Speciation of arsenic in baby foods and the raw fish ingredients using liquid chromatography-hydride generation-atomic absorption spectrometry

Abstract

KeyWordsColumn liquid chromatography Atomic absorption spectrometry detection ArsenTc speciatTon Hydride generation Babyfood and fish SummaryThe specTatTon of arsenTc in dTfrerent baby foods and the raw fTsh Tngredients using the direct hybridTsation of liquid chromatography (LC) and hydride generation atomTc absorption spectrometry (HGAAS) Ts described. Good resolutTon of the species, arsenTc{lll), dimethylarsTnTc add (D/VCdk), monomethylarsonTc acid (MMAA) and arsenTc(V) Ts achieved usTng an anion-exchange column wth potassium phosphate as the mobile phase and gradTent elutTon.Arsenobetaine (AsB) is determTned by on-line oxidation usTng peroxydTsulphate and hydrTde generation. The arsenicals were extracted byan enzymatic digestion procedure based on the action of trypsin or pancreatin.Arsenobetaine was the only arsenicspecies detected.The reliabilityof the procedure was checked by analyzing the total arsenic content of the samples by electro thermal atomic absorption spectrometry wth microwave oven digestion and by analyzing a certified reference material. The arsenic content in the baby foods comes from the raw fish in gredients and is highestwhen plaice is used.

Feasibility of ion-exchange solid phase extraction inductively coupled plasma mass spectrometry for discrimination between inorganic As(III) and As(V) in phosphate-rich in vitro bioaccessible fractions of Ayurvedic formulations

“…The authors also pointed out at the lack of an adequate procedure for bioaccessibility studies. However, pancreatin is one of the most popular compounds for the simulation of duodenal juice [30,[35][36][37][38]. Thus, about 19% of total As represented the readily soluble fraction that together with the carbonatic one correlated with the bioaccessible percent.…”

Achieving 100% Efficient Postcolumn Hydride Generation for As Speciation Analysis by Atomic Fluorescence Spectrometry

“…Mobile phase for ion pair chromatography was 4.7 mmol L –1 tetrabutylammonium hydroxide (Fluka, Germany) and 2 mmol L –1 malonic acid (Sigma-Aldrich, Germany) in 4% methanol (Sigma-Aldrich, Germany); pH was adjusted to 5.85 by KOH . Mobile phase for anion exchange chromatography was 20 mmol L –1 phosphate buffer (from KH 2 PO 4 (Merck, Germany) and K 2 HPO 4 (Xenon Lodz, Poland)), pH adjusted to 6.00 . Solid NaOH (Lach-Ner, Czech Republic) in the form of 3 mm pearls was used as a filling of a NaOH dryer …”

Atomic spectrometry update. Advances in atomic emission, absorption and fluorescence spectrometry and related techniques

“…Heinrich-Ramm et al 271 investigated the influence of controlled consumption of marine fish on the urinary excretion of As(III), As(V), dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA). Vinas et al 272 speciated As baby foods and the raw fish ingredients. Alauddin et al 273 speciated arsenic metabolite intermediates in the urine from patients in Hajiganj, a seriously arsenic-affected area in Bangladesh.…”

“…Some As species do not readily form a hydride so, to detect them using this method, an on-line process is necessary to convert such species into a reducible form. An example has been published by Vinas and co-workers, 272 who speciated As in baby foods and raw fish ingredients. After enzymatic extraction using either trypsin or pancreatin, several species including As(III) and As(V), DMA, MMA and arsenobetaine (AsB) were separated using an anion exchange column and phosphate as a mobile phase.…”