Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of a crude gonadotropin-releasing hormone analogue

Amezawa, Kotaro; Yazawa, Ryosuke; Takeuchi, Yutaka; Yoshizaki, Goro

doi:10.1007/s12562-018-1185-6

Cited by 16 publications

(7 citation statements)

References 28 publications

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“…In addition, seed production by natural spawning would have the advantage of requiring less labour than artificial insemination. We have previously reported that orally delivered GnRHa can induce spawning in chub mackerel, which would be an alternative to the implantation of GnRHa pellets (Amezawa et al, 2018). Using this non‐invasive spawning induction method, it would be possible to produce the donor‐derived seedlings without any physical contact of the parental fish.…”

Section: Discussionmentioning

confidence: 99%

“…To confirm the production of donor‐derived eggs by female recipients, PCR‐RFLP analysis of mtDNA was performed in the same manner as described for the male recipients. Ovulation was induced by implantation of sustained‐release cholesterol pellets containing GnRHa at a dose of 100 μg/kg BW into six female recipients, as described by Amezawa et al (2018). Eggs were collected from ovulated female recipients by applying gentle pressure to the abdomen at 36 h after pellet implantation.…”

Section: Methodsmentioning

confidence: 99%

“…To confirm whether the eggs obtained from the female recipients were functional and whether it was possible to produce donor‐derived F1 offspring by natural spawning of male and female hybrid recipients, group mating experiments were conducted with sexually mature hybrid recipients (two females [the same recipient fish as described in Section 2.5] and 35 males) in a 5000‐L FRP tank. Spawning was induced by the implantation of sustained‐release cholesterol pellets containing GnRHa at a dose of 100 μg/kg BW, as described by Amezawa et al (2018). Floating eggs were collected using an egg collection net attached to the drain of the fish tank.…”

Section: Methodsmentioning

confidence: 99%

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Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation

Tani

Yazawa

Kamio

et al. 2022

Aquaculture Research

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In this study, we examined the feasibility of germ cell‐less hybrids, produced by a cross between female blue mackerel (Scomber australasicus) and male chub mackerel (S. japonicus), as recipients in order to develop a surrogate broodstock technology for mackerels, which is attracting attention as a new aquaculture target. Donor testicular cells, isolated from the testes of a chub mackerel, were transplanted into the peritoneal cavity of 500 hybrid mackerel larvae. Of the 42 male recipients that survived to 1 year of age, 35 matured and produced donor‐derived chub mackerel sperm. Amongst them, 22 produced only donor‐derived sperm, with none of the recipient's own sperm detected. Two of the six females that survived to 1‐year‐old produced donor‐derived eggs, and one of two matured females produced only donor‐derived eggs. Notably, when the mature recipients were housed in a single fish tank, some spontaneously mated, and normal hatchlings arising from fertilization of donor‐derived chub mackerel eggs and sperm were successfully obtained. Although the efficiency of this method needs to be improved in the future, hybrid mackerel can be used as surrogates for mackerel species breeding. This technology is expected to make a significant contribution to the breeding of mackerels.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation

Tani

Yazawa

Kamio

et al. 2022

Aquaculture Research

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“…Since ELT broodstock continued to spawn daily for 1–2 weeks by a single administration of GnRHa which can be performed with a gentle treatment, it was possible to repeatedly produce triploid populations without loss of valuable broodstock (Yazawa et al, ). Additionally, we recently reported that orally delivered GnRHa could induce the spawning of ELT and blue mackerel ( Scomber australasicus ) as an alternative to the implantation of a GnRHa pellet (Amezawa, Takeuchi, Yazawa, & Yoshizaki, ). Using both this ‘non‐invasive’ administration method of GnRHa for the spawning induction and the triploidization technique using naturally spawned eggs established in this study, it may be possible to induce triploidy without any physical contact with the broodstock.…”

Section: Discussionmentioning

confidence: 99%

Production of triploid eastern little tuna,Euthynnus affinis(Cantor, 1849)

et al. 2019

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Herein, we developed a triploidization method using spawned eggs collected immediately after spawning of eastern little tuna (ELT), Euthynnus affinis. ELT broodstock induced the spawning by hormonal treatment in the tank, with the resulting spawned eggs being used for the triploidy induction. Under optimal conditions, the mean ± SEM triploidization and hatching rates were 97.2 ± 2.8% and 84.5 ± 10.3%, respectively. Although triploid ELT showed growth performance equivalent to that of diploids, the triploids died at a higher rate than the diploids during 2–4 weeks post‐hatching when triploids and diploids were reared in the same tank. Therefore, we propose that it would be necessary in a practical operation to use triploid‐only ELT seedlings to avoid selective cannibalism by the diploids. The ELT triploids exhibited an all‐female phenotype. Because previous studies have reported that female triploids show a greater probability of sterility than male triploids, this characteristic could be a major advantage. Since this triploidization method, using spawned eggs, can be performed without handling the broodstock, it is possible to avoid the physical damage caused by the process of artificial insemination, making it possible to repeatedly produce triploid populations without valuable broodstock loss. Thus, we have developed an efficient method to produce ELT triploids, although further study is essential to evaluate sterility of the triploid ELT.

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“…To maintain sustained release of GnRHa, which in turn provides long-term elevation of circulating Lh, solid pellets are often prepared by mixing GnRHa with cholesterol or a co-polymer, such as ethylene and vinyl acetate (Zohar & Mylonas, 2001). Implantating of GnRHa-containing pellets has been widely used to induce ovulation and spawning (Amezawa et al, 2018;Mylonas & Zohar, 2007;Yazawa et al, 2015). In contrast, Fsh is considered essential for early oogenesis and vitellogenesis in teleosts, especially salmonids (Luckenbach et al, 2013), but there is relatively little information of Fsh release in response to GnRH.…”

mentioning

confidence: 99%

Effect of GnRHa on plasma levels of Fsh and Lh in the female greater amberjack Seriola dumerili

Nyuji

Yamamoto

Hamada³

et al. 2019

Journal of Fish Biology

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The effects of gonadotropin‐releasing hormone agonist (GnRHa) on plasma levels of follicle‐stimulating hormone (Fsh) and luteinising hormone (Lh) are reported for female greater amberjack Seriola dumerili with post‐vitellogenic ovarian oocytes. Five females were implanted with pellets containing GnRHa (600 μg kg−1 body weight), while five other females were injected with saline. All females implanted with GnRHa‐containing pellets ovulated 36–42 h post‐implantation. The GnRHa implants elevated Lh, but not Fsh plasma levels within 42 h of GnRHa administration.

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Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of a crude gonadotropin-releasing hormone analogue

Cited by 16 publications

References 28 publications

Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation

Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation

Production of triploid eastern little tuna,Euthynnus affinis(Cantor, 1849)

Effect of GnRHa on plasma levels of Fsh and Lh in the female greater amberjack Seriola dumerili

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