Human-wildlife interactions are present wherever both humans and wildlife are found. Interactions can be positive or negative for humans and can include impacts that range from damage to property, agriculture, health and human safety, to emotional effects. Livestock-wildlife interactions form a major component of human-wildlife conflicts with foci often centered on the implications of livestock predation by wildlife.While most vulture species are obligate scavengers, several species, including the American black vulture (Coragyps atratus), may attack weak or newborn livestock. Black vulture populations and distributions are expanding in the Midwestern U.S., and attacks create a novel problem for livestock producers. We used online and printed surveys to determine the experiences and perceived losses of producers in Indiana and Kentucky. Surveys were distributed March-July 2021.Losses to black vultures were reported by 22% of goat producers, 24% of sheep producers, 38% of cattle producers, and 44% of mixed-livestock producers. The criteria used to determine a perceived predation event were presence of vultures on the carcass, followed by missing eyes or tongue, damage to the perineal area, reported visual observation of the kill, or presence of feathers around the carcass. Sixty-two