Spatial distribution of AT- and GC-rich DNA within interphase cell nuclei of Triatoma infestans Klug

Alvarenga, Elenice Monte; Mondin, Mateus; Martins, James A.; Rodrigues, Vera; Vidal, Benedicto de Campos; Rincones, Johana; Carazzolle, Marcelo Falsarella; Andrade, Larissa Mara de; Mello, Maria Luiza S.

doi:10.1016/j.micron.2011.02.002

Cited by 16 publications

(16 citation statements)

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“…Chromocenters of all species had heterochromatin with GC-rich edges with the central region being AT-rich; however, this was more evident in T. infestans due to the large size of chromocenters ( Figures 2D-2I). Our results differ from those of Alvarenga et al (2011), who detected chromocenters that were AT-rich in the central regions and GC-rich in the edge outside of the chromocenter in Malpighian tubule cells. Our immunolabelling data on methylated chromatin in T. infestans showed a greater methylation in the edges of chromocenters compared with other nuclear regions ( Figures 2J-2L), especially due to the large amount of heterochromatin in T. infestans when compared with other species.…”

Section: Resultscontrasting

confidence: 99%

“…Polyploid cystic cells have been poorly studied, but polyploidy was analysed in a few samples of salivary glands of T. infestans and Panstrongylus megistus (Anhê and Azeredo‐Oliveira, 2008), as well as in the Malpighian tubules of T. infestans (Alvarenga et al, 2011). These studies have focused especially on the distribution, composition and epigenetic effects of the nucleolar bodies and heterochromatin.…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetic characterization reveals differences in nuclear organization of cystic cells among Brazilian species of Triatoma (Heteroptera, Reduviidae)

Bardella

Souza

Martin

et al. 2012

Cell Biology International

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Cytogenetic studies in triatomines have described the occurrence of holokinetic chromosomes, heterochromatin distribution and the location of rDNA (ribosomal DNA) sites, but few aspects of nuclear organization in this group have been discussed. We have focused on ultrastructural and cytogenetic features and differences in cystic cells of seminiferous tubules between five species of Triatoma. Cystic cells showed evidence of polyploidy events and heterochromatic blocks appeared predominantly in the central region of the nuclei. Cytogenetic analyses showed that there was variation in chromocenter number between species, and that the central regions were AT-rich [DAPI+ (4',6-diamidino-2-phenylindole+)], whereas the periphery was CG-rich (CMA+). Another characteristic was the distribution of 45S rDNA, which differed according to the chromosomal location of this sequence. In all we have compared aspects of nuclear organization, polyploidy, heterochromatin, rDNA site distribution and methylation levels, as well as the relationships between five species of Triatoma from a cystic cell perspective.

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Section: Resultscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytogenetic characterization reveals differences in nuclear organization of cystic cells among Brazilian species of Triatoma (Heteroptera, Reduviidae)

Bardella

Souza

Martin

et al. 2012

Cell Biology International

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“…The heterochromatin areas affected by VPA and TSA in mouse somatic cells constitute chromocenters [Cerda et al, ]. A similar effect on heterochromatin unpackaging as promoted by VPA has been observed in another cell system, the polyploid cells of the insect Triatoma infestans (Alvarenga EM—unpublished data), where extensive nuclear compartments contain constitutive heterochromatin [Alvarenga et al, ]. Additionally, TSA has been reported to promote the decondensation of condensed chromosome territories and the reduction of heterochromatin content in lamin A/C‐deficient fibroblasts [Galiová et al, ].…”

Section: Discussionmentioning

confidence: 77%

Changes in Chromatin Structure in NIH 3T3 Cells Induced by Valproic Acid and Trichostatin A

2014

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Valproic acid (VPA) and trichostatin A (TSA) are known histone deacetylase inhibitors (HDACIs) with epigenetic activity that affect chromatin supra-organization, nuclear architecture, and cellular proliferation, particularly in tumor cells. In this study, chromatin remodeling with effects extending to heterochromatic areas was investigated by image analysis in non-transformed NIH 3T3 cells treated for different periods with different doses of VPA and TSA under conditions that indicated no loss of cell viability. Image analysis revealed chromatin decondensation that affected not only euchromatin but also heterochromatin, concomitant with a decreased activity of histone deacetylases and a general increase in histone H3 acetylation. Heterochromatin protein 1-α (HP1-α), identified immunocytochemically, was depleted from the pericentromeric heterochromatin following exposure to both HDACIs. Drastic changes affecting cell proliferation and micronucleation but not alteration in CCND2 expression and in ratios of Bcl-2/Bax expression and cell death occurred following a 48-h exposure of the NIH 3T3 cells particularly in response to higher doses of VPA. Our results demonstrated that even low doses of VPA (0.05 mM) and TSA (10 ng/ml) treatments for 1 h can affect chromatin structure, including that of the heterochromatin areas, in non-transformed cells. HP1-α depletion, probably related to histone demethylation at H3K9me3, in addition to the effect of VPA and TSA on histone H3 acetylation, is induced on NIH 3T3 cells. Despite these facts, alterations in cell proliferation and micronucleation, possibly depending on mitotic spindle defects, require a longer exposure to higher doses of VPA and TSA.

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“…The event of polyploidisation present in all cystic cells has been described for cells of other tissues of the triatomines, such as salivary glands (Anhê & Azeredo-Oliveira 2008;Anhê et al 2014) and Malpighian tubules (Alvarenga et al 2011(Alvarenga et al , 2012. This event is critical to the nutritional role that these cells exert on the cells in meiotic division (Schmidt & Dorn 2004), because it allows certain regions of the genome to be endoreplicated.…”

mentioning

confidence: 93%

Analysis of Metabolic Activity in Cystic Cells ofTriatoma rubrofasciata(Hemiptera: Triatominae) and Its Capacity to Occupy Different Environments

et al. 2016

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Spatial distribution of AT- and GC-rich DNA within interphase cell nuclei of Triatoma infestans Klug

Cited by 16 publications

References 50 publications

Cytogenetic characterization reveals differences in nuclear organization of cystic cells among Brazilian species of Triatoma (Heteroptera, Reduviidae)

Cytogenetic characterization reveals differences in nuclear organization of cystic cells among Brazilian species of Triatoma (Heteroptera, Reduviidae)

Changes in Chromatin Structure in NIH 3T3 Cells Induced by Valproic Acid and Trichostatin A

Analysis of Metabolic Activity in Cystic Cells ofTriatoma rubrofasciata(Hemiptera: Triatominae) and Its Capacity to Occupy Different Environments

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