Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development

Gonzalez Lopez, Marcos; Huteckova, Barbora; Lavicky, Josef; Zezula, Nikodem; Rakultsev, Vladislav; Fridrichova, Vendula; Tuaima, Haneen; Nottmeier, Cita; Petersen, Julian; Kavkova, Michaela; Zikmund, Tomas; Kaiser, Jozef; Lav, Rupali; Star, Haza; Bryja, Vítězslav; Henyš, Petr; Vořechovský, Miroslav; Tucker, Abigail S.; Harnos, Jakub; Buchtova, Marcela; Krivanek, Jan

doi:10.1126/sciadv.adi0482

Cited by 7 publications

(1 citation statement)

References 50 publications

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“…Xenopus' teeth exhibit an acrodont type of attachment, where they form an ankylotic attachment with the adjacent bone and are characteristic by regular renewal, a condition known as polyphyodont dentition. Notably, the phenomenon of tooth renewal in Xenopus is akin to what has been previously documented in other species, such as geckos 34,35 .…”

Section: Tooth Analysismentioning

confidence: 74%

Unveiling Vertebrate Development Dynamics in FrogXenopus laevisusing Micro-CT Imaging

Jakub,

Michaela,

Alice

et al. 2024

Preprint

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BackgroundXenopus laevis, the African clawed frog, is a versatile vertebrate model organism employed across various biological disciplines, prominently in developmental biology to elucidate the intricate processes underpinning body plan reorganization during metamorphosis. Despite its widespread utility, a notable gap exists in the availability of comprehensive datasets encompassingXenopus’late developmental stages.FindingsIn the present study, we harnessed micro-computed tomography (micro-CT), a non-invasive 3D imaging technique utilizing X-rays to examine structures at a micrometer scale, to investigate the developmental dynamics and morphological changes of this crucial vertebrate model. Our approach involved generating high-resolution images and computed 3D models of developingXenopusspecimens, spanning from premetamorphosis tadpoles to fully mature adult frogs. This extensive dataset enhances our understanding of vertebrate development and is adaptable for various analyses. For instance, we conducted a thorough examination, analyzing body size, shape, and morphological features, with a specific emphasis on skeletogenesis, teeth, and organs like the brain at different stages. Our analysis yielded valuable insights into the morphological changes and structure dynamics in 3D space duringXenopus’development, some of which were not previously documented in such meticulous detail. This implies that our datasets effectively capture and thoroughly examineXenopusspecimens. Thus, these datasets hold the solid potential for additional morphological and morphometric analyses, including individual segmentation of both hard and soft tissue elements withinXenopus.ConclusionsOur repository of micro-CT scans represents a significant resource that can enhance our understanding ofXenopus’development and the associated morphological changes. The widespread utility of this amphibian species, coupled with the exceptional quality of our scans, which encompass a comprehensive series of developmental stages, opens up extensive opportunities for their broader research application. Moreover, these scans have the potential for use in virtual reality, 3D printing, and educational contexts, further expanding their value and impact.Graphical abstract & lay summary3D images of selected developmental stages ofX. laevisin a comparison (scale bar = 10 mm).Lay summaryX-ray tomography was used to examine the African clawed frog (Xenopus laevis). An extensive data set of specimens from tadpoles to adult frogs provides novel insights into the changes and developmental dynamics of selected structures, which opens avenues to an improved understanding of this crucial animal model.

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Section: Tooth Analysismentioning

confidence: 74%

Unveiling Vertebrate Development Dynamics in FrogXenopus laevisusing Micro-CT Imaging

Jakub,

Michaela,

Alice

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

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Tissue clearing and its application in dental research

Meng,

Song,

Wang

et al. 2024

BMEMat

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For both animal and human tissues, translucence is an intrinsic property that gives them a milky appearance. This optical property arises due to the combined effects of light absorption and scattering and becomes the main impediment of deep imaging. To overcome these obstacles, the tissue‐clearing technique has experienced a resurgence over the past century and evolved from its initial use in neuroscience to encompass various samples due to the emergence of various clearing methods. Notably, these techniques unveil both macroscopic and microscopic details, offering valuable insights into tissue structures. In particular, the oral cavity is structured with both soft and hard tissues at the macroscopic level and is rich in neurovascular networks microscopically, providing a suitable application environment for tissue‐clearing techniques. Currently, tissue‐clearing techniques have provided a powerful tool for research on the dental pulp neurovascular system, oral tissue regeneration, dental implants, and maxillofacial surgical treatments. Hence, this review aims to give a general introduction to tissue‐clearing techniques and focus on their remarkable applications in dental research. At last, we will discuss the integration of tissue‐clearing methods with other techniques such as labeling and microscopy, hoping to offer valuable insights for the development of tissue‐clearing techniques in both bioscience and materials science.

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Exosome-functionalized heterogeneous nanofibrous scaffolds repair bone defects accompanied by muscle injury

Jin,

Luo,

Cai

et al. 2024

Chemical Engineering Journal

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Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development

Cited by 7 publications

References 50 publications

Unveiling Vertebrate Development Dynamics in FrogXenopus laevisusing Micro-CT Imaging

Unveiling Vertebrate Development Dynamics in FrogXenopus laevisusing Micro-CT Imaging

Tissue clearing and its application in dental research

Exosome-functionalized heterogeneous nanofibrous scaffolds repair bone defects accompanied by muscle injury

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