For the first time, we present an approach to derive zonal, meridional and vertical wavelengths as well as periods of gravity waves based on only one OH* spectrometer addressing one vibrational-rotational transition. Knowledge of these parameters 15 is a precondition for the calculation of further information such as the wave group velocity vector.OH(3-1) spectrometer measurements allow the analysis of gravity wave periods, but spatial information cannot necessarily be deduced. We use a scanning spectrometer and the harmonic analysis to derive horizontal wavelengths at the mesopause above Oberpfaffenhofen (48.09°N, 11.28°E), Germany for 22 nights in 2015. Based on the approximation of the dispersion relation for gravity waves of low and medium frequency and additional horizontal wind information, we calculate vertical 20 wavelengths afterwards. The mesopause wind measurements nearest to Oberpfaffenhofen are conducted at Collm (51.30°N, 13.02°E), Germany, ca. 380 km northeast of Oberfpaffenhofen by a meteor radar.In order to check our results, vertical temperature profiles of TIMED-SABER (Thermosphere Ionosphere Mesosphere Energetics Dynamics, Sounding of the Atmosphere using Broadband Emission Radiometry) overpasses are analysed with respect to the dominating vertical wavelength. 25Atmos. Meas. Tech. Discuss., https://doi