Spatial Distribution and Temporal Variation of the Rice Silicon Transporter Lsi1

Yamaji, Naoki; Feng, Jian

doi:10.1104/pp.106.093005

Cited by 243 publications

(209 citation statements)

References 38 publications

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“…A baixa capacidade de acumular Si está relacionada à absorção e transporte desse elemento na planta, sendo possivelmente uma consequência da ausência ou pequena presença dos transportadores Lsi1 (do solo para a raiz) e Lsi2 (da raiz para o xilema) nas células das raízes (Nikolic et al, 2007;Yamaji & Ma, 2007;Ma & Yamaji, 2008). A maior presença de Si nas raízes quando comparadas aos demais tecidos e a similaridade dos teores de Si nas raízes entre os tratamentos (Figura 1) sugerem que a atividade dos transportadores é baixa e que o processo de absorção/ transporte ocorre predominantemente por difusão passiva, como constatado em tomateiros (Mitani & Ma, 2005).…”

Section: Discussionunclassified

O alto teor de silício no solo inibe o crescimento radicular de cafeeiros sem afetar as trocas gasosas foliares

Ribeiro

Silva²,

Ramos³

et al. 2011

Rev. Bras. Ciênc. Solo

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RESUMOO benefício do silício (Si) no crescimento de plantas cultivadas tem sido observado em vários trabalhos. O objetivo deste estudo foi avaliar os efeitos de Si no crescimento de mudas de cafeeiro cv. Catuaí Vermelho. O experimento foi conduzido em vasos, e as plantas crescidas em casa de vegetação, sem restrição hídrica. A partição de matéria seca entre raízes, caule e folhas, os teores de nutrientes e Si nos tecidos vegetais e no solo e as trocas gasosas foliares foram avaliados em plantas submetidas a doses de silicato de cálcio correspondentes a 0 (controle), 1,5 e 6 Mg ha -1 . O delineamento experimental foi em blocos casualizados, com três tratamentos e 3, 4 ou 18 repetições, dependendo da variável considerada. Cada parcela experimental era composta por uma planta. Em relação à altura e matéria seca total, as plantas de todos os tratamentos apresentaram desenvolvimento satisfatório, com incrementos diários compatíveis com o esperado em cafeeiros bem nutridos. Os tratamentos com silicato de cálcio causaram aumento nos teores de Ca no solo e na planta e de Si no solo. Considerando que o único nutriente alterado pelos tratamentos foi o Ca e que os teores observados na planta podem ser considerados não prejudiciais, as respostas descritas a seguir são consequência do alto teor de Si no solo. Após 130 dias do início do experimento, as plantas tratadas com 6 Mg ha -1 de silicato de cálcio apresentaram menor acúmulo de matéria seca nas raízes e aumento da relação entre a matéria seca da parte (1) Recebido para publicação em julho de 2010 e aprovado em março de 2011.

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Section: Discussionunclassified

O alto teor de silício no solo inibe o crescimento radicular de cafeeiros sem afetar as trocas gasosas foliares

Ribeiro

Silva²,

Ramos³

et al. 2011

Rev. Bras. Ciênc. Solo

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“…Different organs at different growth stages were sampled, and the immunostaining was carried out using an antibody against GFP (A11122; Molecular Probes) as described previously (Yamaji and Ma, 2007).…”

Section: Immunohistological Stainingmentioning

confidence: 99%

A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

et al. 2013

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ORCID ID: 0000-0003-3411-827X (J.F.M.).Heavy metal-transporting P-type ATPase (HMA) has been implicated in the transport of heavy metals in plants. Here, we report the function and role of an uncharacterized member of HMA, OsHMA5 in rice (Oryza sativa). Knockout of OsHMA5 resulted in a decreased copper (Cu) concentration in the shoots but an increased Cu concentration in the roots at the vegetative stage. At the reproductive stage, the concentration of Cu in the brown rice was significantly lower in the mutants than in the wild-type rice; however, there was no difference in the concentrations of iron, manganese, and zinc between two independent mutants and the wild type. The Cu concentration of xylem sap was lower in the mutants than in the wild-type rice. OsHMA5 was mainly expressed in the roots at the vegetative stage but also in nodes, peduncle, rachis, and husk at the reproductive stage. The expression was up-regulated by excess Cu but not by the deficiency of Cu and other metals, including zinc, iron, and manganese, at the vegetative stage. Analysis of the transgenic rice carrying the OsHMA5 promoter fused with green fluorescent protein revealed that it was localized at the root pericycle cells and xylem region of diffuse vascular bundles in node I, vascular tissues of peduncle, rachis, and husk. Furthermore, immunostaining with an antibody against OsHMA5 revealed that it was localized to the plasma membrane. Expression of OsHMA5 in a Cu transport-defective mutant yeast (Saccharomyces cerevisiae) strain restored the growth. Taken together, OsHMA5 is involved in loading Cu to the xylem of the roots and other organs.

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“…Antibody against HvLsi2 has been described by Mitani et al (2009a). HvLsi6 immunostaining was performed in the roots, leaf sheaths and blades, and node I according to Yamaji and Ma (2007). Immunostaining with HvLsi2 was also performed in node I.…”

Section: Immunohistological Stainingmentioning

confidence: 99%

Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley

et al. 2012

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Silicon (Si) is a beneficial element for plant growth. In barley (Hordeum vulgare), Si uptake by the roots is mainly mediated by a Si channel, Low Silicon1 (HvLsi1), and an efflux transporter, HvLsi2. However, transporters involved in the distribution of Si in the shoots have not been identified. Here, we report the functional characterization of a homolog of HvLsi1, HvLsi6. HvLsi6 showed permeability for Si and localized to the plasma membrane. At the vegetative growth stage, HvLsi6 was expressed in both the roots and shoots. The expression level was unaffected by Si supply. In the roots, HvLsi6 was localized in epidermis and cortex cells of the tips, while in the leaf blades and sheaths, HvLsi6 was only localized at parenchyma cells of vascular bundles. At the reproductive growth stage, high expression of HvLsi6 was also found in the nodes. HvLsi6 in node I was polarly located at the transfer cells surrounding the enlarged vascular bundles toward the numerous xylem vessels. These results suggest that HvLsi6 is involved in Si uptake in the root tips, xylem unloading of Si in leaf blade and sheath, and intervascular transfer of Si in the nodes. Furthermore, HvLsi2 was found to be localized at the parenchyma cell layer adjacent to the transfer cells with opposite polarity of HvLsi6, suggesting that the coupling of HvLsi6 and HvLsi2 is involved in the intervascular transfer of Si at the nodes. Si translocated via the enlarged vascular bundles is unloaded to the transfer cells by HvLsi6, followed by HvLsi2 to reload Si to the diffuse vascular bundles, which are connected to the upper part of the plant, especially the panicles, the ultimate Si sink.

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Spatial Distribution and Temporal Variation of the Rice Silicon Transporter Lsi1

Cited by 243 publications

References 38 publications

O alto teor de silício no solo inibe o crescimento radicular de cafeeiros sem afetar as trocas gasosas foliares

O alto teor de silício no solo inibe o crescimento radicular de cafeeiros sem afetar as trocas gasosas foliares

A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

Functional Characterization of a Silicon Transporter Gene Implicated in Silicon Distribution in Barley

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