Spatiotemporal analysis of bacterial biomass and activity to understand surface and groundwater interactions in a highly dynamic riverbank filtration system

Driezum, Inge H. van; Chik, Alex H.S.; Jakwerth, Stefan; Lindner, Gerhard; Farnleitner, Andreas H.; Sommer, Regina; Blaschke, Alfred Paul; Kirschner, Alexander K. T.

doi:10.1016/j.scitotenv.2018.01.226

Cited by 32 publications

(18 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Absolute quantification of relative abundances gives a surplus value on the generated information on microbial dynamics such that conclusions can be drawn on actual shifts in microbial populations ( Props et al, 2017 ). In general, mixing surface water with groundwater does increase cell counts due to cell introduction from surface water, the stimulation of heterotrophic respiration, as well as altered organic carbon compositions and is therefore used to monitor microbial dynamics ( Stegen et al, 2016 ; Epting et al, 2018 ; van Driezum et al, 2018 ). The impression of suppressing microbial phyla due to the increase of others suggests that allochthonous microorganisms inhabit ecological niches instead of autochthonous ones ( Props et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Microbial Community Dynamics in River Bank Filtrate Using High-Throughput Sequencing and Flow Cytometry

et al. 2018

View full text Add to dashboard Cite

Surface-groundwater interactions play an important role in microbial community compositions of river bank filtrates. Surface water contaminations deriving from environmental influences are attenuated by biogeochemical processes in the hyporheic zone, which are essential for providing clean and high-quality drinking water in abstraction wells. Characterizing the flow regime of surface water into the groundwater body can provide substantial information on water quality, but complex hydraulic dynamics make predictions difficult. Thus, a bottom up approach using microbial community shifting patterns as an overall outcome of dynamic water characteristics could provide more detailed information on the influences that affect groundwater quality. The combination of high-throughput sequencing data together with flow cytometric measurements of total cell counts reveals absolute abundances among taxa, thus enhancing interpretation of bacterial dynamics. 16S rRNA high-throughput sequencing of 55 samples among six wells in a well field in Austria that is influenced by river bank filtrate within a time period of 3 months has revealed both, clear differences as well as strong similarity in microbiome compositions between wells and dates. A significant community shift from April to May occurred in four of six wells, suggesting that surface water flow regimes do affect these wells stronger than others. Triplicate sampling and subsequent sequencing of wells at different dates proved the method to be reproducible. Flow cytometric measurements of total cells indicate microbial shifts due to increased cell counts and emphasize the rise of allochthonous microorganisms. Typical freshwater bacterial lineages (Verrucomicrobia, Bacteroidetes, Actinobacteria, Cyanobacteria, Armatimonadetes) were identified as most increasing phyla during community shifts. The changes are most likely a result of increased water abstraction in the wells together with constant river water levels rather than rain events. The results provide important knowledge for future implementations of well utilization in dependency of the nearby Danube River water levels and can help drawing conclusions about the influence of surface water in the groundwater such that hygienically save and clean drinking water with a stable microbial community can be provided.

show abstract

Section: Discussionmentioning

confidence: 99%

Assessment of Microbial Community Dynamics in River Bank Filtrate Using High-Throughput Sequencing and Flow Cytometry

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Samples from the River Leith, NW England, in an area featuring groundwater-surface interaction showed a slightly lower TLF loss following filtration of 20-40% 27 , albeit through a larger pore-size 0.45 µm membrane that would not remove all microbes. In groundwater, we might expect an even higher proportion of TLF and HLF to be extracellular due to natural filtration during recharge and subsurface flow, as well as, typically, a lower microbial biomass [28][29][30][31] . This expectation would have implications for TLF/ HLF use as faecal indicators in groundwater, which provides the majority of the global drinking water supply.…”

mentioning

confidence: 99%

Tryptophan-like and humic-like fluorophores are extracellular in groundwater: implications as real-time faecal indicators

Sorensen

Carr

Nayebare

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Fluorescent natural organic matter at tryptophan-like (TLF) and humic-like fluorescence (HLF) peaks is associated with the presence and enumeration of faecal indicator bacteria in groundwater. We hypothesise, however, that it is predominantly extracellular material that fluoresces at these wavelengths, not bacterial cells. We quantified total (unfiltered) and extracellular (filtered at < 0.22 µm) TLF and HLF in 140 groundwater sources across a range of urban population densities in Kenya, Malawi, Senegal, and Uganda. Where changes in fluorescence occurred following filtration they were correlated with potential controlling variables. A significant reduction in TLF following filtration (ΔTLF) was observed across the entire dataset, although the majority of the signal remained and thus considered extracellular (median 96.9%). ΔTLF was only significant in more urbanised study areas where TLF was greatest. Beneath Dakar, Senegal, ΔTLF was significantly correlated to total bacterial cells (ρs 0.51). No significant change in HLF following filtration across all data indicates these fluorophores are extracellular. Our results suggest that TLF and HLF are more mobile than faecal indicator bacteria and larger pathogens in groundwater, as the predominantly extracellular fluorophores are less prone to straining. Consequently, TLF/HLF are more precautionary indicators of microbial risks than faecal indicator bacteria in groundwater-derived drinking water.

show abstract

“…Less attention has been paid to groundwater‐level oscillations driven by RI. In contrast, RI is characterized by downward flow of O 2 –rich rainfall water that can entrap soil pore air in vadose and oscillated zones, potentially elevating O 2 level in the saturated zone (Neale, Hughes, & Ward, 2000; Van Driezum et al., 2018). Hence, O 2 level along with soil moisture content (or water saturation) will exhibit different temporal patterns within the vadose, oscillated, and saturated zones of an aquifer in response to NF and RI.…”

Section: Introductionmentioning

confidence: 99%

Response of soil bacterial community and geochemical parameters to cyclic groundwater‐level oscillations in laboratory columns

Xia

Cheng

Zhu

et al. 2020

Vadose Zone Journal

View full text Add to dashboard Cite

Groundwater-level oscillations change geochemical conditions, C cycling processes, and bacterial community composition, and these changes may vary vertically with depth in a soil. In this study, soil column experiments were conducted to explore variations in soil bacterial community composition and its associated geochemical parameters to rapid short-term cyclic groundwater-level oscillations driven by natural fluctuations (NF) and rainfall infiltration (RI), and the results are compared with a quasistatic (QS) column. Water saturation patterns in vadose and oscillated zones, and O 2 level patterns, soil total organic C (TOC) removal rates, and soil bacterial community composition in vadose, oscillated, and saturated zones were evaluated. Results showed that water saturation and O 2 level oscillated with groundwater level in NF and RI columns. Total organic C removal rates in RI column were the highest across vadose (∼38.4%), oscillated (∼35.8%), and saturated (∼35.2%) zones. Deltaproteobacteria, which were significantly correlated with TOC removal (p < .05), were found in higher abundance in the vadose and oscillated zones of the RI column than in the QS and NF columns. Soil bacterial community structure was dynamic at the class level due to water saturation, O 2 level, and TOC removal. Total organic C removal was the driver to separate the distribution of bacterial community structure in the vadose and oscillated zones of the RI column from those of the QS and NF columns. This study suggests that RI induced rapid, short-term, cyclic, groundwater-level oscillations could significantly influence both the soil C cycle and bacterial community structure in the vadose and oscillated zones.

show abstract

Spatiotemporal analysis of bacterial biomass and activity to understand surface and groundwater interactions in a highly dynamic riverbank filtration system

Cited by 32 publications

References 40 publications

Assessment of Microbial Community Dynamics in River Bank Filtrate Using High-Throughput Sequencing and Flow Cytometry

Assessment of Microbial Community Dynamics in River Bank Filtrate Using High-Throughput Sequencing and Flow Cytometry

Tryptophan-like and humic-like fluorophores are extracellular in groundwater: implications as real-time faecal indicators

Response of soil bacterial community and geochemical parameters to cyclic groundwater‐level oscillations in laboratory columns

Contact Info

Product

Resources

About