Spatial Divergence of <i>PHR-PHT1</i> Modules Maintains Phosphorus Homeostasis in Soybean Nodules

Lu, Mingyang; Cheng, Zhiyuan; Zhang, Xiaomei; Huang, Penghui; Fan, Chengming; Yu, Guolong; Chen, Fulu; Xu, Kun; Chen, Qingshan; Miao, Yuchen; Han, Yuzhu; Feng, Xianzhong; Liu, Liangyu; Fu, Yong-Fu

doi:10.1104/pp.19.01209

Cited by 58 publications

(56 citation statements)

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“…Interestingly, another nodule-localized Pi transporter GmPT7 appears to play a role in delivering Pi from an external environment to the nodule fixation zone ( Chen et al, 2019 ). PHR1, the constitutively expressed MYB-domain transcription factor, and its downstream target PHT1 constitute the key regulatory modules for symbiotic N fixation in legumes ( Nussaume et al, 2011 ; Lu et al, 2020 ). Added to its complexity, it has been shown that a single soybean GmPHR has more than one GmPHT1 target, and one GmPHT1 is under the control of multiple GmPHRs ( Lu et al, 2020 ).…”

Section: The Role Of Phosphate Signaling and Transport In Symbiotic Nitrogen Fixationmentioning

confidence: 99%

“…PHR1, the constitutively expressed MYB-domain transcription factor, and its downstream target PHT1 constitute the key regulatory modules for symbiotic N fixation in legumes ( Nussaume et al, 2011 ; Lu et al, 2020 ). Added to its complexity, it has been shown that a single soybean GmPHR has more than one GmPHT1 target, and one GmPHT1 is under the control of multiple GmPHRs ( Lu et al, 2020 ). Therefore, different combinations of Pi transporters and Pi stress response factors form diverse regulatory networks in the N fixation and non-fixation zones to maintain Pi homeostasis in response to both systemic and local Pi availability for efficient symbiotic N fixation in soybean nodules ( Lu et al, 2020 ).…”

Section: The Role Of Phosphate Signaling and Transport In Symbiotic Nitrogen Fixationmentioning

confidence: 99%

“…Added to its complexity, it has been shown that a single soybean GmPHR has more than one GmPHT1 target, and one GmPHT1 is under the control of multiple GmPHRs ( Lu et al, 2020 ). Therefore, different combinations of Pi transporters and Pi stress response factors form diverse regulatory networks in the N fixation and non-fixation zones to maintain Pi homeostasis in response to both systemic and local Pi availability for efficient symbiotic N fixation in soybean nodules ( Lu et al, 2020 ). Eleven members of the PT1 family have been identified in M. truncatula , and most of them were highly expressed under conditions of low Pi ( Cao et al, 2020 ).…”

Section: The Role Of Phosphate Signaling and Transport In Symbiotic Nitrogen Fixationmentioning

confidence: 99%

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Nitrogen and Phosphorus Signaling and Transport During Legume–Rhizobium Symbiosis

Yin

Chen

2021

Front. Plant Sci.

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Nitrogen (N) and phosphorus (P) are the two predominant mineral elements, which are not only essential for plant growth and development in general but also play a key role in symbiotic N fixation in legumes. Legume plants have evolved complex signaling networks to respond to both external and internal levels of these macronutrients to optimize symbiotic N fixation in nodules. Inorganic phosphate (Pi) and nitrate (NO3−) are the two major forms of P and N elements utilized by plants, respectively. Pi starvation and NO3− application both reduce symbiotic N fixation via similar changes in the nodule gene expression and invoke local and long-distance, systemic responses, of which N-compound feedback regulation of rhizobial nitrogenase activity appears to operate under both conditions. Most of the N and P signaling and transport processes have been investigated in model organisms, such as Medicago truncatula, Lotus japonicus, Glycine max, Phaseolus vulgaris, Arabidopsis thaliana, Oryza sativa, etc. We attempted to discuss some of these processes wherever appropriate, to serve as references for a better understanding of the N and P signaling and transport during symbiosis.

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Section: The Role Of Phosphate Signaling and Transport In Symbiotic Nitrogen Fixationmentioning

confidence: 99%

Section: The Role Of Phosphate Signaling and Transport In Symbiotic Nitrogen Fixationmentioning

confidence: 99%

Section: The Role Of Phosphate Signaling and Transport In Symbiotic Nitrogen Fixationmentioning

confidence: 99%

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Nitrogen and Phosphorus Signaling and Transport During Legume–Rhizobium Symbiosis

Yin

Chen

2021

Front. Plant Sci.

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“…For example, GmPT5 and GmPT7 were found to promote nodulation ( Qin et al, 2012 ; Chen et al, 2019 ). GmPT7 also could improve Pi content in nodules ( Lu et al, 2020 ). However, M. truncatula as an important model plant for legume, little is known about the function of PHT1 family genes in symbiosis with rhizobium.…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of the Phosphate Transporter Gene MtPT6 From Medicago truncatula

Cao

Liu

et al. 2021

Front. Plant Sci.

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Phosphorus is one of the essential macronutrients required by plant growth and development, but phosphate resources are finite and diminishing rapidly because of the huge need in global agriculture. In this study, 11 genes were found in the Phosphate Transporter 1 (PHT1) family of Medicago truncatula. Seven genes of the PHT1 family were available by qRT-PCR. Most of them were expressed in roots, and almost all genes were induced by low-phosphate stress in the nodule. The expression of MtPT6 was relatively high in nodules and induced by low-phosphate stress. The fusion expression of MtPT6 promoter-GUS gene in M. truncatula suggested that the expression of MtPT6 was induced in roots and nodules by phosphate starvation. In roots, MtPT6 was mainly expressed in vascular tissue and tips, and it was also expressed in cortex under low-phosphate stress; in nodules, it was mainly expressed in vascular bundles, cortical cells, and fixation zone cells. MtPT6 had a close relationship with other PHT1 family members according to amino acid alignment and phylogenetic analysis. Subcellular localization analysis in tobacco revealed that MtPT6 protein was localized to the plasma membrane. The heterologous expression of MtPT6 in Arabidopsis knockout mutants of pht1.1 and pht1.4 made seedlings more susceptible to arsenate treatment, and the phosphate concentrations in pht1.1 were higher in high phosphate condition by expressing MtPT6. We conclude that MtPT6 is a typical phosphate transporter gene and can promote phosphate acquisition efficiency of plants.

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“…The intercropping of soybean and maize is widely practiced as a promising option in China, due to its outstanding advantages in improvement of nutrient (N and P) use efficiency and yield [35][36][37] . Much work on improvement of P content in nodules under P deficiency today were mainly focused on soybean grown alone [38,13,15] , but little information is available about the P level in nodules altered by intercropping of soybean and maize. In this context, in order to elucidate the interaction of P deficiency−nodular P level−nodulation under soybean/maize intercropping, we conducted experiments under both sufficient and deficient P levels to test the hypothesis that intercropped soybean and maize grown under P-deficient conditions could increase the P level in nodules.…”

Section: Introductionmentioning

confidence: 99%

Increased Nodular P Level Induced by Intercropping Stimulated Nodulation in Soybean Under Phosphorus Deficiency

Qin

Pan

Xiao

et al. 2021

Preprint

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Low P availability is a vital constraint for nodulation and efficient N2 fixation of legume, including soybean. To elucidate the mechanisms involved in nodule adaption to low P availability under legume/cereal intercropping systems, two experiments consisting of three cropping patterns (monocropped soybean, monocropped maize, soybean/maize intercropping) were studied under both sufficient-and deficient-P levels. Our results demonstrated that intercropped soybean with maize showed a higher nodulation and N2 fixation efficiency under low P availability than monocropped soybean as evidenced by improvement in the number, dry weight and nitrogenase activity of nodules. These differences might be attributed to increase in P level in intercropping-induced nodules under low P supply, which was caused by the elevated activities of phytase and acid phosphatases in intercropping-induced nodules. Additionally, the enhanced expression of phytase gene in nodules supplied with deficient P level coincided with an increase in phytase and acid phosphatase activities. Our results revealed a mechanism for how intercropped maize stimulated nodulation and N2 fixation of soybean under P deficient environments, where enhanced synthesis of phytase and acid phosphatases in intercropping-induced nodules, and stimulated nodulation and N2 fixation.

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Spatial Divergence of PHR-PHT1 Modules Maintains Phosphorus Homeostasis in Soybean Nodules

Cited by 58 publications

References 56 publications

Nitrogen and Phosphorus Signaling and Transport During Legume–Rhizobium Symbiosis

Nitrogen and Phosphorus Signaling and Transport During Legume–Rhizobium Symbiosis

Functional Analysis of the Phosphate Transporter Gene MtPT6 From Medicago truncatula

Increased Nodular P Level Induced by Intercropping Stimulated Nodulation in Soybean Under Phosphorus Deficiency

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