Speaking with the Dead in Early America

Seeman, Erik R.

doi:10.9783/9780812296419

Cited by 3 publications

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“…baumannii ( n =100 assemblies, described above, mapped against the A. baumannii AYE NC_010410.1 reference) were created using Snippy (4.6.0) and Gubbins (v.3.1.3), where Gubbins highlighted areas of recombination [50, 51]. Recombination predictions were visualized in Phandango [52].…”

Section: Methodsmentioning

confidence: 99%

RND pumps across the genus Acinetobacter: AdeIJK is the universal efflux pump

et al. 2023

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Acinetobacter are generally soil-dwelling organisms that can also cause serious human infections. A. baumannii is one of the most common causative agents of Acinetobacter infections and is often multidrug resistant. However, an additional 25 species within the genus have also been associated with infection. A. baumannii encodes six resistance nodulation division (RND) efflux pumps, the most clinically relevant class of efflux pumps for antibiotic export, but the distribution and types of RND efflux pumps across the genus is currently unknown. Sixty-four species making up the genus Acinetobacter were searched for RND systems within their genomes. We also developed a novel method using conserved RND residues to predict the total number of RND proteins including currently undescribed RND pump proteins. The total number of RND proteins differed both within a species and across the genus. Species associated with infection tended to encode more pumps. AdeIJK/AdeXYZ was found in all searched species of Acinetobacter, and through genomic, structural and phenotypic work we show that these genes are actually homologues of the same system. This interpretation is further supported by structural analysis of the potential drug-binding determinants of the associated RND-transporters, which reveal their close similarity to each other, and distinctiveness from other RND-pumps in Acinetobacter , such as AdeB. Therefore, we conclude that AdeIJK is the fundamental RND system for species in the genus Acinetobacter . AdeIJK can export a broad range of antibiotics and provides crucial functions within the cell, for example lipid modulation of the cell membrane, and therefore it is likely that all Acinetobacter require AdeIJK for survival and homeostasis. In contrast, additional RND systems, such as AdeABC and AdeFGH, were only found in a subset of Acinetobacter that are associated with infection. By understanding the roles and mechanisms of RND efflux systems in Acinetobacter , treatments for infections can avoid efflux-mediated resistance and improve patient outcomes.

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Section: Methodsmentioning

confidence: 99%

RND pumps across the genus Acinetobacter: AdeIJK is the universal efflux pump

et al. 2023

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“…Long-read assemblies produced using Flye v2.9 ( 15 ) were used for hybrid assembly using Unicycler v0.5.0 ( n = 156) ( 16 ) or SPAdes v3.15.4 ( n = 49) ( 17 ). The hybrid assemblies were polished with the short reads using Pilon v1.24 ( 18 ) (with MUMmer v4.0.0 [ 19 ] and SAMtools v1.13 [ 20 ]) and Snippy v4.6.0 ( 21 ) (with VCFtools v0.1.16 [ 22 ] and Tabix v0.2.6 [ 20 ]). The assembly quality was assessed using QUAST v5.0.2 ( 23 ).…”

Section: Announcementmentioning

confidence: 99%

Hybrid Genome Assemblies of 245 Avian and Broiler Barn Environment-Associated Escherichia coli Strains Isolated from Saskatchewan Broiler Farms

Sanderson

Nnajide

McCarthy

et al. 2023

Microbiol Resour Announc

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Escherichia coli infections in poultry cause significant morbidity and economic losses for producers each year. In a 3-year period, we collected and sequenced the whole genomes of E. coli disease isolates ( n = 91), isolates from presumed healthy birds ( n = 61), and isolates from 8 barn sites ( n = 93) on broiler farms in Saskatchewan.

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“…Justin T. Clark's City of Second Sight (2018, p. 6) investigated how nineteenth‐century Boston became “the unrivaled capital of visionary culture.” Following Promey and Morgan, Clark maintained that the development of Boston and its white, middle class hinged on religious ways of seeing. Erik R. Seeman's Speaking with the Dead in Early America (2019) traced the ways white American Protestants maintained material relationships with the dead from colonial times to the mid‐1800s. Applying the work of McDannell, Morgan, Promey, and Orsi to Protestant practices with the dead, Seeman argued that Protestants spoke to the dead literally and imaginatively as they engaged elegies, gravestones, literature, objects, and ghosts.…”

Section: Influences Challenges and Areas For Further Studymentioning

confidence: 99%

The material study of American Protestantism

Brummitt

2022

Religion Compass

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This article traces the history of the material study of American Protestantism from the 1980s to today. In the 1980s, a few scholars of American religions started to incorporate material culture studies into their work. They examined religious images, objects, places, and practices according to new interdisciplinary methods. By the mid‐1990s, the material study of American Protestantism exploded and emerged as a distinct subfield in the study of American religions. This subfield gave way to four general approaches, including studying American Protestantism as print culture, visual culture, material sensations, and new materialism. This article also presents themes that invigorate the subfield today, such as studying presence in Protestantism, texts as objects, the agency of objects, Black religious experiences, gender and sexuality, and cross‐cultural encounters. It concludes by offering insights into the influences and challenges of the subfield, as well as areas in the subfield that invite more research.

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Speaking with the Dead in Early America

Cited by 3 publications

References 0 publications

RND pumps across the genus Acinetobacter: AdeIJK is the universal efflux pump

RND pumps across the genus Acinetobacter: AdeIJK is the universal efflux pump

Hybrid Genome Assemblies of 245 Avian and Broiler Barn Environment-Associated Escherichia coli Strains Isolated from Saskatchewan Broiler Farms

The material study of American Protestantism

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