Spatiotemporal spread of grapevine red blotch-associated virus in a California vineyard

Cieniewicz, Elizabeth; Pethybridge, Sarah J.; Gorny, Adrienne M.; Madden, L. V.; McLane, Heather; Perry, Keith L.; Fuchs, Marc

doi:10.1016/j.virusres.2017.03.020

Cited by 40 publications

(26 citation statements)

References 24 publications

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“…Significant differences are indicated by *, **, and *** for significance at P # 0.05, 0.01, and 0.001, respectively. through insect vectors (Cieniewicz et al 2017;Sudarshana et al 2015). The cornered alfalfa treehopper Spissistilus festinus was recently shown to transmit the virus under experimental conditions (Bahder et al 2016a).…”

Section: Discussionmentioning

confidence: 99%

Grapevine red blotch virus: Absence in Swiss Vineyards and Analysis of Potential Detrimental Effect on Viticultural Performance

et al. 2018

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Grapevine red blotch virus (GRBV) is a recently described virus that infects grapevine. Little information is available on the possible occurrence and distribution outside North America. Therefore, we surveyed commercial vineyards from the three major grape-growing regions in Switzerland to determine the presence or absence of GRBV. In total, 3,062 vines were analyzed by polymerase chain reaction. None of the vines tested positive for GRBV, suggesting the absence of GRBV from Swiss vineyards. We also investigated whether GRBV was present in 653 grapevine accessions in the Agroscope grapevine virus collection at Nyon, including dominantly Swiss (457) but also international accessions. Only six referential accessions were infected by GRBV, all originating from the United States, whereas all others from 10 European and 8 non-European origins tested negative. High-throughput sequencing analysis of Zinfandel A2V13, in the collection since 1985, confirmed close similarity of GRBV isolate Z_A2V13 to American isolates according to genomes deposited in GenBank. Because the Zinfandel A2V13 reference was also maintained grafted on the leafroll virus indicator Vitis vinifera ‘Gamay’, we evaluated the effect of GRBV on viticultural performance over a 3-year period. Our results showed clear detrimental effects of GRBV on grapevine physiology (vine vigor, leaf chlorophyll content, and gas exchange) and fruit quality. These findings underscore the importance of implementation of GRBV testing worldwide in certification and quarantine programs to prevent the dissemination of this virus.

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Section: Discussionmentioning

confidence: 99%

Grapevine red blotch virus: Absence in Swiss Vineyards and Analysis of Potential Detrimental Effect on Viticultural Performance

et al. 2018

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“…Since its discovery in 2008 in California (Calvi, 2011), Grapevine Red Blotch Disease (GRBD)-and its recently confirmed causal agent Grapevine Red Blotch Virus (GRBV) (Yepes et al, 2018)has significantly impacted several major grape-growing areas in the United States (Sudarshana and Zalom, 2017). To date, many research groups have been actively investigating various aspects of GRBD, but most have been focused either on identification and detection (Krenz et al, 2012;Al Rwahnih et al, 2013;Buchs et al, 2018;Romero et al, 2019), genetics and virology (Al Rwahnih et al, 2015;Sudarshana et al, 2015), vector biology and transmission (Bahder et al, 2016;Preto et al, 2018a;Preto et al, 2018b), or disease epidemiology and spread (Cieniewicz et al, 2017;Cieniewicz et al, 2018;Dalton et al, 2019). However, no agronomic studies have been conducted testing interactions between cultural practices and their effects on physiology of GRBV-infected grapevines.…”

Section: Introductionmentioning

confidence: 99%

Water Deficits Do Not Improve Fruit Quality in Grapevine Red Blotch Virus-Infected Grapevines (Vitis vinifera L.)

Levin

2020

Front. Plant Sci.

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Although deficit irrigation is used to improve fruit quality in healthy grapevines, it can potentially amplify negative effects of viral disease and reduce fruit quality in Grapevine Red Blotch Virus (GRBV) infected grapevines. Therefore, a 2-year field experiment was conducted to understand the interaction between GRBV infection and water deficits on disease development and vine physiology. Well-watered (WW) vines were irrigated at 100% of estimated crop evapotranspiration (ET c), while water deficit (WD) vines received water at 66 and 50% ET c in 2017 and 2018, respectively. Healthy (GRBV-) and infected (GRBV+) vines were confirmed by PCR assays. There were no significant effects of water deficits on foliar symptom onset in either year, but more severe water deficits in 2018 resulted in a more rapid symptom progression. GRBV+ vines had a higher Y stem compared to GRBV-vines, but the effects of virus only appeared post-veraison and corresponded to decreased leaf gas exchange. In general, vine vegetative and reproductive growth were not reduced in GRBV+ vines. Yields were highest in WW/ GRBV+ vines due to larger clusters containing larger berries. Consistent treatment effects on berry primary chemistry were limited to sugars, with no interactions between factors. Water deficits were able to somewhat increase berry anthocyanin concentration in GRBV + fruit, but the effects were dependent on year. By comparison, virus status and water deficits interacted on skin tannins concentration such that they were decreased in WD/ GRBV+ vines, but increased in WD/GRBV-vines. Water deficits had no effect on seed phenolics, with only virus status having a significant diminution. Although keeping GRBV+ vines well-watered may mitigate some of the negative effects of GRBD, these results suggest that water deficits will not improve overall fruit quality in GRBV+ vines. Ultimately, the control of fruit ripening imparted by GRBV infection seems to be stronger than abiotic control imparted by water deficits.

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“…En este estudio se determinaron asociaciones espaciales significativas de los índices de agrupamiento entre las temporadas sucesivas, lo que se sugiere que el grado de agregación espacial de las vides enfermas fue asociado con la posición espacial de las mismas en el año anterior. Lo cual indica que GRBV puede extenderse con el tiempo, dentro y entre filas del viñedo donde se presenten vides infectadas (Cieniewicz et al 2017b).…”

Section: Distribución De Grbvunclassified

“…El diagnóstico visual se basa en la sintomatología expresada en la planta y este es el primero y más utilizado método para diagnosticar enfermedades en la vid; sin embargo, se basa en la experiencia por lo que puede arrojar una identificación incorrecta del patógeno (Zherdev et al, 2018). Existen múltiples reportes que la severidad de los síntomas del GRBV varían según el cultivar y la estación del año (Sudarshana et al, 2015;Cieniewicz et al, 2017b). Además, un diagnóstico visual puede ser complicado debido a las similitudes de los síntomas foliares y en frutos entre GRBV, el virus del enrollamiento de la hoja (GLRaV) y la enfermedad de Pierce (Cieniewicz et al, 2017a;Zherdev et al, 2018).…”

Section: Diagnóstico Visualunclassified

Virus De La “Mancha Roja” De La Vid: Una Potencial Enfermedad en Los Viñedos De México

Beltrán-Beache¹,

Fuentes²,

Cerna-Chávez³

et al. 2021

Trop Subtrop Agroecosyst

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Background. The grapevine red blotch virus (GRBV) is a member of the Geminiviridae family that has a single-stranded DNA genome consisting of 3,206 nucleotides, from which two clades (1 and 2) diverge with differences of up to 8.5% in the genome. The symptoms associated with the red blotch disease are leaves with irregular red spots, the veins turn red and occasionally margins of the same color can be observed; white grape cultivars symptoms are less marked, involving slight chlorosis of irregular shape that may become necrotic as time, causing delays in fruit maturation and alterations in the chemical characteristics of the juice. Objective. Introduce the current situation of the grapevine red blotch virus on the vine worldwide and the risk that it represents as an emerging disease in Mexico, as well as the available detection methods and strategies for managing the disease and its known vectors. Methodology. Employed method was a literature review about Grapevine “red spot” virus and its damages on the vine plants. Main findings. A review is obtained with the distribution of GRBV worldwide, highlighting the main differences with Pierce disease and GLRaV, knowledge of the vectors and the amin detection techniques. Implications. The real spread of the red blotch disease caused by GRBV in Mexico, specific vectors or alternative hosts is not known, which represents a latent risk to the national grape production. Communicate timely and available information about possible vectors, detection techniques and symptoms caused by GRBV are very important for a monitoring and control strategy design. Conclusions. The disease caused by GRBV has been recently detected in Mexico country, making crucial to establish effective and reliable detection strategies that improve the monitoring and control of GRBV and its possible vectors. This review shows the current panorama of the GRBV distribution in Mexico and the world, the repercussions on the crop due to the effect of the symptoms that affect the physiology and metabolism of the plants, which affects the production and quality fruit, as well as the information available to support the vine production systems in Mexico.

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Spatiotemporal spread of grapevine red blotch-associated virus in a California vineyard

Cited by 40 publications

References 24 publications

Grapevine red blotch virus: Absence in Swiss Vineyards and Analysis of Potential Detrimental Effect on Viticultural Performance

Grapevine red blotch virus: Absence in Swiss Vineyards and Analysis of Potential Detrimental Effect on Viticultural Performance

Water Deficits Do Not Improve Fruit Quality in Grapevine Red Blotch Virus-Infected Grapevines (Vitis vinifera L.)

Virus De La “Mancha Roja” De La Vid: Una Potencial Enfermedad en Los Viñedos De México

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