Spatiotemporal Coordination of RPE Cell Quality by Extracellular Vesicle miR-494-3p Via Competitive Interplays With SIRT3 or PTEN

Hamuro, Junji; Yamashita, Tomoko; Otsuki, Yohei; Hiramoto, Nao; Adachi, Mayuka; Miyatani, Takafumi; Tanaka, Hiroshi; Ueno, Masaki; Kinoshita, Shigeru; Sotozono, Chie

doi:10.1167/iovs.64.5.9

Cited by 2 publications

(1 citation statement)

References 53 publications

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“…MMP detection [ 19 ]: Firstly, JC-1 was configured into a working solution with RPMI 1640 medium at a concentration of 4 μmol/L according to the instructions. Imaging Buffer (10×) was diluted 10-fold with ultrapure water.…”

Section: Methodsmentioning

confidence: 99%

Copper Sulfate Combined with Photodynamic Therapy Enhances Antifungal Effect by Downregulating AIF1

Zhang,

An,

Wang

et al. 2024

JoF

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Candida albicans is a clinically significant opportunistic fungus that is generally treated with antifungal drugs such as itraconazole and fluconazole. However, the recent emergence of fungal resistance has made treatment increasingly difficult. Therefore, novel antifungal treatment methods are urgently required. Hexanol ethosome photodynamic therapy (HE-PDT) is a method that uses photosensitizers (PS), such as hexanol ethosome, to exert antifungal effects, and can be used to treat resistant fungal strains. However, due to the high dose of PS required for antifungal treatment, excess photosensitizers may remain. Furthermore, once exposed to light, normal tissues or cells are damaged after photodynamic therapy, which limits the clinical application of HE-PDT. Therefore, improving the efficacy without increasing the dose is the key to this treatment. In this study, the antifungal effect of copper sulfate combined with HE-PDT was investigated, and its mechanism was explored. The results suggested that exogenous copper sulfate significantly increased the antifungal effect of HE-PDT by enhancing the rate of C. albicans inhibition, increasing reactive oxygen species (ROS) accumulation, increasing the rate of apoptosis, and altering the mitochondrial membrane potential (MMP) and ATP concentration, which is related to the downregulation of apoptosis-inducing factor (AIF1) expression. In conclusion, copper sulfate combined with photodynamic therapy significantly inhibited the activity of C. albicans by inducing apoptosis. The combined approach reported herein provides new insights for future antifungal therapy.

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Section: Methodsmentioning

confidence: 99%

Copper Sulfate Combined with Photodynamic Therapy Enhances Antifungal Effect by Downregulating AIF1

Zhang,

An,

Wang

et al. 2024

JoF

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Clustered ARPE-19 cells distinct in mitochondrial membrane potential may play a pivotal role in cell differentiation

Miyatani,

Tanaka,

Numa

et al. 2024

Sci Rep

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Age-related macular degeneration (AMD) is associated with the dysfunction and degeneration of retinal pigment epithelium (RPE) cells. Here, we examined how the formation and expansions of cell clusters are regulated by the differentiation of the RPE　cells. In this study, ARPE-19 cells were cultivated in standard or differentiation media, i.e., without or with nicotinamide, to evaluate the spreading of cell clusters specified with differentiated cell phenotypes. Mitochondria membrane potential (MMP) and the distribution of the RPE cell clusters was also monitored with or without rotenone, a mitochondrial electron transport chain (ETC) complex I inhibitor. Cultured ARPE-19 cells generated scattered cell clusters composed mostly of smaller size cells expressing the differentiation markers mouse anti-cellular retinaldehyde–binding protein (CRALBP) and Bestrophin only in differentiation medium. After the increase of the number of clusters, the clusters appeared to paracellularly merge, resulting in expansion of the area occupied by the clusters. Of note, the cells within the clusters selectively had high MMP and were in accordance with the expression of RPE differentiation markers. Rotenone repressed the formation of the clusters and decreased intracellular MMP. The above results suggest that clustering of RPE cells with functional mitochondria plays a pivotal role in RPE cell differentiation process and the ETC complex I inhibition greatly influences the composition of RPE cells that are degenerated or differentiation disposed.

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Spatiotemporal Coordination of RPE Cell Quality by Extracellular Vesicle miR-494-3p Via Competitive Interplays With SIRT3 or PTEN

Cited by 2 publications

References 53 publications

Copper Sulfate Combined with Photodynamic Therapy Enhances Antifungal Effect by Downregulating AIF1

Copper Sulfate Combined with Photodynamic Therapy Enhances Antifungal Effect by Downregulating AIF1

Clustered ARPE-19 cells distinct in mitochondrial membrane potential may play a pivotal role in cell differentiation

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