Spatial metabolomics identifies lipid profiles of human carotid atherosclerosis

Li, Wei; Luo, Jichang; Peng, Fangda; Liu, Ruiting; Bai, Xue‐Song; Wang, Tao; Zhang, Xiao; Zhu, Jingxian; Li, Xuying; Wang, Zhanjun; Liu, Wubin; Wang, Jiyue; Zhang, Liyong; Chen, Xianyang; Xue, Teng; Ding, Chunguang; Wang, Chaodong; Jiao, Liping

doi:10.1016/j.atherosclerosis.2022.11.019

Cited by 20 publications

(15 citation statements)

References 41 publications

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“…MYH11, a member of the myosin family, encoded the smooth muscle myosin heavy chain (SMMHC) and modulated the maturely differentiated SMCs in visceral tissues [36]. Overexpression of Protein inhibitor of activated STAT restrained proliferation and migration in vascular smooth muscle via promoting the expression of MYH11 [37]. Down-regulation of the long noncoding RNAMBNL1-AS1 increased the expression of KCNMA1, thereby regulating the proliferation and apoptosis in skeletal muscle cells via activation of the cGMP-PKG signaling pathway [38].…”

Section: Effects Of Dietary Protein Level On Gene Expression Of Rumen...mentioning

confidence: 99%

Effects of dietary crude protein on antioxidant activity, immunocompetence and the structural properties of the rumen in Tibetan sheep (Ovis aries), as determined by transcriptomic analysis

Wu,

Zhang,

et al. 2024

Preprint

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Background: The rumen is a balanced ecosystem harboring a variety of microorganisms and plays an important role in the digestion and absorption of nutrients for ruminant. However, there are few studies on the effects of dietary crude protein on development and function in rumen of Tibetan sheep. The objective of this study was to evaluate the effects of dietary crude protein (CP) on the antioxidant activity, immunocompetence and the structural properties in the rumen of Tibetan sheep. Sixty two-month-old rams with an average weight of 15.40±0.81 Kg were randomly assigned to low-protein diet (10.20% of dry matter, L group) and high-protein diet (11.58% of dry matter, H group). The experiment was conducted over 97 d, including 7 d of adaption to the diets. Results: Hematoxylin & eosin (H&E) results showed that high-protein diet increased papilla length, papilla width and muscular layer in rumen (P < 0.05). Compared with L group, supplementation with 11.58% crude protein increased the activities of T-AOC and SOD significantly (P < 0.05). A total of 612 significant differentially expressed genes (158 up-regulated and 456 down-regulated) were found in response to high-protein diet. Pathways and genes related to fatty acid biosynthesis, nutrition metabolism and muscle development were verified by real-time quantitative polymerase chain reaction. Conclusions: In conclusion, 11.58% crude protein diet had superior papillary development and antioxidant activity of Tibetan sheep, likely through modulating the expression of functional genes.

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Section: Effects Of Dietary Protein Level On Gene Expression Of Rumen...mentioning

confidence: 99%

Effects of dietary crude protein on antioxidant activity, immunocompetence and the structural properties of the rumen in Tibetan sheep (Ovis aries), as determined by transcriptomic analysis

Wu,

Zhang,

et al. 2024

Preprint

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“…7 Although considerable progress has been made over the past few years in studies related to the major components of carotid plaques and their pathomechanisms, our understanding of the metabolic processes within the metabolically disturbed plaque still lags behind. 3,8 Risk factors for carotid plaque formation and progression vary from country to country and region to region (e.g., ethnicity, genetic factors, and dietary habits), [9][10][11] and thus there may be marked heterogeneity in their plaque composition. To gain insight into the alteration of the plaque metabolic microenvironment in the pathogenesis and progression of the disease, as well as the risks associated with plaque instability, it is necessary to understand the biochemical mechanisms that lead to plaque accumulation and instability within a holistic framework.…”

Section: Introductionmentioning

confidence: 99%

“…It is well known that ischemic stroke is the leading cause of death and disability worldwide 1 . Currently, ischemic stroke derived from carotid atherosclerosis (CAS) and subsequent atherosclerotic plaque rupture is increasingly becoming a focus of scientific attention 2,3 . Most notably, three‐quarters of strokes caused by the carotid factor were previously without symptoms 4 .…”

Section: Introductionmentioning

confidence: 99%

Identification of metabolic components of carotid plaque in high‐risk patients utilizing liquid chromatography–tandem mass spectrometry

Ding,

Guan,

et al. 2024

Rapid Comm Mass Spectrometry

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ObjectiveCarotid atherosclerosis is a chronic progressive vascular disease that can be complicated by stroke in severe cases. Prompt diagnosis and treatment of high‐risk patients are quite difficult due to the lack of reliable clinical biomarkers. This study aimed to explore potential plaque metabolic markers of stroke‐prone risk and relevant targets for pharmacological intervention.MethodCarotid intima and plaque sample tissues were obtained from 20 patients with cerebrovascular symptoms of carotid origin. An untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry was utilized to characterize the metabolic profiles of the tissues. Multivariate and univariate analysis tools were used.ResultsA total of 154 metabolites were significantly altered in carotid plaque when compared with thickened intima. Of these, 62 metabolites were upregulated, whereas 92 metabolites were downregulated. Support vector machines identified the 15 most important metabolites, such as N‐(cyclopropylmethyl)‐N′‐phenylurea, 9(S)‐HOTrE, ACar 12:2, quinoxaline‐2,3‐dithiol, and l‐thyroxine, as biomarkers for high‐risk plaques. Metabolic pathway analysis showed that abnormal purine and nucleotide metabolism, amino acid metabolism, glutathione metabolism, and vitamin metabolism may contribute to the occurrence and progression of carotid atherosclerotic plaque.ConclusionsOur study identifies the biomarkers and related metabolic mechanisms of carotid plaque, which is stroke‐prone, and provides insights and ideas for the precise prevention and targeted intervention of the disease.

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“…In the past few years, the urgent demands of single-cell , and spatial metabolomics studies − have called for novel analytical tools for sensitive mass spectrometric (MS) analysis of ultra-small-volume samples. − For instance, spatial metabolomics studies usually involve analysis of metabolites, drugs, and lipids , in an individual cell and its microenvironment (micro-tissue section) with typical sample amount of nano- to picoliter levels. Micro-biopsy had been proved to be an effective method to acquire such amount of samples from tumor tissues and cells, nerve cells, microsection of plants, etc.…”

Section: Introductionmentioning

confidence: 99%

Direct Analysis of Micro-biopsy Samples by Polarity Gradient Focusing Dip-and-Go Mass Spectrometry

Han,

Wang,

Wang

et al. 2023

Anal. Chem.

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Direct analysis of micro-biopsy samples by mass spectrometry at single-cell level still faces major challenges. In this work, we developed a polarity gradient focusing dip-and-go strategy (PGF-Dip&Go) during induced electrospray ionization mass spectrometry (iESI-MS) analysis for real-time enrichment and spatial separation of compounds such as lipids, alkaloids, fatty amines, and drugs. Compared with direct iESI-MS analysis, enrichment of analytes (enrichment factor of 5.0−100.0) and spatial separation between different analytes were achieved. Owing to the enrichment effect and salt cleanup effect, the sensitivity of PGF-Dip&Go has been improved by 25−10,000 times compared with direct iESI-MS. PGF-Dip&Go has been successfully applied for the analysis of lipids in a 200 pL micro-biopsy section from an individual fish egg. Lysophosphatidylcholine (LPC), phosphatidylcholine (PC), and triglyceride (TG) were significantly enriched and separated according to their polarity differences, proving the potential of PGF-Dip&Go to be a noninvasive and powerful analytical tool for in situ analysis of complex small volumes in the future.

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Spatial metabolomics identifies lipid profiles of human carotid atherosclerosis

Cited by 20 publications

References 41 publications

Effects of dietary crude protein on antioxidant activity, immunocompetence and the structural properties of the rumen in Tibetan sheep (Ovis aries), as determined by transcriptomic analysis

Effects of dietary crude protein on antioxidant activity, immunocompetence and the structural properties of the rumen in Tibetan sheep (Ovis aries), as determined by transcriptomic analysis

Identification of metabolic components of carotid plaque in high‐risk patients utilizing liquid chromatography–tandem mass spectrometry

Direct Analysis of Micro-biopsy Samples by Polarity Gradient Focusing Dip-and-Go Mass Spectrometry

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