Spatiotemporal manipulation of L-arginine release from bioactive hydrogels initiates rapid skin wound healing accompanied with repressed scar formation

Ling, Zhixin; Deng, Jing; Zhang, Zhuoran; Sui, Heyu; Shi, Wenxiong; Yuan, Bo; Lin, Hai; Yang, Xiao; Cao, Jun; Zhu, Xiangdong; Zhang, Xingdong

doi:10.1016/j.apmt.2021.101116

Cited by 21 publications

(15 citation statements)

References 44 publications

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“…As the arginine-related biosynthesis pathways were identified to be the potential underlying mechanism of TNOTN–ACD accelerating wound healing, we assessed the change of downstream factors to further validate our results. Previous studies have shown that the arginine pathway could play an important role in increasing angiogenesis and collagen production. − Therefore, we assessed the repair of the newly formed tissues by H&E, Masson, and immunofluorescence staining (Figure ). For the S.…”

Section: Resultsmentioning

confidence: 99%

On-Demand Dissociable Antibacterial Self-Healing Hydrogel with Rapid Arginine-Related Biosynthesis Capacity to Promote Full-Thickness Bacteria-Infected Wound Healing

Liu

et al. 2023

Ind. Eng. Chem. Res.

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Developing a self-healing hydrogel wound dressing with intrinsic antibacterial and on-demand dissociable properties is highly desired yet challenging in clinician applications to enhance angiogenesis and collagen growth for rapid wound healing. Herein, we present a thermoresponsive self-healing hydrogel dressing with intrinsic antimicrobial property based on a poly(Nisopropylacrylamide)-derived ABA triblock copolymer (TNOTN) and an aldehyde β-cyclodextrin (ACD) for rapid healing of fullthickness infected wounds. The prepared TNOTN−ACD hydrogel exhibits excellent thermal responsiveness with a well-defined thermal-induced sol−gel transition, allowing in situ gelling and on-demand removal of wound dressing via temperature switch. Meanwhile, the dynamic Schiff base bonds formed between TNOTN and ACD endow the hydrogel with excellent self-healing properties, which can maintain structural integrity for constant and effective antibacterial performance. Meanwhile, TNOTN−ACD hydrogels have good antibacterial properties against both S. aureus and E. coli, as well as good biocompatibility and hemocompatibility. More importantly, in vivo spatial metabolomics revealed that the TNOTN−ACD hydrogel dressing could accelerate angiogenesis and collagen deposition via the arginine-related biosynthesis pathways, thereby effectively promoting wound closure in a full-thickness bacteria-infected skin defect.

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Section: Resultsmentioning

confidence: 99%

On-Demand Dissociable Antibacterial Self-Healing Hydrogel with Rapid Arginine-Related Biosynthesis Capacity to Promote Full-Thickness Bacteria-Infected Wound Healing

Liu

et al. 2023

Ind. Eng. Chem. Res.

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“…Meanwhile, collagen III levels increased significantly after the inflammatory phase, which can reduce scar formation. Type III collagen in the connective tissue is replaced by type I collagen in the remodeling period [ 32 , 33 ]. This was closely related to the fact that the PMPPs group significantly promoted the proliferation of CD31 and FGF ( Figure 6 ), and enhanced collagen synthesis through the TGF-β/Smad signaling pathway ( Figure S2 ).…”

Section: Discussionmentioning

confidence: 99%

Investigation of the In Vivo, In Vitro, and In Silico Wound Healing Potential of Pinctada martensii Purified Peptides

et al. 2022

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Previous studies found that both oral and topical administration of enzymatic digestion products < 3 K Da ultrafiltration fractions of Pinctada martensii mantle (PMPs) had pro-healing effects. Thus, we further purified them by Sephadex-G25 and screened them by cellular assays to obtain Pinctada martensii purified peptides (PMPPs). In this study, we explored the mechanism of PMPPs on wound healing by in vivo, in vitro, and in silico experiments. LC-MS/MS results showed that PMPPs consisted of 33 peptides with molecular weights ranging from 758.43 to 2014.04 Da, and the characteristic peptide was Leu-Asp. The results of cellular assays showed that PMPPs promoted the proliferation of human skin fibroblasts (HSF) (135%) and human immortalized keratinocyte (HaCaT) cells (125%) very significantly at 12.5 μg/mL. The in vivo results showed that PMPPs could achieve scarless healing by inhibiting the inflammatory response, accelerating the epithelialization process, and regulating collagen I/III ratio. The optimal peptide sequence FAFQAEIAQLMS of PMPPs was screened for key protein receptors in wound healing (EGFR1, FGFR1, and MMP-1) with the help of molecular docking technique, which also showed to be the key pro-healing active peptide sequence. Therefore, it may provide a therapeutic strategy with great potential for wound healing.

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“…In contrast to this, we did not find any significant changes in Col3a1 gene expressions. Probably, all this is associated with the temporal dynamics of wound healing: type III collagen is gradually degraded, and type I collagen is generated at the same time 45 . O. viverini granulin has previously been shown to promote cholangiocyte proliferation in vitro 15 .…”

Section: Discussionmentioning

confidence: 99%

Wound healing approach based on excretory-secretory product and lysate of liver flukes

Ковнер

Tarasenko

Zaparina

et al. 2022

Sci Rep

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Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate proliferation of bile duct epithelium. In this study, we investigated skin wound healing potential of O. felineus proteins in mouse model. C57Bl/6 mice were inflicted with superficial wounds with 8 mm diameter. Experimental groups included several non-specific controls and specific treatment groups (excretory-secretory product and lysate). After 10 days of the experiment, the percentage of wound healing in the specific treatment groups significantly exceeded the control values. We also found that wound treatment with excretory-secretory product and worm lysate resulted in: (i) inflammation reducing, (ii) vascular response modulating, (iii) type 1 collagen deposition promoting dermal ECM remodeling. An additional proteomic analysis of excretory-secretory product and worm lysate samples was revealed 111 common proteins. The obtained data indicate a high wound-healing potential of liver fluke proteins and open prospects for further research as new therapeutic approaches.

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Spatiotemporal manipulation of L-arginine release from bioactive hydrogels initiates rapid skin wound healing accompanied with repressed scar formation

Cited by 21 publications

References 44 publications

On-Demand Dissociable Antibacterial Self-Healing Hydrogel with Rapid Arginine-Related Biosynthesis Capacity to Promote Full-Thickness Bacteria-Infected Wound Healing

On-Demand Dissociable Antibacterial Self-Healing Hydrogel with Rapid Arginine-Related Biosynthesis Capacity to Promote Full-Thickness Bacteria-Infected Wound Healing

Investigation of the In Vivo, In Vitro, and In Silico Wound Healing Potential of Pinctada martensii Purified Peptides

Wound healing approach based on excretory-secretory product and lysate of liver flukes

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