Spatio-temporal diversity of free-living and particle-attached prokaryotes in the tropical lagoon of Ahe atoll (Tuamotu Archipelago) and its surrounding oceanic waters

Michotey, Valérie; Guasco, Sophie; Bœuf, Dominique Le; Morezzi, N.; Durieux, B.; Charpy, Loïc; Bonin, Patricia

doi:10.1016/j.marpolbul.2012.01.009

Cited by 25 publications

(7 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Archaea at PHF also includes phylotypes assigned to Thaumarchaeota and to Euryarchaeota Marine Group II which is not surprising because these two groups are the most abundant and frequently detected planktonic archaea (Delong, 2003 ; Galand et al, 2009 ) in various oceanic provinces (see the example of a tropical atoll, Michotey et al, 2012 ). Thaumarchaeota detected in PHF were particularly abundant in 2010 (70.6%) but less in 2011 (12.2%) clone libraries and showed high intra-phylum diversity with representatives of both the Cenarchaeales and the Nitrososphaerales orders (Supplementary Figure 4 ).…”

Section: Resultsmentioning

confidence: 98%

Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period

et al. 2015

View full text Add to dashboard Cite

Active carbonate chimneys from the shallow marine serpentinizing Prony Hydrothermal Field were sampled 3 times over a 6 years period at site ST09. Archaeal and bacterial communities composition was investigated using PCR-based methods (clone libraries, Denaturating Gel Gradient Electrophoresis, quantitative PCR) targeting 16S rRNA genes, methyl coenzyme M reductase A and dissimilatory sulfite reductase subunit B genes. Methanosarcinales (Euryarchaeota) and Thaumarchaea were the main archaeal members. The Methanosarcinales, also observed by epifluorescent microscopy and FISH, consisted of two phylotypes that were previously solely detected in two other serpentinitzing ecosystems (The Cedars and Lost City Hydrothermal Field). Surprisingly, members of the hyperthermophilic order Thermococcales were also found which may indicate the presence of a hot subsurface biosphere. The bacterial community mainly consisted of Firmicutes, Chloroflexi, Alpha-, Gamma-, Beta-, and Delta-proteobacteria and of the candidate division NPL-UPA2. Members of these taxa were consistently found each year and may therefore represent a stable core of the indigenous bacterial community of the PHF chimneys. Firmicutes isolates representing new bacterial taxa were obtained by cultivation under anaerobic conditions. Our study revealed diverse microbial communities in PHF ST09 related to methane and sulfur compounds that share common populations with other terrestrial or submarine serpentinizing ecosystems.

show abstract

Section: Resultsmentioning

confidence: 98%

Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Tropical and subtropical lagoons are closed systems more often than temperate coastal areas, but as of yet there are no studies using high-throughput sequencing published to our knowledge. By way of comparison, clone library studies, which target the most abundant taxa, have shown that the degree of similarity between the attached and free-living communities in tropical and subtropical lagoons is linked to seasonal patterns, with greater differences during the dry season in tropical regions (63) and greater differences during the rainy season in the subtropical lagoons (25).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Differences in Attached and Free-Living Bacterial Communities in a Temperate Coastal Lagoon during Summer, Revealed via High-Throughput 16S rRNA Gene Sequencing

Mohit

Archambault

Toupoint

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

bMost of what is known about coastal free-living and attached bacterial diversity is based on open coasts, with high particulate and nutrient riverine supply, terrestrial runoffs, and anthropogenic activities. The Magdalen Islands in the Gulf of St. Lawrence (Canada) are dominated by shallow lagoons with small, relatively pristine catchments and no freshwater input apart from rain. Such conditions provided an opportunity to investigate coastal free-living and attached marine bacterial diversity in the absence of confounding effects of steep freshwater gradients. We found significant differences between the two communities and marked temporal patterns in both. Taxonomic richness and diversity were greater in the attached than in the free-living community, increasing over summer, especially within the least abundant bacterial phyla. The highest number of reads fell within the SAR 11 clade (Pelagibacter, Alphaproteobacteria), which dominated free-living communities. The attached communities had deeper phylum-level diversity than the free-living fraction. Distance-based redundancy analysis indicated that the particulate organic matter (POM) concentration was the main variable separating early and late summer samples with salinity and temperature changes also significantly correlated to bacterial community structure. Our approach using high-throughput sequencing detected differences in free-living versus attached bacteria in the absence of riverine input, in keeping with the concept that marine attached communities are distinct from cooccurring free-living taxa. This diversity likely reflects the diverse microhabitats of available particles, implying that the total bacterial diversity in coastal systems is linked to particle supply and variability, with implications for understanding microbial biodiversity in marine systems.

show abstract

“…In the same lagoon, Loret et al (2000) showed that hetero/mixotrophic protists processed the picoplanktonic resource rapidly and efficiently for filter-feeders, particularly pearl oysters. In the Ahe lagoon, the importance of the microbial loop is supported by the study of Michotey et al (2012) showing spatiotemporal pervasiveness for heterotrophic groups such as Marinovum, Flavobacteria and Erytrobacter. The trophic link between metazooplankton and the microbial loop is suggested by our positive correlations between ciliates and total zooplankton (r = 0.82, p = 0.01) and by-the PCA for the functional groups (Fig.…”

Section: Relationships Between Zooplankton and Environmental And Tropmentioning

confidence: 97%

Metazooplankton communities in the Ahe atoll lagoon (Tuamotu Archipelago, French Polynesia): Spatiotemporal variations and trophic relationships

Pagano

Sagarra

Champalbert

et al. 2012

Marine Pollution Bulletin

View full text Add to dashboard Cite

Metazooplankton abundance, biomass (<80 μm, 200-500 μm and >500 μm) and community structure in the Ahe atoll were studied together with their relationships with environmental factors (temperature, salinity, wind) and trophic factors (phytoplankton, bacteria, heterotrophic nanoflagellates (HNF) and ciliates) during three periods in 2008-2009. Meroplankton, mainly bivalve and gastropod larvae, was dominant. Holoplankton was dominated by copepods, the main species being Oithona spp., Paracalanus parvus, Clausocalanus spp., Corycaeus spp., Acartia fossae and Undinula vulgaris. The results suggest a clear wind influence on the structure and horizontal distribution of the zooplankton communities. The metazooplankton appeared to be controlled mainly by food resources, suggesting a bottom-up control. The low nanophytoplankton biomass in contrast to the high abundance of picophytoplankton, HNF and nano-particle grazers (mainly Oithona spp., Paracalanus and bivalve larvae) highlighted the importance of the microbial loop in the food web.

show abstract

Spatio-temporal diversity of free-living and particle-attached prokaryotes in the tropical lagoon of Ahe atoll (Tuamotu Archipelago) and its surrounding oceanic waters

Cited by 25 publications

References 88 publications

Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period

Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period

Phylogenetic Differences in Attached and Free-Living Bacterial Communities in a Temperate Coastal Lagoon during Summer, Revealed via High-Throughput 16S rRNA Gene Sequencing

Metazooplankton communities in the Ahe atoll lagoon (Tuamotu Archipelago, French Polynesia): Spatiotemporal variations and trophic relationships

Contact Info

Product

Resources

About