Spatiotemporal control of cell–cell reversible interactions using molecular engineering

Shi, Peng; Ju, Enguo; Yan, Zhengqing; Gao, Nan; Wang, Jiasi; Hou, Jianwen; Zhang, Yan; Ren, Jinsong; Qu, Xiaogang

doi:10.1038/ncomms13088

Cited by 104 publications

(114 citation statements)

References 44 publications

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“…A similar strategy was used to metabolically label cells with peracetylated N-azidoacetylgalactosamine (Ac 4 GalNAz), introducing azido groups into cell surface glycoconjugates (Shi et al, 2016). Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) was then used to install a pegylated β-cyclodextrin (β-CD).…”

Section: Non-genetic Approaches To Membrane Engineeringmentioning

confidence: 99%

“…PBMCs were labeled with Ac 4 GalNAz to yield cell surface azide groups that were subsequently conjugated to a β-CD. Azobenzene-conjugated anti-MUC1 aptamers were then installed on the PBMCs, enabling them to target the MUC 1+ breast adenocarcinoma MCF-7 cell line in vitro (Shi et al, 2016). …”

Section: Applications For Engineered Cell-cell Interactionsmentioning

confidence: 99%

“…To this end, many groups have incorporated temporospatial control mechanisms into their surface modification approaches. For example, the introduction of photoisomerizable (Shi et al, 2016) and photocleavable (Luo et al, 2014) groups into the modifications has allowing their removal upon UV irradiation. Electrochemical reversibility has also been demonstrated, with the formation and cleavage of oxime linkages reliant upon oxidation and reduction, respectively (Pulsipher et al, 2014).…”

Section: Perspectivementioning

confidence: 99%

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Programming Cell-Cell Interactions through Non-genetic Membrane Engineering

Csizmar

Petersburg

Wagner

2018

Cell Chemical Biology

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The ability to direct targeted intercellular interactions has the potential to enable and expand the use of cell-based therapies for regenerative medicine, tissue engineering, and immunotherapy. While genetic engineering approaches have proven effective, these techniques are not amenable to all cell types and often yield permanent modifications with potentially long-lasting adverse effects, restricting their application. To circumvent these limitations, there is intense interest in developing non-genetic methods to modify cell membranes with functional groups that will enable the recognition of target cells. While many such techniques have been developed, relatively few have been applied to directing specific cell-cell interactions. This review details these non-genetic membrane engineering approaches-namely, hydrophobic membrane insertion, chemical modification, liposome fusion, metabolic engineering, and enzymatic remodeling-and summarizes their major applications. Based on this analysis, perspective is provided on the ideal features of these systems with an emphasis on the potential for clinical translation.

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Section: Non-genetic Approaches To Membrane Engineeringmentioning

confidence: 99%

Section: Applications For Engineered Cell-cell Interactionsmentioning

confidence: 99%

Section: Perspectivementioning

confidence: 99%

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Programming Cell-Cell Interactions through Non-genetic Membrane Engineering

Csizmar

Petersburg

Wagner

2018

Cell Chemical Biology

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“…Molecular engineering has recently been applied to achieve reversible, light-controlled assembly of cell adhesions without directly targeting cadherin [65]. In this technique, β-cyclodextrin is incorporated into plasma membranes allowing cells to bind to azobenzene, a photo-switchable compound whose trans but not cis isomer binds β-cyclodextrin.…”

Section: Future Directions: Replicating Tissue Shape To Engineer Organsmentioning

confidence: 99%

“…In this technique, β-cyclodextrin is incorporated into plasma membranes allowing cells to bind to azobenzene, a photo-switchable compound whose trans but not cis isomer binds β-cyclodextrin. Using engineered molecules including two azobenzene components connected by a polyethylene glycol chain, nearby cells displaying β-cyclodextrin can be induced to bind each azobenzene in the presence of visual light [65]. This binding forces cells into close apposition, which may encourage the formation of cadherin-based adhesions.…”

Section: Future Directions: Replicating Tissue Shape To Engineer Organsmentioning

confidence: 99%

Generating tissue topology through remodeling of cell-cell adhesions

Goodwin

Nelson

2017

Experimental Cell Research

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During tissue morphogenesis, cellular rearrangements give rise to a large variety of three-dimensional structures. Final tissue architecture varies greatly across organs, and many develop to include combinations of folds, tubes, and branched networks. To achieve these different tissue geometries, constituent cells must follow different programs that dictate changes in shape and/or migratory behavior. One essential component of these changes is the remodeling of cell-cell adhesions. Invasive migratory behavior and separation between tissues require localized breakdown of cadherin-mediated adhesions. Conversely, tissue folding and fusion require the formation and reinforcement of cell-cell adhesions. Cell-cell adhesion plays a critical role in tissue morphogenesis; its manipulation may therefore prove to be invaluable in generating complex topologies ex vivo. Recapitulating these shapes in engineered tissues would enable a better understanding of how these processes occur in vivo, and may lead to improved design of organs for clinical applications. In this review, we discuss work investigating the formation of folds, tubes, and branched networks with an emphasis on known or possible roles for cell-cell adhesion. We then examine recently developed tools that could be adapted to manipulate cell-cell adhesion in engineered tissues.

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Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom‐Up Tissue Engineering

et al. 2019

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Controlling cell–cell interactions is central for understanding key cellular processes and bottom‐up tissue assembly from single cells. The challenge is to control cell–cell interactions dynamically and reversibly with high spatiotemporal precision noninvasively and sustainably. In this study, cell–cell interactions are controlled with visible light using an optogenetic approach by expressing the blue light switchable proteins CRY2 or CIBN on the surfaces of cells. CRY2 and CIBN expressing cells form specific heterophilic interactions under blue light providing precise control in space and time. Further, these interactions are reversible in the dark and can be repeatedly and dynamically switched on and off. Unlike previous approaches, these genetically encoded proteins allow for long‐term expression of the interaction domains and respond to nontoxic low intensity blue light. In addition, these interactions are suitable to assemble cells into 3D multicellular architectures. Overall, this approach captures the dynamic and reversible nature of cell–cell interactions and controls them noninvasively and sustainably both in space and time. This provides a new way of studying cell–cell interactions and assembling cellular building blocks into tissues with unmatched flexibility.

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Spatiotemporal control of cell–cell reversible interactions using molecular engineering

Cited by 104 publications

References 44 publications

Programming Cell-Cell Interactions through Non-genetic Membrane Engineering

Programming Cell-Cell Interactions through Non-genetic Membrane Engineering

Generating tissue topology through remodeling of cell-cell adhesions

Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom‐Up Tissue Engineering

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