Spatio-temporally expressed sorbitol transporters cooperatively regulate sorbitol accumulation in pear fruit

Gu, Chao; Wu, Ruofan; Yu, Cai-Yun; Qi, Kaijie; Wu, Chen; Zhang, Shaoling; Zhang, Shaoling

doi:10.1016/j.plantsci.2020.110787

Cited by 17 publications

(11 citation statements)

References 68 publications

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“…This analysis confirmed the presence of all previously identified SOTs in pear and strawberry, aligning with prior research. For peach, we identified 11 SOT sequences, nine of which were in agreement with earlier findings (Gu et al., 2021; Yu et al., 2019). However, we noticed discrepancies in apple (Golden Delicious) sequence screening when compared to previous results.…”

Section: Discussionsupporting

confidence: 92%

“…Efficient and controlled distribution of sugar from source to sink is a crucial determinant of plant growth and development (Wang et al., 2013). While sucrose serves as the primary transport product of photosynthesis in most fruit trees, synthesized in source tissues and transported to reservoir tissues through a switched phloem‐unloading pathway from symplasmic‐to‐apoplasmic (Gu et al., 2021; Slawinski et al., 2021), in the case of Rosaceae species, the main photosynthate and soluble storage substance is sorbitol, which is distributed via sorbitol transporter (SOTs). SOTs are recognized as crucial components of the Polyol/Monosaccharide Transporter (PMTs or PLTs) family.…”

Section: Introductionmentioning

confidence: 99%

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Origin and early divergence of tandem duplicated sorbitol transporter genes in Rosaceae: insights from evolutionary analysis of the SOT gene family in angiosperms

Yang,

Luo,

Guo

et al. 2023

The Plant Journal

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SUMMARYSorbitol is a critical photosynthate and storage substance in the Rosaceae family. Sorbitol transporters (SOTs) play a vital role in facilitating sorbitol allocation from source to sink organs and sugar accumulation in sink organs. While prior research has addressed gene duplications within the SOT gene family in Rosaceae, the precise origin and evolutionary dynamics of these duplications remain unclear, largely due to the complicated interplay of whole genome duplications and tandem duplications. Here, we investigated the synteny relationships among all identified Polyol/Monosaccharide Transporter (PLT) genes in 61 angiosperm genomes and SOT genes in representative genomes within the Rosaceae family. By integrating phylogenetic analyses, we elucidated the lineage‐specific expansion and syntenic conservation of PLTs and SOTs across diverse plant lineages. We found that Rosaceae SOTs, as PLT family members, originated from a pair of tandemly duplicated PLT genes within Class III‐A. Furthermore, our investigation highlights the role of lineage‐specific and synergistic duplications in Amygdaloideae in contributing to the expansion of SOTs in Rosaceae plants. Collectively, our findings provide insights into the genomic origins, duplication events, and subsequent divergence of SOT gene family members. Such insights lay a crucial foundation for comprehensive functional characterizations in future studies.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Origin and early divergence of tandem duplicated sorbitol transporter genes in Rosaceae: insights from evolutionary analysis of the SOT gene family in angiosperms

Yang,

Luo,

Guo

et al. 2023

The Plant Journal

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“…Moreover, the higher content of sorbitol also plays a pivotal role in fruit quality and senescence. Gu et al 49 found that abscisic acid or indole acetic acid treatment could promote sorbitol accumulation and improve the fruit quality through mediating sorbitol transporters in pear fruit. Furthermore, sorbitol content not only affects the fruit quality but also relates to the disease resistance of the fruit.…”

Section: ■ Discussionmentioning

confidence: 99%

Trehalose Regulates Starch, Sorbitol, and Energy Metabolism to Enhance Tolerance to Blue Mold of “Golden Delicious” Apple Fruit

Sun

Zhu

et al. 2022

J. Agric. Food Chem.

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The efficacy of trehalose on the lesion diameter of apples (cv. Golden Delicious) inoculated with Penicillium expansum was evaluated to screen the optimal concentration. The changes in gene expression and activity of the enzyme in starch, sorbitol, and energy metabolism were also investigated in apples after trehalose treatment. The results revealed that trehalose dipping reduced the lesion diameter of apples inoculated with P. expansum. Trehalose suppressed the activities and gene expressions of β-amylase, NAD-sorbitol dehydrogenase, and NADP-sorbitol dehydrogenase, whereas it decreased the sorbitol 6-phosphate dehydrogenase gene expression and amylose, amylopectin, total starch, and reducing sugar contents. Additionally, trehalose improved the gene expressions and activities of α-amylase, starch-branching enzymes, total amylase, H+-ATPase, and Ca2+-ATPase, as well as soluble sugar, adenosine triphosphate, and adenosine diphosphate contents and energy charge in apples. These findings imply that trehalose could induce tolerance to the blue mold of apple fruit by regulating starch, sorbitol, and energy metabolism.

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“…The recombinant vector, along with pTRV1, was then individually introduced into the A. tumefaciens strain GV3101. Afterward, they were combined in a 1:1 ratio before injection into 'Cuiguan' fruit at 100 DAFB, as described by Gu et al (2021). Five days after injection, cortex samples were collected from the injection sites.…”

Section: Transient Silencing Of Genes In Pear Fruitmentioning

confidence: 99%

PbrbZIP15 promotes sugar accumulation in pear via activating the transcription of the glucose isomerase gene PbrXylA1

Jia,

Zhang,

Zhang

et al. 2023

The Plant Journal

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SUMMARYThe composition and abundance of soluble sugars in mature pear (Pyrus) fruit are important for its acceptance by consumers. However, our understanding of the genes responsible for soluble sugar accumulation remains limited. In this study, a S1‐group member of bZIP gene family, PbrbZIP15, was characterized from pear genome through the combined analyses of metabolite and transcriptome data followed by experimental validation. PbrbZIP15, located in nucleus, was found to function in fructose, sucrose, and total soluble sugar accumulation in pear fruit and calli. After analyzing the expression profiles of sugar‐metabolism‐related genes and the distribution of cis‐acting elements in their promoters, the glucose isomerase 1 gene (PbrXylA1), whose corresponding protein catalyzed the isomerization of glucose and fructose in vitro, was identified as a downstream target gene of PbrbZIP15. PbrbZIP15 could directly bind to the G‐box element in PbrXylA1 promoter and activate its transcription, as evidenced by chromatin immunoprecipitation‐quantitative PCR, yeast one‐hybrid, electrophoretic mobility shift assay, and dual‐luciferase assay. PbrXylA1, featuring a leucine‐rich signal peptide in its N‐terminal, was localized to the endoplasmic reticulum. It was validated to play a significant role in fructose, sucrose, and total soluble sugar accumulation in pear fruit and calli, which was associated with the upregulated fructose/glucose ratio. Further studies revealed a positive correlation between the sucrose content and the expression levels of several sucrose‐biosynthesis‐related genes (PbrFRK3/8, PbrSPS1/3/4/8, and PbrSPP1) in PbrbZIP15‐/PbrXylA1‐transgenic fruit/calli. In conclusion, our results suggest that PbrbZIP15‐induced soluble sugar accumulation during pear development is at least partly attributed to the activation of PbrXylA1 transcription.

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Spatio-temporally expressed sorbitol transporters cooperatively regulate sorbitol accumulation in pear fruit

Cited by 17 publications

References 68 publications

Origin and early divergence of tandem duplicated sorbitol transporter genes in Rosaceae: insights from evolutionary analysis of the SOT gene family in angiosperms

Origin and early divergence of tandem duplicated sorbitol transporter genes in Rosaceae: insights from evolutionary analysis of the SOT gene family in angiosperms

Trehalose Regulates Starch, Sorbitol, and Energy Metabolism to Enhance Tolerance to Blue Mold of “Golden Delicious” Apple Fruit

PbrbZIP15 promotes sugar accumulation in pear via activating the transcription of the glucose isomerase gene PbrXylA1

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