Spatial transcriptomic interrogation of the tumour-stroma boundary in a 3D engineered model of ameloblastoma

Bakkalci, Deniz; Al-Badri, Georgina; Yang, Wei; Nam, Andy; Liang, Yan; Khurram, Syed Ali; Heavey, Susan; Fedele, Stefano; Cheema, Umber

doi:10.1016/j.mtbio.2023.100923

Materials Today Bio

2024

DOI: 10.1016/j.mtbio.2023.100923

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Spatial transcriptomic interrogation of the tumour-stroma boundary in a 3D engineered model of ameloblastoma

Deniz Bakkalci,

Georgina Al-Badri,

Wei Yang

et al.

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2024

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Cited by 2 publications

References 42 publications

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Exploiting BRAF mutations in the therapeutic approach towards oral and maxillofacial tumors

Yi,

Zhong,

Lin

et al. 2024

Journal of Stomatology, Oral and Maxillofacial Surgery

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Exploiting BRAF mutations in the therapeutic approach towards oral and maxillofacial tumors

Yi,

Zhong,

Lin

et al. 2024

Journal of Stomatology, Oral and Maxillofacial Surgery

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Dense Collagen I as a Biomimetic Material to Track Matrix Remodelling in Renal Carcinomas

Upadhyay,

Bakkalci,

Micalet

et al. 2024

ACS Omega

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Renal tissue is a dynamic biophysical microenvironment, regulating healthy function and influencing tumor development. Matrix remodelling is an iterative process and aberrant tissue repair is prominent in kidney fibrosis and cancer. Biomimetic 3D models recapitulating the collagen composition and mechanical fidelity of native renal tissue were developed to investigate cell−matrix interactions in renal carcinomas. Methods: Collagen I and laminin hydrogels were engineered with renal cancer cells (ACHN and 786-O), which underwent plastic compression to generate dense matrices. Mechanical properties were determined using shear rheology and qPCR determined the gene expression of matrix markers. Results: The shear modulus and phase angle of acellular dense collagen I gels (474 Pa and 10.7) are similar to human kidney samples (1410 Pa and 10.5). After 21 days, 786-O cells softened the dense matrix (∼155 Pa), with collagen IV downregulation and upregulation of matrix metalloproteinases (MMP7 and MMP8). ACHN cells were found to be less invasive and stiffened the matrix to ∼1.25 kPa, with gene upregulation of collagen IV and the cross-linking enzyme LOX. Conclusions: Renal cancer cells remodel their biophysical environment, altering the material properties of tissue stroma in 3D models. These models can generate physiologically relevant stiffness to investigate the different matrix remodelling mechanisms utilized by cancer cells.

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Spatial transcriptomic interrogation of the tumour-stroma boundary in a 3D engineered model of ameloblastoma

Cited by 2 publications

References 42 publications

Exploiting BRAF mutations in the therapeutic approach towards oral and maxillofacial tumors

Exploiting BRAF mutations in the therapeutic approach towards oral and maxillofacial tumors

Dense Collagen I as a Biomimetic Material to Track Matrix Remodelling in Renal Carcinomas

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