Spatial distribution and comparative analysis of <i>Aconitum</i> alkaloids in Fuzi using DESI-MSI and UHPLC-QTOF-MS

Ren, Zhenhui; Zhang, Huixia; Liu, Yang; Chen, Xin; Zhang, Shuai; Chen, Shiqi; Li, Daowen; Li, Cun; Jiang, Haiyang

doi:10.1039/d2an02051c

Cited by 7 publications

(1 citation statement)

References 22 publications

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“…The results revealed that the content of less toxic MDAs and NAs, converted from the more toxic DDAs, increased in all processed products. Additionally, the content of benzoylaconine increased post-processing, indicating enhanced pharmacological activity and reduced toxicity ( Ren et al, 2023 ). Furthermore, processed Fuzi can elevate testosterone, superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) levels while decreasing malondialdehyde levels in serum compared to raw Fuzi, thereby mitigating the reproductive toxicity and genotoxicity of Fuzi.…”

Section: Herbal Processingmentioning

confidence: 99%

A review of Aconiti Lateralis Radix Praeparata (Fuzi) for kidney disease: phytochemistry, toxicology, herbal processing, and pharmacology

Wu,

Qian,

Feng

et al. 2024

Front. Pharmacol.

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BackgroundAconiti Lateralis Radix Praeparata, commonly known as Fuzi in. traditional Chinese medicine (TCM), is widely utilized in clinical practice despite its inherent toxicity. Since ancient times, TCM practitioners have explored various processing techniques to broaden its clinical applications and enhance its safety profile. This review aims to summarize the effects of processing on the chemical composition, toxicity, and pharmacological properties of Fuzi, as well as investigate potential underlying mechanisms.MethodsData on phytochemistry, toxicology, pharmacology, and processing methods of Fuzi were gathered from the literature of electronic databases, including Web of Science, PubMed, and CNKI.ResultsFuzi contains over 100 kinds of chemical compounds, including alkaloids, flavonoids, and polysaccharides, among which alkaloids are the main active compounds. Diester-diterpenoid alkaloids are the main contributors to Fuzi’s toxicity and have side effects on some organs, such as the heart, liver, kidneys, nervous system, and reproductive system. The chemical composition of aconite, particularly its alkaloid content, was changed by hydrolysis or substitution reaction during processing to enhance its efficacy and reduce its toxicity. Salted aconite could enhance the therapeutic efficacy of Fuzi in treating kidney diseases and influence its pharmacokinetics.ConclusionProcessing plays an important role in increasing the efficiency and decreasing toxicity of aconite. Further studies are needed to elucidate the changes of aconite before and after processing and the underlying mechanisms of these changes, thereby providing evidence for the clinical safety of drug use.

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Section: Herbal Processingmentioning

confidence: 99%

A review of Aconiti Lateralis Radix Praeparata (Fuzi) for kidney disease: phytochemistry, toxicology, herbal processing, and pharmacology

Wu,

Qian,

Feng

et al. 2024

Front. Pharmacol.

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Comparative analysis of six aconitine‐type alkaloids in decocting extracts between the different processed Fuzi (Aconiti Lateralis Radix praeparata) and its pharmacokinetic behavior in rats by HPLC‐MS/MS

Feng,

Shang,

Yang

et al. 2024

Biomedical Chromatography

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Aconiti Lateralis Radix praeparata (also known as Fuzi in Chinese) has been extensively used in clinic. However, the toxicity issues limit the therapeutic range of Fuzi. Thus, a rapid and sensitive HPLC‐MS/MS method was developed to simultaneously quantify and compare six active/toxic constituents in decocting extracts from four different processed Fuzi products and in rat plasma after oral administration of its decocting extracts. The selectivity, linearity, sensitivity, precisions, accuracy, matrix effects, extraction recoveries, and stability were validated. The comparative analysis of six alkaloids in decocting extracts between the four kinds of Fuzi products were conducted by the validated HPLC‐MS/MS. Principal component analysis (PCA) and orthogonal partial least‐squares discriminate analysis (OPLS‐DA) were adopted to compare the differences of decocting extracts from the different processed Fuzi products. Besides, selecting Heishunpian (HSP) as the representative of the processed Fuzi products, we investigated the pharmacokinetic behaviors of these major alkaloids after oral administration. The developed HPLC‐MS/MS method to simultaneously analyze these aconitine‐type alkaloids in decocting extracts, and its pharmacokinetic behavior after oral administration may pave a way for quality control of Fuzi and monitoring the safety and rationality of clinical prescriptions.

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Tissue distribution of metabolites in Cordyceps cicadae determined by DESI-MSI analysis

Cao,

Wu,

Zhu

et al. 2024

Anal Bioanal Chem

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Spatial distribution and comparative analysis of Aconitum alkaloids in Fuzi using DESI-MSI and UHPLC-QTOF-MS

Abstract: Spatial distribution of Aconitum alkaloids (AAs) in raw Fuzi and content changes after processing by DESI-MSI and UHPLC-QTOF-MS.

Cited by 7 publications

References 22 publications

A review of Aconiti Lateralis Radix Praeparata (Fuzi) for kidney disease: phytochemistry, toxicology, herbal processing, and pharmacology

A review of Aconiti Lateralis Radix Praeparata (Fuzi) for kidney disease: phytochemistry, toxicology, herbal processing, and pharmacology

Comparative analysis of six aconitine‐type alkaloids in decocting extracts between the different processed Fuzi (Aconiti Lateralis Radix praeparata) and its pharmacokinetic behavior in rats by HPLC‐MS/MS

Tissue distribution of metabolites in Cordyceps cicadae determined by DESI-MSI analysis

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