Spatiotemporal dynamics of SIRT 1, 2 and 3 during in vitro maturation of bovine oocytes

Ferreira, Ana Filipa; Machado-Simões, Juliana; Soares, Maria; Sousa, Ana Paula; Ramalho‐Santos, João; Almeida-Santos, Teresa

doi:10.1016/j.theriogenology.2022.04.004

Cited by 8 publications

(5 citation statements)

References 61 publications

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“…Recently, the detailed temporal and spatial localization of SIRT1 during the maturation of the bovine oocyte was presented. At the germinal vesicle (GV) stage, SIRT1 is mainly localized within the nucleus [ 31 ]. Shortly after meiotic division resumes at the GV breakdown (GVBD) stage and as meiotic division progresses, SIRT1 expression levels increase, contributing to the presence of SIRT1 throughout the ooplasm [ 31 ].…”

Section: Sirt1 and Quality And Competence Of Oocytesmentioning

confidence: 99%

“…At the germinal vesicle (GV) stage, SIRT1 is mainly localized within the nucleus [ 31 ]. Shortly after meiotic division resumes at the GV breakdown (GVBD) stage and as meiotic division progresses, SIRT1 expression levels increase, contributing to the presence of SIRT1 throughout the ooplasm [ 31 ]. Although changes in histone acetylation are constantly present during division, their intensity varies between phases, as shown in a mouse model [ 32 ].…”

Section: Sirt1 and Quality And Competence Of Oocytesmentioning

confidence: 99%

“…Although changes in histone acetylation are constantly present during division, their intensity varies between phases, as shown in a mouse model [ 32 ]. Of particular interest seems to be the effect of SIRT1 on the course of metaphase I and II [ 31 ]. Several works have documented the focus of SIRT1 on a-tubules in metaphase I and II in mice and domestic cattle [ 31 , 33 ].…”

Section: Sirt1 and Quality And Competence Of Oocytesmentioning

confidence: 99%

“…Of particular interest seems to be the effect of SIRT1 on the course of metaphase I and II [ 31 ]. Several works have documented the focus of SIRT1 on a-tubules in metaphase I and II in mice and domestic cattle [ 31 , 33 ]. Accordingly, SIRT1 is involved in meiotic spindle patterning by modifying the level of the histone acetylation ( Figure 2 ) [ 33 ].…”

Section: Sirt1 and Quality And Competence Of Oocytesmentioning

confidence: 99%

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Resveratrol and SIRT1: Antiaging Cornerstones for Oocytes?

Grzeczka

Kordowitzki

2022

Nutrients

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It is well-known that there is an enormous variability in the aging-related decline of oocytes’ quantity and their developmental competence among mammalian species. The implication of female germline aging is profound from the perspective of evolutionary conservation of the aging mechanism, a topic of continuous and widespread interest that has yet to be fully addressed for the mammalian oocyte. There is a certain need to develop novel antiaging strategies to delay or slow down aging, or even to reverse the aging phenotype in the oocyte. In the past two decades, several antioxidants have been tested for this purpose. Resveratrol is one of these latter-mentioned compounds, which has shown anti-inflammatory and antiaging properties in a dose-dependent manner. Interestingly, resveratrol appears to enhance the activity of so-called Sirtuin 1, too. Therefore, the aim of this review is to summarize and discuss the latest findings related to resveratrol, Sirtuin 1, and their crosstalk and influence on the mammalian oocyte to elucidate the question of whether these factors can delay or slow down reproductive aging.

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Section: Sirt1 and Quality And Competence Of Oocytesmentioning

confidence: 99%

Section: Sirt1 and Quality And Competence Of Oocytesmentioning

confidence: 99%

Section: Sirt1 and Quality And Competence Of Oocytesmentioning

confidence: 99%

Section: Sirt1 and Quality And Competence Of Oocytesmentioning

confidence: 99%

See 2 more Smart Citations

Resveratrol and SIRT1: Antiaging Cornerstones for Oocytes?

Grzeczka

Kordowitzki

2022

Nutrients

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“…Ageing in the follicular microenvironment is a pleiotropic phenomenon, and age-associated alterations in mitochondria number, morphology/ultrastructure [ 9 , 19 ], distribution [ 20 ], and functionality, including mitochondrial membrane potential (∆ψm), metabolism, steroidogenesis [ 9 , 21 , 22 , 23 ], mtDNA copy number [ 24 ], and mtDNA mutations, result in an overall reduced energy state and increased reactive oxygen species (ROS) production [ 17 ]. Reversing these effects could contribute towards the development of new therapeutic strategies capable of mitigating age-related oocyte mitochondrial deterioration.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?

Almeida-Reis,

Carvalho,

Dias

et al. 2024

Biomolecules

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Age-related changes in the mitochondrial status of human cumulus cells (hCCs) impact oocyte quality; however, the relationship between hCC mitochondrial (dys)function and reproductive aging remains poorly understood. This study aimed to establish the interplay between hCC mitochondrial dysfunction and women’s reproductive potential. In this investigation, 266 women were enrolled and categorized into two groups based on their age: a young group (<35 years old) and an advanced maternal age (AMA) group (≥35 years old). Comprehensive analysis of reproductive outcomes was conducted in our population. Various mitochondrial-related parameters were analyzed across distinct subsets. Specifically, mitochondrial membrane potential (∆Ψm) and mitochondrial mass were examined in 53 samples, mtDNA content in 25 samples, protein levels in 23 samples, bioenergetic profiles using an XF24 Extracellular Flux Analyzer in 6 samples, and levels of reactive oxygen species (ROS) and adenosine triphosphate (ATP) in 39 and 43 samples, respectively. In our study, the reproductive potential of AMA women sharply decreased, as expected. Additionally, an impairment in the mitochondrial function of hCCs in older women was observed; however, no differences were found in terms of mitochondrial content. Regarding oxidative phosphorylation, metabolic profiling of hCCs from AMA women indicated a decrease in respiratory capacity, which was correlated with an age-dependent decrease in the ATP synthase (ATP5A1) protein level. However, intracellular ROS and ATP levels did not differ between groups. In conclusion, our study indicates that age-related dysfunction in hCCs is associated with impaired mitochondrial function, and, although further studies are required, ATP synthase could be relevant in this impairment.

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Chemical reversion of age-related oocyte dysfunction fails to enhance embryo development in a bovine model of postovulatory aging

Ferreira,

Machado-Simões,

Moniz

et al. 2024

J Assist Reprod Genet

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Purpose There are no clinical treatments to prevent/revert age-related alterations associated with oocyte competence decline in the context of advanced maternal age. Those alterations have been attributed to oxidative stress and mitochondrial dysfunction. Our study aimed to test the hypothesis that in vitro maturation (IVM) medium supplementation with antioxidants (resveratrol or phloretin) may revert age-related oocyte competence decline. Methods Bovine immature oocytes were matured in vitro for 23 h (young) and 30 h (aged). Postovulatory aged oocytes (control group) and embryos obtained after fertilization were examined and compared with oocytes supplemented with either 2 μM of resveratrol or 6 μM phloretin (treatment groups) during IVM. Results Aged oocytes had a significantly lower mitochondrial mass and proportion of mitochondrial clustered pattern, lower ooplasmic volume, higher ROS, lower sirtuin-1 protein level, and a lower blastocyst rate in comparison to young oocytes, indicating that postovulatory oocytes have a lower quality and developmental competence, thus validating our experimental model. Supplementation of IVM medium with antioxidants prevented the generation of ROS and restored the active mitochondrial mass and pattern characteristic of younger oocytes. Moreover, sirtuin-1 protein levels were also restored but only following incubation with resveratrol. Despite these findings, the blastocyst rate of treatment groups was not significantly different from the control group, indicating that resveratrol and phloretin could not restore the oocyte competence of postovulatory aged oocytes. Conclusion Resveratrol and phloretin can both revert the age-related oxidative stress and mitochondrial dysfunction during postovulatory aging but were insufficient to enhance embryo developmental rates under our experimental conditions.

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Spatiotemporal dynamics of SIRT 1, 2 and 3 during in vitro maturation of bovine oocytes

Cited by 8 publications

References 61 publications

Resveratrol and SIRT1: Antiaging Cornerstones for Oocytes?

Resveratrol and SIRT1: Antiaging Cornerstones for Oocytes?

Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?

Chemical reversion of age-related oocyte dysfunction fails to enhance embryo development in a bovine model of postovulatory aging

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