Speciation analysis of mercury and lead in fish samples using liquid chromatography–inductively coupled plasma mass spectrometry

Chang, Lan-Fang; Jiang, Shiuh‐Jen; Sahayam, A.C.

doi:10.1016/j.chroma.2007.10.072

Cited by 61 publications

(27 citation statements)

References 27 publications

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“…It indicated that the efficiency of vapor generation may depend on the chemical form of mercury in the sample. Similar results were also reported in conventional chemical vapor generation [28] and photochemical vapor generation system. For example, the sensitivity of methylmercury is much higher than that of Hg 2+ in photochemical vapor generation [29].…”

Section: Analytical Figures Of Meritsupporting

confidence: 88%

Solution cathode glow discharge induced vapor generation of mercury and its application to mercury speciation by high performance liquid chromatography–atomic fluorescence spectrometry

Zhu

et al. 2011

Journal of Chromatography A

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Section: Analytical Figures Of Meritsupporting

confidence: 88%

Solution cathode glow discharge induced vapor generation of mercury and its application to mercury speciation by high performance liquid chromatography–atomic fluorescence spectrometry

Zhu

et al. 2011

Journal of Chromatography A

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“…Given these demethylation rates, it is possible that our assays were simply too short to measure low demethylation rates (a demethylation rate of 9%/day would be within the error of our MeHg analysis). We did not detect any production of Hg(II) i in the aqueous phase by high-pressure liquid chromatography (HPLC)-ICP-MS (12), which provides a direct and independent verification of slow demethylation in these experiments. In the washed-cell demethylation experiments reported here, we supplied 0.5 mM L-cysteine as the dominant MeHg ligand to match the association and methylation assays.…”

mentioning

confidence: 59%

Detailed Assessment of the Kinetics of Hg-Cell Association, Hg Methylation, and Methylmercury Degradation in Several Desulfovibrio Species

Graham

Bullock

Maizel

et al. 2012

Appl Environ Microbiol

125

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. We tested the hypothesis that differences in Hg(II) i sorption and/or uptake rates drive observed differences in methylation rates among Desulfovibrio species. Hg(II) i associated rapidly and with high affinity to both methylating and nonmethylating species. MeHg production by Hg-methylating strains was rapid, plateauing after ϳ3 h. All MeHg produced was rapidly exported. We also tested the idea that all Desulfovibrio species are capable of Hg(II) i methylation but that rapid demethylation masks its production, but we found this was not the case. Therefore, the underlying reason why MeHg production capability is not universal in the Desulfovibrio is not differences in Hg affinity for cells nor differences in the ability of strains to degrade MeHg. However, Hg methylation rates varied substantially between Hg-methylating Desulfovibrio species even in these controlled experiments and after normalization to cell density. Thus, biological differences may drive crossspecies differences in Hg methylation rates. As part of this study, Microbial mercury methylation is the main driver of risk associated with Hg pollution. Methylmercury production is an anaerobic process that occurs in saturated soils and wetlands (26,44,45,53), decaying periphyton mats (1, 14, 31), aquatic bottom sediments (16,27,33,36), and anaerobic bottom waters (56). Early investigations, prior to the advent of modern methylmercury (MeHg) analyses, reported a wide variety of aerobic and anaerobic Gram-positive and Gram-negative bacteria (30,49,55,58) and fungi (55) to be capable of MeHg production. However, subsequent studies with pure cultures have conclusively demonstrated a role only for sulfate-reducing bacteria (SRB) (4,8,11,13,20,23,38,50) and iron-reducing bacteria (FeRB; principally Geobacter spp.) (21, 37), all belonging to the Deltaproteobacteria. Many field studies, using selective microbial stimulants (1, 10, 26, 44, 57), inhibitors (1, 16, 24, 26, 59), and biogeochemical correlates (6,39,40,45), have buttressed the paradigm of SRB and FeRB as the dominant Hg methylators in natural aquatic systems (16,24,59), though recent studies have hypothesized that methanogens may be significant in some systems (31).Only a subset of SRB and FeRB are capable of Hg methylation (11,23,37,50), but why this is the case remains unclear. Early work by Choi and Bartha (13) suggested that Hg methylation was a "metabolic mistake" of SRB utilizing the acetyl coenzyme A (acetyl-CoA) pathway for carbon metabolism. Subsequent studies, however, indicated that Hg methylation capability is not restricted to SRB possessing the acetyl-CoA pathway (20). At present, it is not possible to conclusively identify the methyltransferase or methyl donor in SRB (or other Deltaproteobacteria) responsible for in vivo Hg methylation. Hg methylation occurs intracellularly (23), and significant effort has therefore been devoted to elucidating the mechanism(s) of Hg uptake by Hg-methylating bacteria.Passive diffusion of neutral HgS species has been hypothesized to control Hg uptak...

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“…In predatory marine fish, about 90% of 44 mercury is in the methylated form (methylmercury, MeHg) (WHO, 2008), while the 45 remainder consists of small or undetectable quantities of inorganic mercury [Hg(II)], 46 ethylmercury, and phenylmercury (Branco et al, 2007;Chang et al, 2007). The 47…”

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confidence: 99%

Mercury and methylmercury bioaccessibility in swordfish

Torres-Escribano

Vélez

Montoro

2010

Food Additives & Contaminants: Part A

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Speciation analysis of mercury and lead in fish samples using liquid chromatography–inductively coupled plasma mass spectrometry

Cited by 61 publications

References 27 publications

Solution cathode glow discharge induced vapor generation of mercury and its application to mercury speciation by high performance liquid chromatography–atomic fluorescence spectrometry

Solution cathode glow discharge induced vapor generation of mercury and its application to mercury speciation by high performance liquid chromatography–atomic fluorescence spectrometry

Detailed Assessment of the Kinetics of Hg-Cell Association, Hg Methylation, and Methylmercury Degradation in Several Desulfovibrio Species

Mercury and methylmercury bioaccessibility in swordfish

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