Many Chinese cities currently are facing increased urban fire risks particularly at places such as urban villages, high-rise buildings and large warehouses. Using a unique historical fire incident dataset (2002–2013), this paper is intended to explore the urban fire dynamics and its association with urban growth in Nanjing, China, with a geographical information system (GIS)-based spatial analytics and remote sensing (RS) techniques. A new method is proposed to define a range of fire hot spots characterizing different phases of fire incident evolution, which are compared with the urban growth in the same periods. The results suggest that the fire events have been largely concentrated in the city proper and meanwhile expanding towards the suburbs, which has a similar temporal trend to the growth of population and urban land at the city level particularly since 2008. Most intensifying and persistent fire hot spots are found in the central districts, which have limited urban expansion but high population densities. Most new hot spots are located in the suburban districts, which have seen both rapid population growth and urban expansion in recent years. However, the analysis at a finer spatial scale (500 m × 500 m) shows no evidences of an explicit connection between the locations of new fire hot spots and recently developed urban land. The findings can inform future urban and emergency planning with respect to the deployment of fire and rescue resources, ultimately improving urban fire safety.