Special Issue: Bacteriophage Treatment as an Alternative Technology to Inactivate Pathogenic Bacteria: A Generalized Worldwide Growing Acceptance

Almeida, Adelaide

doi:10.3390/microorganisms10010012

Cited by 1 publication

(1 citation statement)

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“…Such studies may lead to the future application of phage VP4 as a candidate for water decontamination or in phage cocktails. Applying phages in nonclinical fields, such as environments with great variability in parameters throughout the year, necessitates the study of the phage application impact on the environment and the effect of abiotic factors like temperature, pH, and UV radiation on the viability of phages [33].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic characterization of phage vB_vcM_Kuja

et al. 2023

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Bacteriophage therapy targeting the increasingly resistant Vibrio cholerae is highly needed. Hence, studying the phenotypic behavior of potential phages under different conditions is a prerequisite to delivering the phage in an active infective form. The objective of this study was to characterize phage VP4 (vB_vcM_Kuja), an environmental vibriophage isolated from River Kuja in Migori County, Kenya in 2015. The phenotypic characteristics of the phage were determined using a one-step growth curve, restriction digestion profile, pH, and temperature stability tests. The results revealed that the phage is stable through a wide range of temperatures (20−50°C) and maintains its plaque-forming ability at pH ranging from 6 to 12. The one-step growth curve showed a latent period falling between 40 and 60 min, while burst size ranged from 23 to 30 plaque-forming units/10 µl at the same host strain. The restriction digestion pattern using EcoRI, SalI, HindIII, and XhoI enzymes showed that HindIII could cut the phage genome. The phage DNA could not be restricted by the other three enzymes. The findings of this study can be used in future studies to determine phage−host interactions.

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Section: Discussionmentioning

confidence: 99%