Spatial localization of monoterpenoid indole alkaloids in Rauvolfia tetraphylla by high resolution mass spectrometry imaging

Lorensen, Marcus Daniel Brandbjerg Bohn; Bjarnholt, Nanna; St-Pierre, Benoit; Courdavault, Vincent; Heinicke, Sarah; O’Connor, Sarah E.; Janfelt, Christian

doi:10.1016/j.phytochem.2023.113620

Cited by 14 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At least two replicates were analyzed for each of the three treatments (control, systemic uptake, and sorption). MS‐imaging data analysis was performed as described by Lorensen et al (2023).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Importance of Dietary Uptake for in Situ Bioaccumulation of Systemic Fungicides Using Gammarus pulex as a Model Organism

Raths

Schnurr

Bundschuh

et al. 2023

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

Bioaccumulation of organic contaminants from contaminated food sources might pose an underestimated risk toward shredding invertebrates. This assumption is substantiated by monitoring studies observing discrepancies of predicted tissue concentrations determined from laboratory‐based experiments compared with measured concentrations of systemic pesticides in gammarids. To elucidate the role of dietary uptake in bioaccumulation, gammarids were exposed to leaf material from trees treated with a systemic fungicide mixture (azoxystrobin, cyprodinil, fluopyram, and tebuconazole), simulating leaves entering surface waters in autumn. Leaf concentrations, spatial distribution, and leaching behavior of fungicides were characterized using liquid chromatography coupled with high‐resolution tandem mass spectrometry (LC‐HRMS/MS) and matrix‐assisted laser desorption ionization‐mass spectrometric imaging. The contribution of leached fungicides and fungicides taken up from feeding was assessed by assembling caged (no access) and uncaged (access to leaves) gammarids. The fungicide dynamics in the test system were analyzed using LC‐HRMS/MS and toxicokinetic modeling. In addition, a summer scenario was simulated where water was the initial source of contamination and leaves contaminated by sorption. The uptake, translocation, and biotransformation of systemic fungicides by trees were compound‐dependent. Internal fungicide concentrations of gammarids with access to leaves were much higher than in caged gammarids of the autumn scenario, but the difference was minimal in the summer scenario. In food choice and dissectioning experiments gammarids did not avoid contaminated leaves and efficiently assimilated contaminants from leaves, indicating the relevance of this exposure pathway in the field. The present study demonstrates the potential impact of dietary uptake on in situ bioaccumulation for shredders in autumn, outside the main application period. The toxicokinetic parameters obtained facilitate modeling of environmental exposure scenarios. The uncovered significance of dietary uptake for detritivores warrants further consideration from scientific as well as regulatory perspectives. Environ Toxicol Chem 2023;00:1–14. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The preparation of leaf cryosections was based on Lorensen et al (2023). Sections were created by cutting 16-µm-thick slices of embedded (2.5% carboxymethyl-cellulose) leaves on a cryomicrotome (−16 °C; Leica CM3050S; Leica Microsystems).…”

Section: Characterization Of the Test Materialsmentioning

confidence: 99%

Importance of Dietary Uptake for in Situ Bioaccumulation of Systemic Fungicides Using Gammarus pulex as a Model Organism

Raths

Schnurr

Bundschuh

et al. 2023

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Like flavonoids, given their important pharmaceutical, bioactive properties, it is not surprising that many MSI studies have targeted the spatial profiles of alkaloids to characterize these metabolic pathways and their regulation. For example, two recent imaging studies using DESI- and/or MALDI-MSI showed heterogeneous spatial patterns of monoterpenoid indole alkaloids (MIAs) within diverse tissues of Rauvolfia tetraphylla ( Mohana Kumara et al , 2019 ; Lorensen et al , 2023a ). MIAs were enriched in the early stages of mature fruit, with several compounds restricted to the exocarp (e.g.…”

Section: Expanding the Spatial Metabolome Coveragementioning

confidence: 99%

“…To produce chemically diverse compounds in specific tissues that use similar initial building blocks, such as trypamine (via tryptophan decarboxylation), inherently requires coordinated metabolic regulation and utilization of diverse biochemical reactions ( Horn, 2021 ). Another study focused on using MSI to address the biosynthesis of reserpine, an anti-hypertension and anti-microbial agent (and biochemically related rescinnamine), by analyzing not only the endproduct reserpine (and rescinnamine) but also the theoretical intermediates ( Lorensen et al , 2023a ). High-resolution MALDI-MSI provided unprecedented detail, detecting most of the proposed intermediates of reserpine in similar cell types among diverse tissues, and this was reinforced using a deuterium-labeled trypamine precursor.…”

Section: Expanding the Spatial Metabolome Coveragementioning

confidence: 99%

Imaging plant metabolism in situ

Horn,

Chapman

2023

Journal of Experimental Botany

View full text Add to dashboard Cite

Mass spectrometry imaging (MSI) has emerged as an invaluable analytical technique for investigating the spatial distribution of molecules within biological systems. In the realm of plant science, MSI is increasingly employed to explore metabolic processes across a wide array of plant tissues, including those in leaves, fruits, stems, roots, and seeds, spanning various plant systems such as model species, staple and energy crops, and medicinal plants. By generating spatial maps of metabolites, MSI has elucidated the distribution patterns of diverse metabolites and phytochemicals, encompassing lipids, carbohydrates, amino acids, organic acids, phenolics, terpenes, alkaloids, vitamins, pigments, and others, thereby providing insights into their metabolic pathways and functional roles. In this review, we present recent MSI studies that demonstrate the advances made in visualizing the plant spatial metabolome. Moreover, we emphasize the technical progresses that enhance the identification and interpretation of spatial metabolite maps. Within a mere decade since the inception of plant MSI studies, this robust technology is poised to continue as a vital tool for tackling complex challenges in plant metabolism.

show abstract

“…Moreover, the advancement of mass spectrometry imaging (MSI) techniques has facilitated the visualization of the spatial distribution of the primary/secondary metabolites in diverse plant tissues with high sensitivity and extensive coverage . Matrix-assisted laser desorption/ionization (MALDI) and desorption electrospray ionization (DESI) are currently two most frequently used ionization techniques in MSI to visualize the dynamic spatial distributions of the functional metabolites. − Compared with MALDI-MSI, DESI-MSI does not require matrix spraying, thus avoiding matrix ion interference. , MSI analysis can provide important information for understanding the distribution and specific biofunctions of metabolites in plants. An atmospheric pressure matrix-assisted laser desorption (AP-MALDI) MSI method could identify and visualize the spatial distribution of free flavonoids, flavonoid glycosides, and saponins of Glycyrrhiza glabra at the cellular level .…”

Section: Introductionmentioning

confidence: 99%

Spatial Distribution and Characterization of the Small-Molecule Metabolites and In Situ Hydrolyzed Oligosaccharides in the Rhizome of Glycyrrhiza uralensis by Desorption Electrospray Ionization-Mass Spectrometry Imaging and High-Resolution Liquid Chromatography–Mass Spectrometry

Zhao,

Jiang,

Liu

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Spatial localization of monoterpenoid indole alkaloids in Rauvolfia tetraphylla by high resolution mass spectrometry imaging

Cited by 14 publications

References 37 publications

Importance of Dietary Uptake for in Situ Bioaccumulation of Systemic Fungicides Using Gammarus pulex as a Model Organism

Importance of Dietary Uptake for in Situ Bioaccumulation of Systemic Fungicides Using Gammarus pulex as a Model Organism

Imaging plant metabolism in situ

Spatial Distribution and Characterization of the Small-Molecule Metabolites and In Situ Hydrolyzed Oligosaccharides in the Rhizome of Glycyrrhiza uralensis by Desorption Electrospray Ionization-Mass Spectrometry Imaging and High-Resolution Liquid Chromatography–Mass Spectrometry

Contact Info

Product

Resources

About