Spatial Organization and Coordination of the Plant Circadian System

Nohales, María A.

doi:10.3390/genes12030442

Cited by 15 publications

(23 citation statements)

References 81 publications

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“…In mammals, such multiple tissue‐specific oscillators are organized hierarchically, where a central clock located in the suprachiasmatic nucleus (SCN) of the brain provides temporal information that synchronizes peripheral oscillators (Mohawk et al., 2012; Tomioka and Matsumoto, 2010). Considerable evidence supports the model that plants also have multiple oscillators regulated tissue‐specifically (Endo, 2016; Nohales, 2021; Sorkin and Nusinow, 2021). One of the first examples was shown in tobacco, where rhythms in cytosolic free calcium and LHCB ( CAB ) gene expression were shown to exhibit different free‐running periods (Sai and Johnson, 1999; Figure 2b).…”

Section: Circadian Time: Are There Local Time Zones Within Plant Tiss...mentioning

confidence: 88%

“…oscillators regulated tissue-specifically (Endo, 2016;Nohales, 2021;Sorkin and Nusinow, 2021). One of the first examples was shown in tobacco, where rhythms in cytosolic free calcium and LHCB (CAB) gene expression were shown to exhibit different free-running periods (Sai and Johnson, 1999; Figure 2b).…”

Section: Tissue-specific Timekeepingmentioning

confidence: 99%

“…Similarly, rhythmic expression of the clock component CCA1 exhibited delayed phasing and longer periods in guard cells compared with adjacent mesophyll and epidermal cells, again supporting tissue specificity in oscillator function (Yakir et al., 2011). Crucially, the discovery of rhythms with distinct periods demonstrates the presence of multiple oscillators (Nohales, 2021). This is because while a single clock can drive rhythms with multiple phases, it cannot generate multiple periods, as the period is an intrinsic property of the oscillator.…”

Section: Circadian Time: Are There Local Time Zones Within Plant Tiss...mentioning

confidence: 99%

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The biology of time: dynamic responses of cell types to developmental, circadian and environmental cues

et al. 2021

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As sessile organisms, plants are finely tuned to respond dynamically to developmental, circadian and environmental cues. Genome-wide studies investigating these types of cues have uncovered the intrinsically different ways they can impact gene expression over time. Recent advances in single-cell sequencing and time-based bioinformatic algorithms are now beginning to reveal the dynamics of these time-based responses within individual cells and plant tissues. Here, we review what these techniques have revealed about the spatiotemporal nature of gene regulation, paying particular attention to the three distinct ways in which plant tissues are time sensitive. (i) First, we discuss how studying plant cell identity can reveal developmental trajectories hidden in pseudotime. (ii) Next, we present evidence that indicates that plant cell types keep their own local time through tissue-specific regulation of the circadian clock. (iii) Finally, we review what determines the speed of environmental signaling responses, and how they can be contingent on developmental and circadian time. By these means, this review sheds light on how these different scales of timebased responses can act with tissue and cell-type specificity to elicit changes in whole plant systems.

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Section: Circadian Time: Are There Local Time Zones Within Plant Tiss...mentioning

confidence: 88%

Section: Tissue-specific Timekeepingmentioning

confidence: 99%

Section: Circadian Time: Are There Local Time Zones Within Plant Tiss...mentioning

confidence: 99%

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The biology of time: dynamic responses of cell types to developmental, circadian and environmental cues

et al. 2021

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“…4,5 Physiological rhythms with different free-running periods (FRPs) have been observed in plants. 6 For example, rhythms in cytosolic free calcium (Ca 2+ ) levels have a different FRP from that of Lhcb gene expression in tobacco. 7 Differences in the FRPs of bioluminescence rhythms were observed in Arabidopsis seedlings expressing a firefly luciferase under circadian promoters CHALCONE SYNTHASE (CHS), CHLOROPHYLL A/B BINDING PROTEIN (CAB), PHYTOCHROME B (PHYB) and CATALASE 3 (CAT3).…”

Section: Introductionmentioning

confidence: 99%

No circadian clock-gene circuit in the generation of cellular bioluminescence rhythm of CaMV35S::PtRLUC in duckweed

Watanabe

Muranaka

Nakamura

et al. 2022

Preprint

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SummaryThe circadian clock is responsible for the temporal regulation of various physiological phenomena in plants. Individual cells contain the cellular circadian clock consisting of the clock gene circuit. Physiological rhythms are coordinated in the plant body in an orderly manner. Multiple circadian rhythms with different free running periods (FRPs) can coexist in the same plant. In addition to different circadian properties between tissues/organs, uncoupled circadian rhythms in the same cell have been reported. The relationship between the cellular circadian clock and the various circadian outputs with different FRPs is currently unknown. Using a dual-color bioluminescence monitoring system in Lemna minor transfected with the AtCCA1::LUC+ and CaMV35S::PtRLUC reporters, cellular bioluminescence rhythms with different FRPs were detected in the same cells. Co-transfection experiments with the two reporters and a clock gene overexpressing effector revealed that the circadian properties of the AtCCA1::LUC+ rhythm, but not those of the CaMV35S::PtRLUC rhythm, were altered in the cells with a dysfunctional clock gene circuit. This indicates that the AtCCA1::LUC+ rhythm is a direct output of the cellular circadian clock while the CaMV35S::PtRLUC rhythm is not. After plasmolysis, the CaMV35S::PtRLUC rhythm disappeared while the AtCCA1::LUC+ rhythm persisted. This suggested that CaMV35S::PtRLUC is a symplast/apoplast mediated circadian rhythm generated at an organismal level. This idea is consistent with the observation that the phases of CaMV35S::PtRLUC cellular rhythms remain highly synchronized while those of AtCCA1::LUC+ rhythms gradually get desynchronized. The plant circadian system consists of both cell-autonomous rhythms and non-cell-autonomous rhythms that are unaffected by the cellular clock.

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“…Basically, the morning expressed CCA1 -like MYB s, CCA1 and its homolog LATE ELONGATED HYPOCOTYL 1 ( LHY1 ), serve as central oscillators to control circadian rhythms directly through repressing the afternoon expressed PSEUDO-RESPONSE REGULATOR ( PRR ) genes. Sequentially, the midday-expressed CCA1 -like MYB s, REVEILLE ( RVE ) genes such as RVE4 , RVE6 , and RVE8 , can activate the expressions of the oscillator genes including PRR s and the evening-expressed genes ( Nohales, 2021 ). Recently, it has been proposed that the balance between the activating and repressing MYB-like factors is more important in the regulation of the circadian clock than the presence or absence of any specific factor ( Carre and Kim, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis

et al. 2021

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REVEILLE (RVE) genes generally act as core circadian oscillators to regulate multiple developmental events and stress responses in plants. It is of importance to document their roles in crops for utilizing them to improve agronomic traits. Soybean is one of the most important crops worldwide. However, the knowledge regarding the functional roles of RVEs is extremely limited in soybean. In this study, the soybean gene GmMYB133 was shown to be homologous to the RVE8 clade genes of Arabidopsis. GmMYB133 displayed a non-rhythmical but salt-inducible expression pattern. Like AtRVE8, overexpression of GmMYB133 in Arabidopsis led to developmental defects such as short hypocotyl and late flowering. Seven light-responsive or auxin-associated genes including AtPIF4 were transcriptionally depressed by GmMYB133, suggesting that GmMYB133 might negatively regulate plant growth. Noticeably, the overexpression of GmMYB133 in Arabidopsis promoted seed germination and plant growth under salt stress, and the contents of chlorophylls and malondialdehyde (MDA) were also enhanced and decreased, respectively. Consistently, the expressions of four positive regulators responsive to salt tolerance were remarkably elevated by GmMYB133 overexpression, indicating that GmMYB133 might confer salt stress tolerance. Further observation showed that GmMYB133 overexpression perturbed the clock rhythm of AtPRR5, and yeast one-hybrid assay indicated that GmMYB133 could bind to the AtPRR5 promoter. Moreover, the retrieved ChIP-Seq data showed that AtPRR5 could directly target five clients including AtPIF4. Thus, a regulatory module GmMYB133-PRR5-PIF4 was proposed to regulate plant growth and salt stress tolerance. These findings laid a foundation to further address the functional roles of GmMYB133 and its regulatory mechanisms in soybean.

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Spatial Organization and Coordination of the Plant Circadian System

Cited by 15 publications

References 81 publications

The biology of time: dynamic responses of cell types to developmental, circadian and environmental cues

The biology of time: dynamic responses of cell types to developmental, circadian and environmental cues

No circadian clock-gene circuit in the generation of cellular bioluminescence rhythm of CaMV35S::PtRLUC in duckweed

Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis

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