Spatially resolved small-angle X-ray scattering for characterizing mechanoresponsive liposomes using microfluidics

Buscema, Marzia; Deyhle, Hans; Pfohl, Thomas; Müller, Bert

doi:10.1016/j.mtbio.2019.100003

Cited by 11 publications

(19 citation statements)

References 44 publications

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“…The anisotropy in the scattering pattern makes it possible to quantify degree and angle of orientation as observed before in other systems. [ 28,29 ]…”

Section: Introductionmentioning

confidence: 99%

In Situ Visualization of the Structural Evolution and Alignment of Lyotropic Liquid Crystals in Confined Flow

Rodríguez-Palomo

Lutz‐Bueno

Cao

et al. 2021

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Self‐assembled materials such as lyotropic liquid crystals offer a wide variety of structures and applications by tuning the composition. Understanding materials behavior under flow and the induced alignment is wanted in order to tailor structure related properties. A method to visualize the structure and anisotropy of ordered systems in situ under dynamic conditions is presented where flow‐induced nanostructural alignment in microfluidic channels is observed by scanning small angle X‐ray scattering in hexagonal and lamellar self‐assembled phases. In the hexagonal phase, the material in regions with high extensional flow exhibits orientation perpendicular to the flow and is oriented in the flow direction only in regions with a high enough shear rate. For the lamellar phase, a flow‐induced morphological transition occurs from aligned lamellae toward multilamellar vesicles. However, the vesicles do not withstand the mechanical forces and break in extended lamellae in regions with high shear rates. This evolution of nanostructure with different shear rates can be correlated with a shear thinning viscosity curve with different slopes. The results demonstrate new fundamental knowledge about the structuring of liquid crystals under flow. The methodology widens the quantitative investigation of complex structures and identifies important mechanisms of reorientation and structural changes.

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“…The anisotropy in the scattering pattern makes it possible to quantify degree and angle of orientation as observed before in other systems. [ 28,29 ]…”

Section: Introductionmentioning

confidence: 99%

In Situ Visualization of the Structural Evolution and Alignment of Lyotropic Liquid Crystals in Confined Flow

Rodríguez-Palomo

Lutz‐Bueno

Cao

et al. 2021

Small

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“…Thea mide moieties allow additional tight hydrogen-bonding interactions between the phospholipids.F urthermore,t he increased spacing of nonnatural 1,3-arrangement between the lipid tails allows for an interdigitation of the two bilayer leaflets. [17] This leads to very stiff bilayers in the gel phase and therefore significant changes in the vesicle morphology that is now heavily faceted (Supporting Information, Figure S1). We then coated Rad-PC-Rad nanovesicles with gold (Au-m-nV) to obtain nearinfrared plasmon resonances from 700 to 900 nm (Figure 2b) and observed hydrodynamic diameter increase due to gold-coating by dynamic light scattering (Supporting Information, Figure S1).…”

Section: Resultsmentioning

confidence: 99%

“…Less than 13 %release was measured at 37 8 8Cfor Au-m-nV.T he high stability of Au-m-nV is attributed to the high main phase transition temperature of Rad-PC-Rad at 44.7 8 8Ca nd the tight interdigitation of lipid chains. [17] The nanovesicles were then vortexed using al aboratory vortex mixer (Figure 2e). Both the m-nV and Au-m-nV released the encapsulated dye molecules,w hile no release was observed for DPPC vesicles in the presence or absence of gold-coating (Au-nV and nV).…”

Section: Resultsmentioning

confidence: 99%

“…The amide moieties allow additional tight hydrogen‐bonding interactions between the phospholipids. Furthermore, the increased spacing of non‐natural 1,3‐arrangement between the lipid tails allows for an interdigitation of the two bilayer leaflets . This leads to very stiff bilayers in the gel phase and therefore significant changes in the vesicle morphology that is now heavily faceted (Supporting Information, Figure S1).…”

Section: Resultsmentioning

confidence: 99%

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Near‐Infrared Light Triggered‐Release in Deep Brain Regions Using Ultra‐photosensitive Nanovesicles

Xiong

Kang

et al. 2020

Angewandte Chemie

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Remote and minimally‐invasive modulation of biological systems with light has transformed modern biology and neuroscience. However, light absorption and scattering significantly prevents penetration to deep brain regions. Herein, we describe the use of gold‐coated mechanoresponsive nanovesicles, which consist of liposomes made from the artificial phospholipid Rad‐PC‐Rad as a tool for the delivery of bioactive molecules into brain tissue. Near‐infrared picosecond laser pulses activated the gold‐coating on the surface of nanovesicles, creating nanomechanical stress and leading to near‐complete vesicle cargo release in sub‐seconds. Compared to natural phospholipid liposomes, the photo‐release was possible at 40 times lower laser energy. This high photosensitivity enables photorelease of molecules down to a depth of 4 mm in mouse brain. This promising tool provides a versatile platform to optically release functional molecules to modulate brain circuits.

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“…recently characterized the structural changes of faceted Pad‐PC‐Pad liposomes under shear through spatially resolved small‐angle X‐ray scattering in a microfluidic device mimicking a stenotic blood vessel. [ 50 ] Their results indicated that the gradient pressure force upstream and downstream of the stenotic sites, rather than the wall shear stress in the blood vessel, provoked the structural changes of the liposomes and subsequent drug release. In an effort to improve their stability in the human body, Pad‐PC‐Pad liposomes have been further modified.…”

Section: Shear‐deformable Npsmentioning

confidence: 99%

Recent Developments in Nanomaterial‐Based Shear‐Sensitive Drug Delivery Systems

Wang

Pisapati

Zhang

et al. 2021

Adv Healthcare Materials

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Nanomaterial‐based drug delivery systems (DDSs) increase the efficacy of various therapeutics, and shear stress has been shown to be a robust modulator of payload release. In the past few decades, a deeper understanding has been gained of the effects of flow in the body and its alteration in pathological microenvironments. More recently, shear‐responsive nanomaterial DDSs have been developed. Studies on this subject mainly from the last decade are reviewed here, focusing on innovations of the material design and mechanisms of the shear response. The two most popular shear‐controlled drug carriers distinguished by different release mechanisms, that is, shear‐deformable nanoparticles (NPs) and shear‐dissociated NP aggregates (NPAs), are surveyed. The influence of material structures on their properties such as drug loading, circulation time, and shear sensitivity are discussed. The drug development stages, therapeutic effects, limitations, and potential of these DDSs are further inspected. The reviewed research emphasizes the advantages and significance of nanomaterial‐based shear‐sensitive DDSs in the field of targeted drug delivery. It is also believed that efforts to rationally design nanomaterial DDSs responsive to shear may prompt a new class of diagnostics and therapeutics for signaling and rectifying pathological flows in the body.

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Spatially resolved small-angle X-ray scattering for characterizing mechanoresponsive liposomes using microfluidics

Cited by 11 publications

References 44 publications

In Situ Visualization of the Structural Evolution and Alignment of Lyotropic Liquid Crystals in Confined Flow

In Situ Visualization of the Structural Evolution and Alignment of Lyotropic Liquid Crystals in Confined Flow

Near‐Infrared Light Triggered‐Release in Deep Brain Regions Using Ultra‐photosensitive Nanovesicles

Recent Developments in Nanomaterial‐Based Shear‐Sensitive Drug Delivery Systems

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