Spatially resolved metabolomics combined with bioactivity analyses to evaluate the pharmacological properties of two Radix Puerariae species

Guo, Na; Fang, Zhengyu; Zang, Qingce; Yang, Yi‐Qing; Nan, Tie-Gui; Zhao, Yuping; Huang, Luqi

doi:10.1016/j.jep.2023.116546

Cited by 11 publications

(4 citation statements)

References 36 publications

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“…As a standardized Chinese Materia Medica product approved by the China National Medical Products Administration, the quality control of EEPL complied with Chinese Pharmacopoeia standards. To qualitatively reveal the material basis of EEPL, metabolomic profile analysis was performed using a Waters Xevo G2XS Q‐TOF system (Waters Corp., Milford, MA) equipped with an electrospray ionization (ESI) source, described in our previous study (Guo et al, 2023). Acetonitrile and formic acid (MS grade) were purchased from Fisher Scientific.…”

Section: Methodsmentioning

confidence: 99%

Ethanol extract of Pueraria lobata improve acute myocardial infarction in rats via regulating gut microbiota and bile acid metabolism

Zhang,

Fang,

Zhao

et al. 2023

Phytotherapy Research

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Background and AimAcute myocardial infarction (AMI) is a multifactorial disease with high mortality rate worldwide. Ethanol extract of Pueraria lobata (EEPL) has been widely used in treating cardiovascular diseases in China. This study aimed to explore the underlying therapeutic mechanism of EEPL in AMI rats.Experimental ProcedureWe first evaluated the anti‐AMI efficacy of EEPL through immunohistochemistry staining and biochemical indexes. Then, UPLC‐MS/MS, 16S rDNA, and shotgun metagenomic sequencing were used to analyze the alterations in bile acid metabolism and intestinal flora. Finally, the influence of EEPL on ilem bile acid metabolism, related enzymes expression, and transporter proteins expression in rats were verified by mass spectrometry image and ELISA.Key ResultsThe results showed that EEPL can reduce cardiac impairment in AMI rats. Besides, EEPL effectively increased bile acid levels and regulated gut microbiota disturbance in AMI rats via increasing CYP7A1 expression and restoring intestinal microbiota diversity, separately. Moreover, it can increase bile acids reabsorption and fecal excretion through inhibiting FXR‐FGF15 signaling pathway and increasing OST‐α expression, which associated with Lachnoclostridium.Conclusions and ImplicationsOur findings demonstrated that EEPL alleviated AMI partially by remediating intestinal dysbiosis and promoting bile acid biosynthesis, which provided new targets for AMI treatment.

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Section: Methodsmentioning

confidence: 99%

Ethanol extract of Pueraria lobata improve acute myocardial infarction in rats via regulating gut microbiota and bile acid metabolism

Zhang,

Fang,

Zhao

et al. 2023

Phytotherapy Research

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“…This is an effective method for in-depth analysis and understanding of TCM composition and metabolite biosynthesis ( Huang et al, 2022 ). Guo et al, 2023 utilized AFADESI-MSI to detect the metabolites in Pueraria montana (Lour.) Merr.…”

Section: Approaches For Establishing Amctmentioning

confidence: 99%

“…This is an effective method for in-depth analysis and understanding of TCM composition and metabolite biosynthesis (Huang et al, 2022). Guo et al, 2023…”

Section: Identifying Formulation Metabolitesmentioning

confidence: 99%

Active metabolites combination therapies: towards the next paradigm for more efficient and more scientific Chinese medicine

Gao,

Wu,

Chen

et al. 2024

Front. Pharmacol.

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Traditional Chinese medicine (TCM) formulae have been studied extensively in various human diseases and have proven to be effective due to their multi-component, multi-target advantage. However, its active metabolites are not clear and the specific mechanisms are not well established, which limits its scientific application. Recently, combination therapies are attracting increasing attention from the scientific community in the past few years and are considered as the next paradigm in drug discovery. Here, we tried to define a new concept of “active metabolites combination therapies (AMCT)” rules to elucidate how the bioactive metabolites from TCMs to produce their synergistic effects in this review. The AMCT rules integrate multidisciplinary technologies like molecular biology, biochemistry, pharmacology, analytical chemistry and pharmacodynamics, etc. Meanwhile, emerging technologies such as multi-omics combined analysis, network analysis, artificial intelligence conduce to better elucidate the mechanisms of these combination therapies in disease treatment, which provides new insights for the development of novel active metabolites combination drugs. AMCT rules will hopefully further guide the development of novel combination drugs that will promote the modernization and international needs of TCM.

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“…Other recent MSI studies on medicinal plants have spatially mapped diverse phenolics in Cannabis sativa leaves (discussed below with ‘Other metabolites’) ( Lorensen et al , 2023b ), Ginkgo biloba leaves ( Li et al , 2018 ), dried fruits of Forsythia suspensa ( Jing et al , 2022 ), dried roots of two Radix puerariae species ( Guo et al , 2023 ), and Paeonia sp. roots ( Li et al , 2021 ).…”

Section: Expanding the Spatial Metabolome Coveragementioning

confidence: 99%

Imaging plant metabolism in situ

Horn,

Chapman

2023

Journal of Experimental Botany

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Mass spectrometry imaging (MSI) has emerged as an invaluable analytical technique for investigating the spatial distribution of molecules within biological systems. In the realm of plant science, MSI is increasingly employed to explore metabolic processes across a wide array of plant tissues, including those in leaves, fruits, stems, roots, and seeds, spanning various plant systems such as model species, staple and energy crops, and medicinal plants. By generating spatial maps of metabolites, MSI has elucidated the distribution patterns of diverse metabolites and phytochemicals, encompassing lipids, carbohydrates, amino acids, organic acids, phenolics, terpenes, alkaloids, vitamins, pigments, and others, thereby providing insights into their metabolic pathways and functional roles. In this review, we present recent MSI studies that demonstrate the advances made in visualizing the plant spatial metabolome. Moreover, we emphasize the technical progresses that enhance the identification and interpretation of spatial metabolite maps. Within a mere decade since the inception of plant MSI studies, this robust technology is poised to continue as a vital tool for tackling complex challenges in plant metabolism.

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Spatially resolved metabolomics combined with bioactivity analyses to evaluate the pharmacological properties of two Radix Puerariae species

Cited by 11 publications

References 36 publications

Ethanol extract of Pueraria lobata improve acute myocardial infarction in rats via regulating gut microbiota and bile acid metabolism

Ethanol extract of Pueraria lobata improve acute myocardial infarction in rats via regulating gut microbiota and bile acid metabolism

Active metabolites combination therapies: towards the next paradigm for more efficient and more scientific Chinese medicine

Imaging plant metabolism in situ

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