Special Issue: Natural Substances against Insect Pests: Assets and Liabilities

Conti, Bárbara

doi:10.3390/insects12030244

Cited by 1 publication

(1 citation statement)

References 15 publications

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“…Finding new plant-derived products against mosquitoes could help us to improve our arsenal against mosquitoes, and furthermore, to reverse the negative impacts on human health [ 9 , 10 ]. Several plant ingredients have been considered for evaluation as potential deterrents, repellents and insecticidal agents against several insect pest species [ 11 , 12 ]. Essential oils derived from plants and their major component terpenes, can be environmentally-friendly, have a low-risk profile for mammals and humans and might provide natural alternatives instead of using conventional insecticides [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Volatile Compounds with Repellent Properties against Aedes albopictus (Diptera: Culicidae) Using CPC Technology

et al. 2021

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The present work describes the use of Centrifugal Partition Chromatography (CPC) for the bio-guided isolation of repellent active volatile compounds from essential oils. Five essential oils (EOs) obtained from three Pinus and two Juniperus species were initially analyzed by gas chromatography–mass spectrometry (GC/MS) and evaluated for their repellent properties against Aedes albopictus. The essential oil from needles of P. pinea (PPI) presented the higher activity, showing 82.4% repellency at a dose of 0.2 μL/cm2. The above EO, together with the EO from the fruits of J. oxycedrus subsp. deltoides (JOX), were further analyzed by CPC using the biphasic system n-Heptane/ACN/BuOH in ratio 1.6/1.6/0.2 (v/v/v). The analysis of PPI essential oil resulted in the recovery of (−)-limonene, guaiol and simple mixtures of (−)-limonene/β-pheladrene, while the fractionation of JOX EO led to the recovery of β-myrcene, germacrene-D, and mixtures of α-pinene/β-pinene (ratio 70/30) and α-pinene/germacrene D (ratio 65/45). All isolated compounds and recovered mixtures were tested for their repellent activity. From them, (−)-limonene, guaiol, germacrene-D as well the mixtures of (−)-limonene/β-pheladrene presented significant repellent activity (>97% repellency) against Ae. albopictus. The present methodology could be a valuable tool in the effort to develop potent mosquito repellents which are environmentally friendly.

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