Speciating<i>Campylobacter jejuni</i>and<i>Campylobacter coli</i>isolates from poultry and humans using six PCR-based assays

Burnett, Tracey A; Hornitzky, M.; Kuhnert, Peter; Djordjevic, Steven P.

doi:10.1111/j.1574-6968.2002.tb11436.x

Cited by 37 publications

(5 citation statements)

References 21 publications

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“…Genomic DNA was extracted from pure colonies using the Qiagen QIAamp ® PowerFecal ® kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions, followed by multiplex-PCR using genus-specific primers (C412F; C1228R), cj0414 gene primers (C1; C3), and ask gene primers (CC18F; CC519R) ( Table 3), as previously described with modification [73]. cj0414 is a conserved gene coding for a fragment of a putative oxidoreductase subunit gene (PID 6967888; Cj0414) of C. jejuni, while ask encodes aspartokinase, highly specific for C. coli [74][75][76]. The PCR mixture (25 µL) contained 12.5 µL of 2× Master Mix (Thermo Fisher Scientific, Seoul, Korea), 1 µL of each primer, 1.5 µL of DNA, and 7 µL of sterile deionized water.…”

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

Susceptibility of Campylobacter Strains to Selected Natural Products and Frontline Antibiotics

Gahamanyi

Song²,

Hyun³

et al. 2020

Antibiotics

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Campylobacter species have developed resistance to existing antibiotics. The development of alternative therapies is, therefore, a necessity. This study evaluates the susceptibility of Campylobacter strains to selected natural products (NPs) and frontline antibiotics. Two C. jejuni strains (ATCC® 33560TM and MT947450) and two C. coli strains (ATCC® 33559TM and MT947451) were used. The antimicrobial potential of the NPs, including plant extracts, essential oils, and pure phytochemicals, was evaluated by broth microdilution. The growth was measured by spectrophotometry and iodonitrotetrazolium chloride. Antibiotic resistance genes (tet(O) and gyrA) were characterized at the molecular level. The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) ranged from 25 to 1600 µg/mL. Cinnamon oil, (E)-Cinnamaldehyde, clove oil, eugenol, and baicalein had the lowest MIC and MBC values (25–100 µg/mL). MT947450 and MT947451 were sensitive to erythromycin and gentamicin but resistant to quinolones and tetracycline. Mutations in gyrA and tet(O) genes from resistant strains were confirmed by sequencing. The findings show that NPs are effective against drug-sensitive and drug-resistant Campylobacter strains. The resistance to antibiotics was confirmed at phenotypic and genotypic levels. This merits further studies to decipher the action mechanisms and synergistic activities of NPs.

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Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

Susceptibility of Campylobacter Strains to Selected Natural Products and Frontline Antibiotics

Gahamanyi

Song²,

Hyun³

et al. 2020

Antibiotics

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“…In contrast, no significant difference was reported between the results obtained with the culture and PCR methods for the detection of Campylobacter species in another study (20). The biochemical identification of C. jejuni isolated by the culture method mainly depends on the results of the hippurate hydrolysis test, which reacts positively for C. jejuni and is negative for other species of Campylobacter (21,22). However, some strains of C. jejuni react negatively to the hippurate hydrolysis test as a result of a failure in the transcription of the hipO gene (23).…”

Section: Discussionmentioning

confidence: 99%

Circulation of thermophilic Campylobacter in pigeons, turkeys, and humans at live bird markets in Egypt

Sayed

Ibrahim

Sobhy³

et al. 2023

Front. Vet. Sci.

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Live bird markets increase the risk of transmission of zoonotic diseases. Few studies have investigated the potential zoonotic transmission of Campylobacter in Egypt. Therefore, our study was carried out to investigate the presence of Campylobacter species, mainly Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli), in pigeons and turkeys sold at poultry shops. Furthermore, the study aimed to explore the potential occupational risk of Campylobacter infection, mainly among workers at poultry shops. Six hundred (n = 600) samples from various organs were obtained from pigeons and turkeys from live bird shops in the Giza and Asyut provinces in Egypt. Additionally, 100 stool samples were collected from persons working at poultry shops. Circulation of thermophilic Campylobacter in pigeons, turkeys, and humans was investigated based on culture and molecular methods. The rate of detection of Campylobacter species from the samples was significant when the culture method was used alone in comparison to when it was used in combination with mPCR. The prevalence rates of Campylobacter species detected by mPCR were 36% (C. jejuni 20%; C. coli 16%), 28% (C. jejuni 12%; C. coli16%), and 29% (C. jejuni 15%; C. coli 14%) in pigeons, turkeys, and workers, respectively. In pigeons, significant variations in the C. jejuni and C. coli occurrence rates were reported in terms of the intestinal content (15, 4%), liver (4, 13%), and skin (9, 7%), respectively. In turkeys, Campylobacter species were mostly detected in liver samples with a percentage of 19%, followed by the skin (12%), and the intestinal content (8%). In conclusion, Campylobacter species are circulating in poultry farms in Egypt and could represent a hazard for humans. It is recommended that biosecurity measures should be applied to mitigate the occurrence of Campylobacter in poultry farms. Moreover, there is an urgent need to transform live bird markets into chilled poultry markets.

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“…Furthermore, exploring the use of different genes that are not easily influenced by recombination and that can effectively differentiate between the closely related species C. coli and C. jejuni may potentially improve existing typing schemes (Klena et al, 2004). Additionally, based on the evaluation by Burnett et al (2002), 16S rRNA sequence-dependent assays may not be the best approach to differentiate these closely related species. Future work may also benefit from the use of an additional assay to confirm the identity of Campylobacter isolates prior to conducting MLST.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Campylobacter jejuni and Campylobacter coli isolates from chicken offal in Metro Manila, Philippines: Insights from virulence gene prevalence and multilocus sequence typing analysis

M.S.E.

2023

Trop Biomed

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Campylobacteriosis is a human infection primarily caused by Campylobacter jejuni and Campylobacter coli. Consumption of contaminated chicken and poultry products is the main mode of transmission. These bacteria possess virulence factors, including adhesins and toxins, which contribute to their pathogenesis. Moreover, their large genomes undergo frequent genetic recombination, resulting in a high degree of genetic diversity. However, limited information is available regarding the virulence and genotypic diversity profiles of these microorganisms in the Philippines. The objective of this study was to address this knowledge gap by characterizing Campylobacter isolates obtained from chicken offal sold in wet markets in Metro Manila, Philippines. Multilocus Sequence Typing (MLST) analysis was performed to determine the sequence types, resulting in the identification of 13 unique sequence types, including nine previously unreported ones, and three clonal complexes. Notably, the widespread sequence type ST-305 was found in samples from different markets. Furthermore, six isolates deposited in the Campylobacter PubMLST database were identified as C. coli based on allele profiles. Profiling using 10 selected virulence genes revealed that more than half of the isolates carried these genes. The most prevalent virulence gene was cadF (100%), followed by flaA (95%), racR, cdtA, cdtB, and cdtC (85%). The genes dnaJ and ceuE were also present in 75% of the isolates. Despite the limited sample size, the findings of this study reveal a significant level of genotypic diversity among the Campylobacter isolates. This diversity has important implications for source attribution studies and the identification of strains involved in campylobacteriosis outbreaks. Furthermore, the investigation of virulence factors associated with colonization and invasion of the avian gut can provide insights for the development of practical applications in Campylobacter control strategies. Understanding and addressing these factors are crucial steps toward mitigating the risk of Campylobacter infections and enhancing public health efforts.

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SpeciatingCampylobacter jejuniandCampylobacter coliisolates from poultry and humans using six PCR-based assays

Cited by 37 publications

References 21 publications

Susceptibility of Campylobacter Strains to Selected Natural Products and Frontline Antibiotics

Susceptibility of Campylobacter Strains to Selected Natural Products and Frontline Antibiotics

Circulation of thermophilic Campylobacter in pigeons, turkeys, and humans at live bird markets in Egypt

Characterization of Campylobacter jejuni and Campylobacter coli isolates from chicken offal in Metro Manila, Philippines: Insights from virulence gene prevalence and multilocus sequence typing analysis

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