Spatio-temporal expression of chromogranin A during zebrafish embryogenesis

Xie, Jing‐Tian; Wang, Weiqing; Liu, Tingxi; Deng, Min; Ning, Guang

doi:10.1677/joe-08-0221

Cited by 14 publications

(7 citation statements)

References 24 publications

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“…Our observations in chick reveal that there is a considerable conservation of the expression domains of these genes with other vertebrates, suggesting there may also be an evolutionary conservation of function. For example, Chga expression in zebrafish is described in the developing cranial ganglia and other NCC-derived structures leading the authors to hypothesize that Chga -expressing cells are solely derived from NCCs (Xie et al, 2008 ). The in situ hybridization data presented here essentially does not differ from the pattern reported for the zebrafish but several other expression areas are described, in particular in non-NCC-derived.…”

Section: Discussionmentioning

confidence: 99%

Dynamic expression of Notch-dependent neurogenic markers in the chick embryonic nervous system

et al. 2014

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The establishment of a functional nervous system requires a highly orchestrated process of neural proliferation and differentiation. The evolutionary conserved Notch signaling pathway is a key regulator of this process, regulating basic helix-loop-helix (bHLH) transcriptional repressors and proneural genes. However, little is known about downstream Notch targets and subsequently genes required for neuronal specification. In this report, the expression pattern of Transgelin 3 (Tagln3), Chromogranin A (Chga) and Contactin 2 (Cntn2) was described in detail during early chick embryogenesis. Expression of these genes was largely restricted to the nervous system including the early axon scaffold populations, cranial ganglia and spinal motor neurons. Their temporal and spatial expression were compared with the neuronal markers Nescient Helix-Loop-Helix 1 (Nhlh1), Stathmin 2 (Stmn2) and HuC/D. We show that Tagln3 is an early marker for post-mitotic neurons whereas Chga and Cntn2 are expressed in mature neurons. We demonstrate that inhibition of Notch signaling during spinal cord neurogenesis enhances expression of these markers. This data demonstrates that Tagln3, Chga and Cntn2 represent strong new candidates to contribute to the sequential progression of vertebrate neurogenesis.

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Section: Discussionmentioning

confidence: 99%

Dynamic expression of Notch-dependent neurogenic markers in the chick embryonic nervous system

et al. 2014

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“…Moreover, prior studies have shown that longer fixation in formalin reduces IHC staining. 14 Assessing the effect of different decalcification periods for the NBF groups, the longer decalcification protocol (7 days in CalExII) resulted in significantly increased average staining intensity scores for the N2w group, which is contrary to the expected effect. Similar to the lack of consistent trends with time spent in fixation, this may be related to interobserver variation.…”

Section: Decalcification Effect On Immunohistochemistrymentioning

confidence: 92%

Evaluating the Effects of Various Decalcification Protocols on Immunohistochemical Staining in Zebrafish (Danio rerio)

et al. 2019

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Fixation and decalcification can alter protein structure in tissues, influencing the efficacy of primary antibodies routinely used in immunohistochemical (IHC) staining. Histologic examination of zebrafish requires both processes, making staining and analysis potentially challenging. Here, we investigated the effects of common fixation and decalcification protocols on IHC staining in zebrafish. We also identified zebrafish-reactive andspecific antibodies for use in research and diagnostics. For several of the antibodies, time spent in Dietrich's fixative containing 2% glacial acetic acid or 3.4% formaldehyde followed by decalcification with ethylenediaminetetraacetic acid (EDTA) significantly impacted IHC staining quality, particularly regarding staining intensity. Protocols utilizing shorter fixation times produced higher-quality stains. In addition, individual markers were variably affected by the type of fixative. Dietrich's fixative significantly reduced staining quality for the ''neural'' markers: glial fibrillar acidic protein, chromogranin A, S100. A negative time-dependent effect of fixation on staining quality was found for several antibodies: muscle actin (Dietrich's only), cytokeratin AE1/ AE3, chromogranin, and S100. Neither decalcification protocol had a statistically significant negative timedependent effect on staining quality. Based on our results, we suggest shorter fixation and decalcification protocols to best preserve IHC staining quality as well as recommend deliberate selection of the fixative used depending on the protein of interest.

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“…Tagln3 is also expressed in the ventral MLF population, which are the first neurons to develop in the brain (Figure 3C , asterisk). While these target genes are all expressed in post-mitotic neurons (Theodorakis et al, 2002 ; Pape et al, 2008 ; Xie et al, 2008 ; Burzynski et al, 2009 ; Ratié et al, under review) no specific function can be attributed to these genes during nTPOC and nMTT development.…”

Section: Description Of Proneural Target Genes Within the Hypothalamimentioning

confidence: 99%

Notch signaling and proneural genes work together to control the neural building blocks for the initial scaffold in the hypothalamus

Ware

Hamdi-Rozé

Dupé³

2014

Front. Neuroanat.

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The vertebrate embryonic prosencephalon gives rise to the hypothalamus, which plays essential roles in sensory information processing as well as control of physiological homeostasis and behavior. While patterning of the hypothalamus has received much attention, initial neurogenesis in the developing hypothalamus has mostly been neglected. The first differentiating progenitor cells of the hypothalamus will give rise to neurons that form the nucleus of the tract of the postoptic commissure (nTPOC) and the nucleus of the mammillotegmental tract (nMTT). The formation of these neuronal populations has to be highly controlled both spatially and temporally as these tracts will form part of the ventral longitudinal tract (VLT) and act as a scaffold for later, follower axons. This review will cumulate and summarize the existing data available describing initial neurogenesis in the vertebrate hypothalamus. It is well-known that the Notch signaling pathway through the inhibition of proneural genes is a key regulator of neurogenesis in the vertebrate central nervous system. It has only recently been proposed that loss of Notch signaling in the developing chick embryo causes an increase in the number of neurons in the hypothalamus, highlighting an early function of the Notch pathway during hypothalamus formation. Further analysis in the chick and mouse hypothalamus confirms the expression of Notch components and Ascl1 before the appearance of the first differentiated neurons. Many newly identified proneural target genes were also found to be expressed during neuronal differentiation in the hypothalamus. Given the critical role that hypothalamic neural circuitry plays in maintaining homeostasis, it is particularly important to establish the targets downstream of this Notch/proneural network.

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Spatio-temporal expression of chromogranin A during zebrafish embryogenesis

Cited by 14 publications

References 24 publications

Dynamic expression of Notch-dependent neurogenic markers in the chick embryonic nervous system

Dynamic expression of Notch-dependent neurogenic markers in the chick embryonic nervous system

Evaluating the Effects of Various Decalcification Protocols on Immunohistochemical Staining in Zebrafish (Danio rerio)

Notch signaling and proneural genes work together to control the neural building blocks for the initial scaffold in the hypothalamus

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