2017
DOI: 10.1038/ncomms14011
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Specialized interfaces of Smc5/6 control hinge stability and DNA association

Abstract: The Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin and Smc5/6 are involved in the organization of higher-order chromosome structure—which is essential for accurate chromosome duplication and segregation. Each complex is scaffolded by a specific SMC protein dimer (heterodimer in eukaryotes) held together via their hinge domains. Here we show that the Smc5/6-hinge, like those of cohesin and condensin, also forms a toroidal structure but with distinctive subunit interfaces absent from t… Show more

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Cited by 67 publications
(83 citation statements)
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“…It is worth noting that the ATPase activity in bacterial SMC complexes may regulate the dynamic association with DNA by altering the conformation of the coiled coil domains (Bürmann et al , ). As the Nse2 SUMO ligase docks to the coiled coil domain of Smc5, it is possible that the ATPase in the Smc5/6 complex might not only alter the juxtaposition of the coiled coils (Alt et al , ; Bürmann et al , ), but also promote the concomitant activation of Nse2 after entrapment of DNA inside the Smc5/6 ring.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…It is worth noting that the ATPase activity in bacterial SMC complexes may regulate the dynamic association with DNA by altering the conformation of the coiled coil domains (Bürmann et al , ). As the Nse2 SUMO ligase docks to the coiled coil domain of Smc5, it is possible that the ATPase in the Smc5/6 complex might not only alter the juxtaposition of the coiled coils (Alt et al , ; Bürmann et al , ), but also promote the concomitant activation of Nse2 after entrapment of DNA inside the Smc5/6 ring.…”
Section: Discussionmentioning
confidence: 99%
“…Once loaded onto chromatin, Smc5/6 participates in critical chromosome transactions during DNA replication and repair. The Smc5 and Smc6 subunits can bind strongly to DNA in vitro through several binding regions located in the hinge, the head and the arm regions (Roy & D'Amours, ; Roy et al , , ; Alt et al , ). In vivo , the affinity of the SMC core for DNA is regulated by the other six non‐SMC subunits (Nse proteins).…”
Section: Introductionmentioning
confidence: 99%
“…In addition, SMC5/6 contains several peripheral subunits. The budding yeast Nse5/6 heterodimer is a component of the Smc5/6 complex (essential for proliferation in budding yeast, but not in fission yeast) and has single-stranded (ss) DNA-binding activity [6]. The NSMCE1-NSMCE3-NSMCE4 sub-complex presents doublestranded (ds) DNA-binding activity, without preference for structured DNA [4].…”
Section: Introductionmentioning
confidence: 99%
“…DNA binding capabilities have been reported for hinge domains of several SMC complexes (Alt et al, 2017; Arumugam et al, 2003; Chiu et al, 2004; Griese and Hopfner, 2011; Griese et al, 2010; Hirano and Hirano, 2006; Soh et al, 2015; Uchiyama et al, 2015). In B. subtilis, the substitution of three consecutive lysine residues (K666, K667 and K668) at the hinge/coiled coil junction for glutamate strongly reduces DNA binding and DNA-stimulation of ATP hydrolysis in purified preparations of Smc (Hirano and Hirano, 2006).…”
Section: Resultsmentioning
confidence: 99%