Investigating regional connections and their influencing factors from the perspective of "flow" space is one of the foundations of promoting regional development. In this article, the data we used includes actual highway passenger flow data, National Polar-orbiting Partnership/Visible Infrared Imaging Radiometer Suite (NPP/VIIRS) nighttime light remote sensing data, and socioeconomic data. We analyzed the spatial distribution pattern, connection intensity and spatial autocorrelation of highway passenger flow in Chongqing during the working day, weekend and May Day and revealed the influencing factors by means of a geographic detector. Three key conclusions resulted from this research. First, highway passenger flow in Chongqing districts exhibits spatial agglomeration that is clearly higher in western Chongqing than in eastern Chongqing and forms an obviously dual-core "star" structure, with the main urban area and Wanzhou serving as the core. Second, a factor detector notes that the nighttime light area index (0.9251, 0.9512, 0.9541) has the strongest explanatory power for the spatial differentiation of passenger traffic in Chongqing districts, which is the key factor. Third, interaction detection shows that the interaction between the two factors displays an enhancement effect at different times. The nighttime light area index shows the strongest explanatory power under the synergy of tourist attractions, which are 0.9850, 0.9903 and 0.9908. But the per capita GDP and highway mileage have the most obvious enhancement effect after interaction (0.9544, 0.9661, 0.9652). Therefore, in future planning and development, we should pay attention to cooperation and exchanges between districts and use the nighttime light area index as an important reference factor to provide a scientific basis for the construction of public transport and economic construction in Chongqing.