Special Issue: Microorganisms and Plant Nutrition

Beneficial microorganisms can protect crop from phytopathogens, and modify rhizosphere microbiome. However, it is not well understood whether or how do rhizosphere microorganisms which respond to bio-agents contribute to disease suppression. Bacillus velezensis BER1 and tomato bacterial wilt caused by Ralstonia solanacearum were selected as models to disentangle the interactions and mechanisms in the rhizosphere. B. velezensis BER1 greatly suppressed tomato bacterial wilt by over 49.0%, reduced R. solanacearum colonization in the rhizosphere by 36.3%, and significantly enriched two Flavobacterium ASVs (1357 and 2401). A novel colony Loop-Mediated Isothermal Amplification (LAMP) assay system was developed to screen out Flavobacterium from tomato rhizosphere bacterial isolates. In vitro tests revealed that co-cultivating BER1 with Flavobacterium C45 increased biofilm formation by 18.6%. Climate chamber experiment further revealed that Flavobacterium C45 improved the control efficiency of BER1 on tomato bacterial wilt by 46.0%, decreased the colonization of R. solanacearum in the rhizosphere by 43.1% and elevated the transcription of plant defense gene PR1α in tomato by 45.4%. In summary, Flavobacterium C45 boosted the ability of B. velezensis BER1 to prevent bacterial wilt and the colonization of R. solanacearum, highlighting the importance of helper bacteria on elevating the efficiency of biological control.

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Bacillus velezensis BER1 enriched Flavobacterium daejeonense-like bacterium in the rhizosphere of tomato against bacterial wilt

Wang

Ding

Chen

et al. 2023

FEMS Microbiology Ecology

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Two species-specific TaqMan-based quantitative polymerase chain reaction assays for the detection in soil ofPaenibacillus polymyxainocula

Manfredini¹,

Malusà

Trzcinski

et al. 2022

Journal of Applied Microbiology

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Aims The increasingly widespread use of beneficial microbial inocula in agriculture gives rise to two primary needs: i) the assessment of the environmental risk, i.e. their impact on local soil microbiome and soil properties; ii) being able to track them and monitor their persistence and fate to both optimize their formulation and application method. In previous years, PCR-based methods have detected bacterial or fungal bioinoculant at the species or strain level. However, the selective detection, quantification, and monitoring of target microbial species in a complex ecosystem such as soil require that the tests possess high specificity and sensitivity. Methods and results The work proposes a quantitative real-time PCR detection method using TaqMan chemistry, showing high specificity and sensitivity for the Paenibacillus polymyxa K16 strain. The primer and probe sets were designed using the polymyxin gene cluster targeting pmxC and pmxE sequences. Validation tests showed that these assays allowed a discriminant and specific detection of P. polymyxa K16 in soil. Conclusion The TaqMan-assay developed could thus ensure the necessary level of discrimination required by commercial and regulatory purposes to detect and monitor the bioinoculant in soil.

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Synergy Between Microbial Inoculants and Mineral Fertilization to Enhance the Yield and Nutritional Quality of Maize on the Peruvian Coast

López-Montañez,

Calero-Rios,

Quispe

et al. 2024

Applied Microbiology

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Hard yellow maize is a crucial crop in Peruvian agriculture that plays a significant role in food security and livestock production. However, intensive fertilization practices in agronomic management have negatively impacted soil health. To explore more sustainable agricultural technologies, researchers investigated solutions using microorganisms to enhance plant growth. This study assessed the synergistic effects of microbial inoculants and mineral fertilization on INIA 619 and Dekal B-7088 maize varieties’ yield and nutritional quality. A split-plot design was employed, incorporating four inoculation treatments—no inoculant, Bacillus subtilis, Trichoderma viride, and Pseudomonas putida—combined with fertilization levels of 0%, 50%, 75%, and 100%. The findings revealed that Bacillus subtilis boosted yields by 13.1% in INIA 619 and 55.5% in Dekal B-7088. Additionally, combined with 100% fertilization, microbial inoculation increased protein content by 47% and carbohydrates by 6% in INIA 619 while maintaining nutritional quality with 75% fertilization. Similarly, in Dekal B-7088, inoculation with total fertilization enhanced protein content by 54% and fiber by 27%. These results demonstrated that microbial inoculation could reduce mineral fertilization by up to 25% while sustaining high yields and improving the nutritional quality of maize.

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Special Issue: Microorganisms and Plant Nutrition

Abstract: Plant-beneficial microorganisms affect plant nutrition and health, as a key part of prebiotic-, probiotic-, and symbiotic-based interactions [...]

Cited by 3 publications

References 11 publications

Bacillus velezensis BER1 enriched Flavobacterium daejeonense-like bacterium in the rhizosphere of tomato against bacterial wilt

Bacillus velezensis BER1 enriched Flavobacterium daejeonense-like bacterium in the rhizosphere of tomato against bacterial wilt

Two species-specific TaqMan-based quantitative polymerase chain reaction assays for the detection in soil ofPaenibacillus polymyxainocula

Synergy Between Microbial Inoculants and Mineral Fertilization to Enhance the Yield and Nutritional Quality of Maize on the Peruvian Coast

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