Spatiotemporal regulation and roles of reproductive phasiRNAs in plants

Komiya, Reina

doi:10.1266/ggs.21-00042

Cited by 7 publications

(5 citation statements)

References 54 publications

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“…Anther development is approximately classified into four stages: premeiosis, early meiosis, meiotic division, and microspore (Supplementary Table S1 ) 22 . Meiosis is an essential phenomenon in which genetic information is inherited by the subsequent generation 23 .…”

Section: Resultsmentioning

confidence: 99%

“…A large number of 21- and 24-nucleotide small RNAs are produced specifically in the anther and are loaded onto reproductive AGOs 20 , 28 , 29 . These anther small RNAs are spatiotemporally classified into two groups, premeiotic small RNAs in the somatic outer layers and meiotic small RNAs in the inner layers/meiocytes, during anther development 22 , 30 . Furthermore, 24-nucleotide small RNAs of the anther reportedly move from the tapetum to the meiocytes 21 , 31 , indicating the possibility of cell-to-cell interaction via small RNAs as mobile signals in anthers.…”

Section: Discussionmentioning

confidence: 99%

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3D multiple immunoimaging using whole male organs in rice

Araki

Tamotsu

Komiya

2022

Sci Rep

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Spatiotemporal regulation of proteins and RNAs is essential for the precise development of reproductive tissues in many organisms. The anther, a prominent part of the male reproductive organ in plants, contains several somatic cell layers named the anther wall and, within it, the germ cells. Here, we successfully developed a simple 3D organ-immunoimaging technique for rice anthers, which distinguishes each individual cell from the four somatic cell layers and germ cells without the need for transformation, embedding, sectioning, or clearing. The 3D immunostaining method is also applicable to the intracellular localization of meiosis-specific proteins in meiocytes, as exemplified by MEL1, a germ cell-specific ARGONAUTE in the cytoplasm, and ZEP1, a pachytene marker on meiotic chromosomes. Our 3D multiple immunostaining method with single-cell and intracellular resolution will contribute to a comprehensive organ-level elucidation of molecular mechanisms and cellular connectivity.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

3D multiple immunoimaging using whole male organs in rice

Araki

Tamotsu

Komiya

2022

Sci Rep

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“…MEL1 interacts with 21-nucleotide (nt) phasiRNAs that have cytosine at the 5′-terminal position (C-phasiRNAs), which are derived from more than 770 long non-coding RNAs (lncRNAs) named 21PHAS s during pre-meiosis and early meiosis in rice 6 . During plant reproduction, numerous 21- and 24-nt phasiRNAs are enriched in the anthers, the male organ in plants, via cleavage of miR2118/2275 and processing by Dicer-like proteins 7 – 12 . T rans -acting cleavage of 21-nt phasiRNAs in the cytoplasm of male germ cells has been detected 13 , 14 .…”

Section: Introductionmentioning

confidence: 99%

Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway

et al. 2023

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Argonaute protein (AGO) in association with small RNAs is the core machinery of RNA silencing, an essential mechanism for precise development and defense against pathogens in many organisms. Here, we identified two AGOs in rice anthers, AGO1b and AGO1d, that interact with phased small interfering RNAs (phasiRNAs) derived from numerous long non-coding RNAs. Moreover, 3D-immunoimaging and mutant analysis indicated that rice AGO1b and AGO1d cell type-specifically regulate anther development by acting as mobile carriers of these phasiRNAs from the somatic cell layers to the germ cells in anthers. Our study also highlights a new mode of reproductive RNA silencing via the specific nuclear and cytoplasmic localization of three AGOs, AGO1b, AGO1d, and MEL1, in rice pollen mother cells.

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“…1A). Anther development is approximately classified into four stages, the premeiosis, early meiosis, meiotic division, and microspore (Supplementary Table S1) 5 . The development of the somatic cell layer synchronizes with that of the germ cells, suggesting cell-to-cell communication between the soma and germ in anther development 6,7 .…”

Section: Introductionmentioning

confidence: 99%

3D-multiple immunoimaging using whole male organs in rice

Araki

Tamotsu

Komiya

2022

Preprint

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The spatiotemporal regulation of proteins and RNAs is essential for the precise development of reproductive tissues in many organisms. The anther, a significant part of the male reproductive organ in plants, contains the somatic cell layers named the anther wall and germ cells located inside. The cell-to-cell communication between the soma and germ cells in the development of male and female tissues is reported, although the mechanism remains unknown in plants. Here, we successfully developed a simple 3D-organ-immunoimaging technique, which distinguishes each single cell from the four somatic cell layers and germ cells without the need for transformation, embedding, sectioning, or clearing in rice. The 3D-immunostaining method is also applicable to the intracellular localization of meiosis-specific proteins in meiocytes-namely MEL1, a germ cell-specific Argonaute in the cytoplasm, and ZEP1, a pachytene marker on meiotic chromosomes. Our study suggests that the 3D-multiple immunostaining method with single-cell and intracellular resolution will contribute to the organ-level elucidation of comprehensive molecular mechanisms and non-cell-autonomous systems.

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Spatiotemporal regulation and roles of reproductive phasiRNAs in plants

Cited by 7 publications

References 54 publications

3D multiple immunoimaging using whole male organs in rice

3D multiple immunoimaging using whole male organs in rice

Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway

3D-multiple immunoimaging using whole male organs in rice

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