2010
DOI: 10.1117/12.838287
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Spatio-temporal control in multiphoton fluorescence laser-scanning microscopy

Abstract: We present a clever design concept of using femtosecond laser pulses in microscopy by selective excitation or de-excitation of one fluorophore over the other overlapping one. Using either a simple pair of femtosecond pulses with variable delay or using a train of laser pulses at 20-50 Giga-Hertz excitation, we show controlled fluorescence excitation or suppression of one of the fluorophores with respect to the other through wave-packet interference, an effect that prevails even after the fluorophore coherence … Show more

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Cited by 5 publications
(3 citation statements)
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“…However, control can also be achieved beyond coherence time scales using stimulated emission, as recently shown by our group. 11,12 Extending the previous work using pulse-pair excitation, here we demonstrate how 20-to 50-GHz pulse-train excitation can selectively suppress fluorescence from one particular fluorophore by stimulated emission, which is otherwise not possible using a pulse-pair scheme.…”
Section: Introductionmentioning
confidence: 99%
“…However, control can also be achieved beyond coherence time scales using stimulated emission, as recently shown by our group. 11,12 Extending the previous work using pulse-pair excitation, here we demonstrate how 20-to 50-GHz pulse-train excitation can selectively suppress fluorescence from one particular fluorophore by stimulated emission, which is otherwise not possible using a pulse-pair scheme.…”
Section: Introductionmentioning
confidence: 99%
“…14 Control is achieved by simultaneous two-photon absorption (TPA) by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. 15 Recently we have also demonstrated selective fluorescence suppression by pulse-train excitation where, instead of two time-delayed identical pulses, many pulses with gradually decreasing pulse intensities (in geometric progression) having a controllable delay between successive pulses, were employed. 16 Here the first pulse leads to TPA while one-photon stimulated emission takes over TPA for successive pulses.…”
Section: Introductionmentioning
confidence: 99%
“…(incoherent) population dynamics, where the only “control knob” is the time delay between the pairs. Earlier our group reported selective TPF suppression using a pulse-pair excitation scheme 13 which was explained based on selective stimulated emission by a time-delayed second pulse following the excitation pulse; 14 the control is achieved by simultaneous two-photon absorption (TPA) by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. Here we further explore the mechanistic detail of such one-color control scheme in depth.…”
Section: Introductionmentioning
confidence: 99%