Spatio-temporal distribution of naturally occurringBacillus spp. and other bacteria on the phylloplane of soybean under field conditions

Arias, Renée S.; Sagardoy, Marcelo Antonio; Vuurde, J.W.L. van

doi:10.1002/(sici)1521-4028(199912)39:5/6<283::aid-jobm283>3.0.co;2-g

Cited by 38 publications

(14 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Argentina, a reduction in the diversity of Bacillus spp. from the soybean phyllosphere occurred throughout the season, with only one species (B. pumilus) being recovered late in the season (2). The role of heat and dessiccation stresses in the seasonal succession observed in phyllosphere bacterial communities remains an open question.…”

Section: Discussioncontrasting

confidence: 60%

Effect of Solar UV-B Radiation on a Phyllosphere Bacterial Community

Jacobs

Sundin

2001

Appl Environ Microbiol

143

View full text Add to dashboard Cite

The effect of solar UV-B radiation on the population dynamics and composition of the culturable bacterial community from peanut (Arachis hypogeae L.) was examined in field studies using plants grown under UV-B؊transmitting (UV-B؉) or UV-B؊excluding (UV-B؊) plastic filters. Our data demonstrate that solar UV-B selection alters phyllosphere bacterial community composition and that UV tolerance is a prevalent phenotype late in the season. The total bacterial population size was not affected by either UV-B treatment. However, isolates from the UV-B؉ plots (n ‫؍‬ 368) were significantly more UV tolerant than those from the UV-B؊ (n ‫؍‬ 363) plots. UV sensitivity was determined as the minimal inhibitory dose of UV that resulted in an inhibition of growth compared to the growth of a nonirradiated control. The difference in minimal inhibitory doses among bacterial isolates from UV-B؉ and UV-B؊ treatments was mainly partitioned among nonpigmented isolates, with pigmented isolates as a group being characterized as UV tolerant. A large increase in UV tolerance was observed within isolate groups collected late (89 and 96 days after planting) in the season. Identification of 200 late-season isolates indicated that the predominant UV-tolerant members of this group were Bacillus coagulans, Clavibacter michiganensis, and Curtobacterium flaccumfaciens. We selected C. michiganensis as a model UV-tolerant epiphyte to study if cell survival on UV-irradiated peanut leaves was increased relative to UV survival in vitro. The results showed an enhancement in the survival of C. michiganensis G7.1, especially following high UV-C doses (300 and 375 J m ؊2 ), that was evident between 24 and 96 h after inoculation. A dramatic increase in the in planta/in vitro survival ratio was observed over the entire 96-h experiment period for C. michiganensis T5

show abstract

Section: Discussioncontrasting

confidence: 60%

Effect of Solar UV-B Radiation on a Phyllosphere Bacterial Community

Jacobs

Sundin

2001

Appl Environ Microbiol

143

View full text Add to dashboard Cite

show abstract

“…Bacillus species are typical inhabitants of the rhizosphere, but they are also commonly present on the surface of plants. B. subtilis and B. megaterium had been detected in the olive phylloplane at all times during the year[20], and B. pumilis was found to be an efficient colonizer of the soybean phylloplane[21]. B. pumilis was isolated from the phyllosphere of an apple orchard in Northern Germany and was an efficient antagonist to Venturia inaequalis , the apple scab pathogen[22].…”

Section: Discussionmentioning

confidence: 99%

Characterization ofBacillusstrains from apple and pear trees in South Africa antagonistic toErwinia amylovora

Jock

Völksch

Mansvelt

et al. 2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

In order to find reasons for the absence of fire blight in most countries of the Southern hemisphere, bark samples from apple and pear trees in orchards of the Western Cape region in South Africa were extracted for bacteria which could be antagonistic to Erwinia amylovora. Screening was done in the late growth season and mainly Gram-positive bacteria were isolated. Approximately half of them produced growth inhibition zones on a lawn of E. amylovora. Most isolates were classified as Bacillus megaterium by microbiological assays and in API 50 test systems. They were visualized in the light microscope as non-motile large rods. These strains may not be responsible for the absence of fire blight in orchards, but they may indicate unfavourable climatic conditions for Gram-negative bacteria including E. amylovora. They may reduce the ability of E. amylovora to establish fire blight and could also be useful for application in biological disease control.

show abstract

“…According to the studies of Huang et al (2005) and Chiou and Wu (2001), several antagonistic bacteria were selected and examined for their biocontrol activities. Several studies reported biocontrol activity of B. pumilus strains against plant fungal pathogens (Arias et al 1999). In this study, chitinolytic B. pumilus SG2 was selected and its potential biocontrol activity was demonstrated by a dual culture assay.…”

Section: Discussionmentioning

confidence: 99%

Bacillus pumilus SG2 chitinases induced and regulated by chitin, show inhibitory activity against Fusarium graminearum and Bipolaris sorokiniana

et al. 2010

View full text Add to dashboard Cite

The possible role of chitinase in in vitro growth inhibition of the wheat pathogens Fusarium graminearum and Bipolaris sorokiniana by Bacillus pumilus SG2 was investigated. B. pumilus SG2, a chitinolytic bacterium producing two different chitinases, was previously isolated from the saline deserts of Iran. When grown in Spizizen salts medium with colloidal chitin, B. pumilus SG2 secreted two chitinases into the medium, resulting in growth inhibition of F. graminearum and abortion of hyphal elongation of B. sorokiniana. In contrast, when glucose was used as the carbon source, the chitinases were not expressed and antifungal activity of the B. pumilus SG2 was completely abolished. These results confirmed that expression of the B. pumilus SG2 chitinases is under the control of two types of regulation, special regulation by chitin and global regulation by glucose. We demonstrated that chitinases are the main components that caused hyphal inhibition activity of B. pumilus SG2. Hyphal inhibition of F. graminearum and B. sorokiniana was stable in agar for a minimum of 14 days.

show abstract

Spatio-temporal distribution of naturally occurringBacillus spp. and other bacteria on the phylloplane of soybean under field conditions

Cited by 38 publications

References 7 publications

Effect of Solar UV-B Radiation on a Phyllosphere Bacterial Community

Effect of Solar UV-B Radiation on a Phyllosphere Bacterial Community

Characterization ofBacillusstrains from apple and pear trees in South Africa antagonistic toErwinia amylovora

Bacillus pumilus SG2 chitinases induced and regulated by chitin, show inhibitory activity against Fusarium graminearum and Bipolaris sorokiniana

Contact Info

Product

Resources

About