Speaking the language of lipids: the cross-talk between plants and pathogens in defence and disease

Cavaco, Ana Rita; Matos, Ana Rita; Figueiredo, Andreia

doi:10.1007/s00018-021-03791-0

Cited by 36 publications

(14 citation statements)

References 173 publications

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“…Lipids were shown to be modulated upon inoculation with the tolerant grapevine Vitis vinifera cv. Regent with P. viticola, particularly at 6 and 12hpi, which decreased when compared to mockinoculated samples (Cavaco et al, 2021). Altogether, the present findings confirm the damages caused by the A. alternata fungus on C. Maritima growth and physiology, and the possibility of the involvement of salt in the mitigation of the effect of fungal attack will be discussed in the next section.…”

Section: Discussion Discussion Discussionsupporting

confidence: 83%

Comparative study of the effect of salt stress, Alternaria alternata attack or combined stress on the Cakile maritima growth and physiological performance

Chalbi

Sghaier-Hammami

Baazaoui

et al. 2021

Not Bot Horti Agrobo

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Cakile maritima is a halophytic plant model that is well known by its ability to tolerate high salt concentrations. Salinity was reported to improve the tolerance of halophytes to several abiotic stresses; however, the involvement of salt in the tolerance to biotic stress is still scant. In the present work, the effect of salt on C. maritima responses towards the pathogenic Alternaria alternata was investigated. For that, C. maritima seeds were germinated for four weeks. Plants were then divided into four groups: i) Plants irrigated with salt (200mM NaCl); ii) Plants infested by fungus; iii) Plants irrigated with salt and infested by fungus and finally control plants (0mM NaCl, without inoculation). Our results showed that upon salt stress or fungal attack, plants reduced biomass production, hydration status and photosynthetic performance which were associated with a decrease in the gas exchange and chlorophyll fluorescence parameters, with a more pronounced effect upon fungal attack. However, under combined stress, a significant increase of these parameters was noticed, with a level close to that of control. Concerning nutrient contents, K, Zn, Fe, Cu and Mg decreased in the C. maritima leaves exposed to both stresses applied individually. In contrast, all these nutrients were increased in plants grown under combined stress. Taken together, we can conclude that plants grown under combined stresses had better growth rate and physiological performance compared to all other treated plants, and that salt may be the key in improving the C. maritima ability to tolerate fungal attack.

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Section: Discussion Discussion Discussionsupporting

confidence: 83%

Comparative study of the effect of salt stress, Alternaria alternata attack or combined stress on the Cakile maritima growth and physiological performance

Chalbi

Sghaier-Hammami

Baazaoui

et al. 2021

Not Bot Horti Agrobo

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“…Lysophospholipids are usually low abundant, but can rapidly increase as signaling molecules during wounding ( Okazaki and Saito, 2014 ). Lipids are also important for plant immunity, such as the use of polyunsaturated FAs from galactolipids of the plastids to synthesize jasmonic acid, an important signaling molecule ( Cavaco et al., 2021 ). Triacylglycerols (TGs) are a major energy reserve that accumulate during seed development to be used when seeds germinate, but also have important economic and commercial value ( Durrett et al., 2008 ; Dyer et al., 2008 ).…”

Section: Introductionmentioning

confidence: 99%

A lipidomics platform to analyze the fatty acid compositions of non-polar and polar lipid molecular species from plant tissues: Examples from developing seeds and seedlings of pennycress (Thlaspi arvense)

Romsdahl

Cocuron

Pearson³

et al. 2022

Front. Plant Sci.

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The lipidome comprises the total content of molecular species of each lipid class, and is measured using the analytical techniques of lipidomics. Many liquid chromatography-mass spectrometry (LC-MS) methods have previously been described to characterize the lipidome. However, many lipidomic approaches may not fully uncover the subtleties of lipid molecular species, such as the full fatty acid (FA) composition of certain lipid classes. Here, we describe a stepwise targeted lipidomics approach to characterize the polar and non-polar lipid classes using complementary LC-MS methods. Our “polar” method measures 260 molecular species across 12 polar lipid classes, and is performed using hydrophilic interaction chromatography (HILIC) on a NH2 column to separate lipid classes by their headgroup. Our “non-polar” method measures 254 molecular species across three non-polar lipid classes, separating molecular species on their FA characteristics by reverse phase (RP) chromatography on a C30 column. Five different extraction methods were compared, with an MTBE-based extraction chosen for the final lipidomics workflow. A state-of-the-art strategy to determine and relatively quantify the FA composition of triacylglycerols is also described. This lipidomics workflow was applied to developing, mature, and germinated pennycress seeds/seedlings and found unexpected changes among several lipid molecular species. During development, diacylglycerols predominantly contained long chain length FAs, which contrasted with the very long chain FAs of triacylglycerols in mature seeds. Potential metabolic explanations are discussed. The lack of very long chain fatty acids in diacylglycerols of germinating seeds may indicate very long chain FAs, such as erucic acid, are preferentially channeled into beta-oxidation for energy production.

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“…Notably, plant-derived EVs were shown to contain stress response-related proteins and lipids [8,12,32,45,47] and exhibit antifungal activity [12,48]. It is therefore surprising that we did not observe any inhibitory effects of barley EVs on F. graminearum.…”

Section: Discussionmentioning

confidence: 83%

“…Although plant EVs were first described in the apoplast in 1967 [18], it was almost half a century before they were separated from plant apoplastic fluids and then visualized with transmission electron microscopy (TEM) [42,43,45]. These pioneering works have laid the foundation for examining the role or contribution of EVs during plantpathogen interactions [6,8,12,32,59]. These examples intensify the assumption that plant-or pathogen-derived EVs contribute bidirectionally to this highly specialized interspecies communication through the release of lipids, proteins, and small RNAs (sRNAs) that regulate and deregulate defensive and offensive responses [5,51].…”

Section: Open Accessmentioning

confidence: 99%

Extracellular vesicles isolated from dsRNA-sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

Schlemmer

Lischka

Wegner

et al. 2022

Fungal Biol Biotechnol

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Numerous reports have shown that incorporating a double-stranded RNA (dsRNA)-expressing transgene into plants or applying dsRNA by spraying it onto their leaves successfully protects them against invading pathogens exploiting the mechanism of RNA interference (RNAi). How dsRNAs or siRNAs are transferred between donor host cells and recipient fungal cells is largely unknown. It is speculated that plant extracellular vesicles (EVs) function as RNA shuttles between plants and their pathogens. Recently, we found that EVs isolated from host-induced gene silencing (HIGS) or spray-induced gene silencing (SIGS) plants contained dsRNA-derived siRNAs. In this study, we evaluated whether isolated EVs from dsRNA-sprayed barley (Hordeum vulgare) plants affected the growth of the phytopathogenic ascomycete Fusarium graminearum. Encouraged by our previous finding that dropping barley-derived EVs on F. graminearum cultures caused fungal stress phenotypes, we conducted an in vitro growth experiment in microtiter plates where we co-cultivated F. graminearum with plant EVs isolated from dsRNA-sprayed barley leaves. We observed that co-cultivation of F. graminearum macroconidia with barley EVs did not affect fungal growth. Furthermore, plant EVs containing SIGS-derived siRNA appeared not to affect F. graminearum growth and showed no gene silencing activity on F. graminearum CYP51 genes. Based on our findings, we concluded that either the amount of SIGS-derived siRNA was insufficient to induce target gene silencing in F. graminearum, indicating that the role of EVs in SIGS is minor, or that F. graminearum uptake of plant EVs from liquid cultures was inefficient or impossible.

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Speaking the language of lipids: the cross-talk between plants and pathogens in defence and disease

Cited by 36 publications

References 173 publications

Comparative study of the effect of salt stress, Alternaria alternata attack or combined stress on the Cakile maritima growth and physiological performance

Comparative study of the effect of salt stress, Alternaria alternata attack or combined stress on the Cakile maritima growth and physiological performance

A lipidomics platform to analyze the fatty acid compositions of non-polar and polar lipid molecular species from plant tissues: Examples from developing seeds and seedlings of pennycress (Thlaspi arvense)

Extracellular vesicles isolated from dsRNA-sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

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