Urbanization-induced land use transitions (LUTs) result in a decline in ecosystem services, which has implications for regional ecological security. In order to explore the relationship between ecosystem services and land use transition, this paper utilizes the InVEST model, a geographically weighted regression (GWR) model, to examine the impact of land use transition on ecosystem services in the Dongting Lake area (DLA). The results showed that 1) with the change in urbanization development, the average values of land use transition intensity (LUI) in 2000, 2010, and 2020 are 237.99, 235.82, and 238.92, respectively. Land use dynamics (LUD) show a tendency to increase and then decrease, with average values of 5.58 and 5.62 for the periods 2000–2010 and 2010–2020, respectively, and the transformation of land use shows obvious spatio-temporal heterogeneity. 2) Habitat quality and carbon sequestration showed a downward trend. In contrast, food supply followed an upward trend; soil conservation (SC) and water yield (WY) services initially increased and decreased later. The overall spatial changes in habitat quality and carbon sequestration appear to be insignificant. Food supply shows significant differences in the plains compared to other areas, while soil conservation and water yield service show significant changes in places other than the DLA. 3) From 2000 to 2020, land use transition dynamics, population density, GDP density, night lighting, and transition intensity had mainly negative effects on ecosystem services. Only the Normalized Vegetation Index (NDVI) showed a positive effect on ecosystem services. The results of the research will provide valuable references for the development and implementation of spatial ecological restoration planning and land use policies in the national territory.