The campus, as an educational area, has a variety of land cover with varying surface temperatures. Knowledge of land use in a localized urban environment and its effect on the Urban Heat Signature (UHS) of a university campus is insufficient, so it is essential to assess UHS-related localized urban environments. The objective of this study is to assess land cover and its effect on the UHS of two university campuses. The research used spatial-temporal analysis employing satellite images during the period 2013-2014. The areas studied were the University Malaya (UM) and University Indonesia (UI) campuses. The results show that the land cover of both university campuses has the same localized urban environment pattern. The study concludes that the UHS behavior is an effect of its temporal relation with land cover, which is new knowledge on university campuses about localized urban environments.