Spatiotemporal organization and correlation of tip-focused exocytosis and endocytosis in regulating pollen tube tip growth

Weng, Xun; Shen, Yifan; Jiang, Liwen; Zhao, Lifeng; Wang, Hao

doi:10.1016/j.plantsci.2023.111633

Cited by 5 publications

(4 citation statements)

References 108 publications

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“…After 1 h germination, the pollen grain is transcriptionally ready to face PT growth by up-regulation of cytoskeleton reorganisation for anterograde/retrograde transport of vesicles and organelles, vesicular trafficking, Ca

and other ion fluxes, distribution of reactive oxygen species, pH control, energy metabolism, and protein folding and degradation [ 1 , 4 , 8 , 9 , 12 , 19 , 72 , 75 , 83 ], as was also supported results in Figure 2 and Figure 4 . This may explain why down-regulation of ‘endocytosis’, ‘secretion’ and ‘vesicle transport’ in the 0→1 h transition is followed by up-regulation of ‘organelle transport’, ‘exocytosis’, ‘endosome to vacuole transport’, ‘reorganisation of cytoskeleton’, probably due to the retrograde and anterograde movement of organelles and vesicles, including the gametes [ 7 ], that coordinate exocytosis and endocytosis for a fast pollen tube tip growth [ 2 , 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…Flowering plants generate pollen grains that germinate on the stigma and produce a pollen tube (PT)—an evolutionary innovation that is one of the fastest-growing plant-cell-derived structures—to securely deliver sperm cells into the maternal reproductive tissues toward the ovules [ 1 , 2 ]. Pollen tube growth is, therefore, essential for successful fertilisation, becoming of major importance from an agronomical viewpoint.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Insight into the Pollen Tube Growth of Olea europaea L. subsp. europaea Reveals Reprogramming and Pollen-Specific Genes Including New Transcription Factors

Bullones

Castro

Lima-Cabello

et al. 2023

Plants

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The pollen tube is a key innovation of land plants that is essential for successful fertilisation. Its development and growth have been profusely studied in model organisms, but in spite of the economic impact of olive trees, little is known regarding the genome-wide events underlying pollen hydration and growth in this species. To fill this gap, triplicate mRNA samples at 0, 1, 3, and 6 h of in vitro germination of olive cultivar Picual pollen were analysed by RNA-seq. A bioinformatics R workflow called RSeqFlow was developed contemplating the best practices described in the literature, covering from expression data filtering to differential expression and clustering, to finally propose hub genes. The resulting olive pollen transcriptome consisted of 22,418 reliable transcripts, where 5364 were differentially expressed, out of which 173 have no orthologue in plants and up to 3 of them might be pollen-specific transcription factors. Functional enrichment revealed a deep transcriptional reprogramming in mature olive pollen that is also dependent on protein stability and turnover to allow pollen tube emergence, with many hub genes related to heat shock proteins and F-box-containing proteins. Reprogramming extends to the first 3 h of growth, including processes consistent with studies performed in other plant species, such as global down-regulation of biosynthetic processes, vesicle/organelle trafficking and cytoskeleton remodelling. In the last stages, growth should be maintained from persistent transcripts. Mature pollen is equipped with transcripts to successfully cope with adverse environments, even though the in vitro growth seems to induce several stress responses. Finally, pollen-specific transcription factors were proposed as probable drivers of pollen germination in olive trees, which also shows an overall increased number of pollen-specific gene isoforms relative to other plants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptomic Insight into the Pollen Tube Growth of Olea europaea L. subsp. europaea Reveals Reprogramming and Pollen-Specific Genes Including New Transcription Factors

Bullones

Castro

Lima-Cabello

et al. 2023

Plants

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“…During tip growth, newly synthesized proteins and other material are transported and incorporated into the tips of root hairs (Moon et al 2022;Weng et al 2023;Lombardo and Lamattina 2012). ER contributes driving force for cytoplasmic streaming which assists in delivering materials to growing tip (Liu et al 2017).…”

Section: Prx102 Participates In Root Hair Tip Growthmentioning

confidence: 99%

PRX102 Participates in Root Hairs Tip Growth of Rice

Moon,

Derakhshani,

Gho

et al. 2023

Rice

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Root hairs are extensions of epidermal cells on the root tips that increase the root contract surface area with the soil. For polar tip growth, newly synthesized proteins and other materials must be incorporated into the tips of root hairs. Here, we report the characterization of PRX102, a root hair preferential endoplasmic reticulum peroxidase. During root hair growth, PRX102 has a polar localization pattern within the tip regions of root hairs but it loses this polarity after growth termination. Moreover, PRX102 participates in root hair outgrowth by regulating dense cytoplasmic streaming toward the tip. This role is distinct from those of other peroxidases playing roles in the root hairs and regulating reactive oxygen species homeostasis. RNA-seq analysis using prx102 root hairs revealed that 87 genes including glutathione S-transferase were downregulated. Our results therefore suggest a new function of peroxidase as a player in the delivery of substances to the tips of growing root hairs.

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“…In the subapical region of pollen tube, a so called "actin fringe" composed of short transverse cables 37 is thought to mediate vesicle trafficking including exocytosis and endocytosis toward the tip 38 . A recent preprint showed that the actin fringe involves the plasma membrane-anchored immunophilin-like protein TWD1, presumably through its function as adaptor for myosin-dependent cargo trafficking 39 .…”

Section: Polarized Growthmentioning

confidence: 99%