Spatial transcriptomics of a giant pilomatricoma

Abstract

Pilomatricomas (PMs) are common benign adnexal tumors that show a predilection for the head and neck region and are characterized at the molecular level by activating mutations in the beta‐catenin (CTNNB1) gene. Giant PMs are a rare histopathological variant, according to the World Health Organization, which are defined by a size greater than 4 cm and are reported to show upregulation of yes‐associated protein compared to PMs of typical 1–3 cm size. We describe the case of a 67‐year‐old man with an 8 cm giant PM involving his temporal scalp, whose PM we characterized by 10X spatial gene expression analysis. This revealed five total transcriptomic clusters, including four distinct clusters within the giant PM, each with a unique transcriptional pattern of hair follicle‐related factors, keratin gene expression, and beta‐catenin pathway activity.

Unique Spatial Transcriptomic Profiling of the Murine Femoral Fracture Callus: A Preliminary Report

“…The DV200 index assesses a percentage of RNA fragments greater than 200 nucleotides, which has shown greater correlation with RNA quality than the traditional RNA integrity number [ 50 ]. Previous literature studies involving this platform have ranged from one to four replicates per group [ 51 , 52 , 53 , 54 , 55 ]. However, power analysis is challenging given that spatial features are unknown as well as the high complexity of spatial data [ 56 ].…”