Spatio-Temporally Restricted Expression of Cell Adhesion Molecules during Chicken Embryonic Development

Abstract

Differential cell adhesive properties are known to regulate important developmental events like cell sorting and cell migration. Cadherins and protocadherins are known to mediate these cellular properties. Though a large number of such molecules have been predicted, their characterization in terms of interactive properties and cellular roles is far from being comprehensive. To narrow down the tissue context and collect correlative evidence for tissue specific roles of these molecules, we have carried out whole-mount in situ hybridization based RNA expression study for seven cadherins and four protocadherins. In developing chicken embryos (HH stages 18, 22, 26 and 28) cadherins and protocadherins are expressed in tissue restricted manner. This expression study elucidates precise expression domains of cell adhesion molecules in the context of developing embryos. These expression domains provide spatio-temporal context in which the function of these genes can be further explored.

Exploring the chicken embryo as a possible model for studying Listeria monocytogenes pathogenicity

“…This is in contrast to mice which harbor a glutamic acid at position 16 of E-cadherin, preventing a successful interaction and hence invasion. A recent study could give a possible explanation of our results (Roy and Bandyopadhyay, 2014 ). In that work, the authors showed that the expression of the mRNA encoding E-cadherin was high early during the chicken development in all examined tissues, but diminished in chicken embryos older than 6 days.…”

Family-wide Structural and Biophysical Analysis of Binding Interactions among Non-clustered δ-Protocadherins

“…The remarkable cell patterns that arise in GCE through X-linked inheritance of inactive forms of the Pcdh19 gene leaves little doubt that it plays an important role in cell adhesion (Pederick et al, 2018). d-protocadherins also display expression patterns that differentially track tissue structures (Etzrodt et al, 2009;Redies et al, 2005), and for which changes in expression often precede the emergence of anatomical features in development Roy and Bandyopadhyay, 2014). Phenotypes potentially related to adhesion have been found for numerous other d-protocadherins, as well (Bononi et al, 2008;Cooper et al, 2015;Hayashi et al, 2014;Hayashi and Takeichi, 2015;Hoshina et al, 2013;Uemura et al, 2007;Zhu et al, 2014).…”

The Transcription Factor-microRNA Regulatory Network during hESC-chondrogenesis

“…A network of genes linked to IGF1, a positive regulator of cartilage ECM [49][50][51][52] , was identified within this network, including IGFBP3. Transcripts for the cell adhesion molecules CDH5,11 and 13, identified by STRING analysis, are also known to be expressed in the chick limb bud with CDH11 present from an early stage 53 . It is likely that the potassium voltage gated ion channels identified (KCNQ5, KCNA3, KCNE4, KCND3) may similarly be involved in ion balance and mechano-regulation.…”