Spatial Genetic Heterogeneity in Populations of a Newly Invasive Whitefly in China Revealed by a Nation-Wide Field Survey

Chu, Dong; Pan, Hui; Li, Xian Chun; Guo, Dong; Tao, Yun Li; Liu, Bai Ming; Zhang, Youjun

doi:10.1371/journal.pone.0079997

Cited by 11 publications

(10 citation statements)

References 38 publications

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“…Similar spatiotemporal patterns of genetic diversity have also been observed in other invasive species that rapidly expand their range (Dong et al 2013, Schulte et al 2013, Signorile et al 2014.…”

Section: Discussionsupporting

confidence: 73%

Genetic patterns reveal an old introduction event and dispersal limitations despite rapid distribution expansion

Berggren

Cassel‐Lundhagen

2015

Biol Invasions

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Identifying sources of range expansions after an introduction event and understanding the species dispersal are essential for effective management of invasive species. In a unique study system we investigated the spread and distribution of genetic diversity subsequent to a known colonization event and in the light of the well-known biology of the rapidly expanding Roesel's bush-cricket (Metrioptera roeselii), a species that is non-native in Sweden. Using eight microsatellite markers we analyzed genetic variation in 837 individuals collected at 29 sites across the species total range in central Sweden to verify the species local origin and to determine how the species known dispersal biology affect the population genetic structure throughout its range. We found that unique allelic richness was highest in a site approximately 16 km southwest of the previously suggested site of establishment, pointing towards a site of introduction close to a royal stud farm from where it is known that animals have been imported from Europe. Despite the species rapid expansion, genetic diversity in the core of the distribution was higher than in the populations at the range margin. Bayesian cluster analyses also revealed that genetic structuring was more pronounced in the range margin, indicating the occurrence of dichotomous dispersal behaviour of the species with occasions of rare long distance events. Our study shows that good sampling design and appropriate choice of genetic markers can help to identify species local origin and explain genetic patterns that arise during range expansions.

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Section: Discussionsupporting

confidence: 73%

Genetic patterns reveal an old introduction event and dispersal limitations despite rapid distribution expansion

Berggren

Cassel‐Lundhagen

2015

Biol Invasions

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“…The population genetics of B. tabaci has been studied using multiple molecular marker methods such as microsatellite loci (Chu et al, , ) or RAD sequencing (Elfekih et al, ). For example, the population genetics of B. tabaci using the RAD sequencing method by Elfekih et al () did not attempt to evaluate the minimum sample size, in which the sample sizes ranged from two to nine individuals.…”

Section: Discussionmentioning

confidence: 99%

“…The population genetics of B. tabaci has been studied using multiple molecular marker methods such as microsatellite loci (Chu et al, 2011(Chu et al, , 2013 or RAD sequencing (Elfekih et al, 2018). TA B L E 2 The optimal sample sizes required for different genetic parameters limited to a small number of SNPs (3,000) and limited sampling space.…”

Section: Discussionmentioning

confidence: 99%

Minimum sample sizes for invasion genomics: Empirical investigation in an invasive whitefly

et al. 2019

Ecology and Evolution

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Analysis of population genetics provides insights into the evolutionary processes, among which the sample size choice is per se a crucial issue in the analysis. Genome‐wide high‐throughput techniques based on RADseq have been increasingly used in studies on the population genomics of invasive species. However, there is little information available regarding optimal sample sizes for analyzing population genomics of invasive species. In this study, we first use type IIB endonucleases restriction site‐associated DNA (2b‐RAD) to mine thousands of single nucleotide polymorphisms (SNPs) for native and introduced populations in Q1 clade (SPB and 17JN) and Q2 clade (ISQ and UAS0601) of the whitefly, Bemisia tabaci (Gennadius) MED (also known as B. tabaci biotype Q). Then, we used resampling techniques to create simulated populations with a random subset of individuals and 3,000 SNPs to determine how many individuals should be sampled for accurate estimates of intra‐ and interpopulation genetic diversity. We calculated the intrapopulation genetic diversity parameters (unbiased expected heterozygosity, observed heterozygosity, and the number of effect alleles) and pairwise genetic differentiation FST; finally, an ad hoc statistic, ΔK, was used to determine the optimal value. Our results showed that a sample size greater than four individuals (n ≥ 4) has little impact on estimates of genetic diversity within whitefly populations; moreover, precise estimate of FST can be easily achieved at a very small simple size (n = 3 or 4). Our results will provide in‐depth understanding of the optimization of sampling schemes in population genomics of invasive species.

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“…Extensive studies using molecular markers have provided estimates of the potential invasion routes, and of genetic characteristics of a number of invasive species [ 16 , 17 , 18 ]. For example, analysis of the genetic structure of the western flower thrips, Frankliniella occidentalis (Pergande) has revealed the invasion route and potential source of a secondary introduction in China [ 19 ], which could be useful for avoiding further spread and/or additional introductions of this pest.…”

Section: Introductionmentioning

confidence: 99%

Population Structure of the Greenhouse Whitefly, Trialeurodes vaporariorum (Westwood), an Invasive Species from the Americas, 60 Years after Invading China

Gao

Zhang

et al. 2014

IJMS

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Though the greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae) was introduced into China more than 60 years ago, the genetic diversity and structure of this exotic insect pest and virus vector have not been studied. To investigate the population genetic characteristics of this invasive species and to identify potential invasion routes, the genetic diversity and population structure of 17 collections of T. vaporariorum from nine provinces in China were analyzed using seven microsatellite loci. The results of the analyses indicated that the genetic diversity for the populations examined from the four provinces: Jilin, Ningxia, Guizhou and Qinghai, was lower than the genetic diversity of populations from the five provinces: Yunnan, Shandong, Shanxi, Liaoning, and Gansu. The T. vaporariorum populations analyzed in this study grouped as two distinct genetic clusters based on the analysis using STRUCTURE, whereas, 8 clusters were identified based on the BAPS analysis. Of the 136 genetic distance (Fst) values, 128 (94%) were associated with a significant exact test. There was a significant relationship between Fst and geographical distance. These results demonstrate that populations of T. vaporariorum in China exhibit significant genetic differentiation, indicating the likelihood that multiple introductions of T. vaporariorum into China have occurred. Also, the populations collected from the provinces of Jilin, Ningxia, Guizhou and Qinghai appear to represent secondary introductions originating from other Chinese provinces.

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Spatial Genetic Heterogeneity in Populations of a Newly Invasive Whitefly in China Revealed by a Nation-Wide Field Survey

Cited by 11 publications

References 38 publications

Genetic patterns reveal an old introduction event and dispersal limitations despite rapid distribution expansion

Genetic patterns reveal an old introduction event and dispersal limitations despite rapid distribution expansion

Minimum sample sizes for invasion genomics: Empirical investigation in an invasive whitefly

Population Structure of the Greenhouse Whitefly, Trialeurodes vaporariorum (Westwood), an Invasive Species from the Americas, 60 Years after Invading China

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