleic acid probes, and limited amplification efficiency. Herein, we engineered a DNAzymeamplified cascade catalytic hairpin assembly (CHA)-based nanosystem (DCC) that overcomes these challenges and improves the imaging sensitivity. This enzyme-free amplification nanosystem is based on the sequential activation of DNAzyme amplification and CHA. MnO 2 nanosheets were used as nanocarriers for the delivery of nucleic acid probes, which can resist the degradation by nucleases and supply Mn 2+ for the DNAzyme reaction. After entering into living cells, the MnO 2 nanosheets can be decomposed by intracellular glutathione (GSH) and release the loaded nucleic acid probes. In the presence of target miRNA, the locking strand (L) was hybridized with target miRNA, and the DNAzyme was released, which then cleaved the substrate hairpin (H 1 ). This cleavage reaction resulted in the formation of a trigger sequence (TS) that can activate CHA and recover the fluorescence readout. Meanwhile, the DNAzyme was released from the cleaved H 1 and bound to other H 1 for new rounds of DNAzyme-based amplification. The TS was also released from CHA and involved in the new cycle of CHA. By this DCC nanosystem, low-abundance target miRNA can activate many DNAzyme and generate numerous TS for CHA, resulting in sensitive and selective analysis of miRNAs with a limit of detection of 5.4 pM, which is 18-fold lower than that of the traditional CHA system. This stable, sensitive, and selective nanosystem holds great potential for miRNA analysis, clinical diagnosis, and other related biomedical applications.

show abstract

mentioning

confidence: 99%

DNAzyme-Amplified Cascade Catalytic Hairpin Assembly Nanosystem for Sensitive MicroRNA Imaging in Living Cells

Huang

Tong

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

show abstract

On‐Site Multiply Stimulated Self‐Confinement of an Integrated DNA Cascade Circuit for Highly Reliable Intracellular Imaging of miRNA and In Situ Interrogation of the Relevant Regulatory Pathway

Jiang,

Chen,

Shang

et al. 2024

Small

View full text Add to dashboard Cite

Artificial DNA circuits represent a versatile yet promising toolbox for in situ monitoring and concomitant regulation of diverse biological events within live cells. Nonetheless, their performance is significantly impeded by the diffusion‐dominated slow reaction kinetics and the uncontrollable off‐target activation. Herein, a self‐localized cascade (SLC) circuit is reported for the robust and efficient microRNA (miRNA) analysis in living cells. The SLC circuit consists of the cell‐specific localization module and the analyte‐specific signal amplification module. By integrating the reaction probes of these two modules, the complexity of the system is reduced to realize the responsive co‐localization of circuitry probes and the simultaneous cascade signal amplification. Taking advantage of the specifically activated, self‐localized, and cascade design, the SLC circuit successfully achieves the robust miRNA‐21 (miR‐21) imaging and the accurate cells differentiation. Moreover, the reverse regulation mechanism is successfully explored between messenger RNA (mRNA) and miRNA through the engineered SLC circuit and further elucidates the underlying signaling pathways between them. Therefore, the SLC circuit provides a powerful tool for the sensitive detection of intracellular biomolecules and the study of the corresponding cell regulatory mechanisms.

show abstract

Imaging mRNA in vitro and in vivo with nanofirecracker probes via intramolecular hybridization chain reaction

Xu,

Zheng,

Xie

et al. 2024

Biosensors and Bioelectronics

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Spatial confinement-based Figure-of-Eight nanoknots accelerated simultaneous detection and imaging of intracellular microRNAs

Cited by 8 publications

References 28 publications

DNAzyme-Amplified Cascade Catalytic Hairpin Assembly Nanosystem for Sensitive MicroRNA Imaging in Living Cells

DNAzyme-Amplified Cascade Catalytic Hairpin Assembly Nanosystem for Sensitive MicroRNA Imaging in Living Cells

On‐Site Multiply Stimulated Self‐Confinement of an Integrated DNA Cascade Circuit for Highly Reliable Intracellular Imaging of miRNA and In Situ Interrogation of the Relevant Regulatory Pathway

Imaging mRNA in vitro and in vivo with nanofirecracker probes via intramolecular hybridization chain reaction

Contact Info

Product

Resources

About