Speciation of arsenic in mussels by the coupled system liquid chromatography—UV irradiation—hydride generation-inductively coupled plasma mass spectrometry

Dagnac, Thierry; Padró, A.; Rubio, R.; Rauret, G.

doi:10.1016/s0039-9140(98)00093-9

Cited by 62 publications

(26 citation statements)

References 19 publications

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“…Low-power microwave digestion has been shown to be mild and fast for the extraction of arsenic species from seafood products. 16,19,22,37 In the preliminary studies, water and 50% methanol were investigated separately for arsenic extraction in marine algae. The aqueous methanol medium showed a better extractability (>75%) than the water medium (<65%).…”

Section: Arsenic Extractionmentioning

confidence: 99%

Ion chromatography–inductively coupled plasma mass spectrometry determination of arsenic species in marine samples

Karthikeyan

Hirata

2004

Applied Organom Chemis

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Arsenic speciation analysis in marine samples was performed using ion chromatography (IC) with inductively coupled plasma mass spectrometry (ICP-MS) detection. The separation of eight arsenic species, viz. arsenite, monomethyl arsonic acid, dimethylarsinic acid, arsenate, arsenobetaine, tetramethylarsine oxide, arsenocholine and tetramethylarsonium ion was achieved on a Dionex AS4A (weaker anion exchange column) by using a nitric acid pH gradient eluent (pH 3.3 to 1.3). The entire separation was accomplished in 12 min. The detection limits for the eight arsenic species by IC-ICP-MS were in the range 0.03-1.6 µg l −1 , based on 3σ of the blank response (n = 6). The repeatability and day-to-day reproducibility were calculated to be less than 10% (residual standard deviation) for all eight species. The method was validated by analyzing a certified reference material (DORM-2, dogfish muscle) and then successfully applied to several marine samples, e.g. oyster, fish muscle, shrimp and marine algae. The low power microwave digestion was employed for the extraction of arsenic from seafood products.

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Section: Arsenic Extractionmentioning

confidence: 99%

Ion chromatography–inductively coupled plasma mass spectrometry determination of arsenic species in marine samples

Karthikeyan

Hirata

2004

Applied Organom Chemis

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“…The procedures for analyzing As (III), As(V), MMA and DMA followed closely those in our earlier work (Huang et al 2003). The AsB assay method was modified from Alberti et al (1995), Dagnac et al (1999), andGeiszinger et al (1998). A 200-μ aliquot of the fish muscle extract was injected into HPLC (Hitachi 7100, Naka, Japan) equipped with a cation column (matachem, necleosil, 5 μm, 250× 4.6 mm).…”

Section: Sample Collection and Analysismentioning

confidence: 99%

Spatiotemporal distribution of arsineic species of oysters (Crassostrea gigas) in the coastal area of southwestern Taiwan

Liu

Huang

Hsueh

et al. 2007

Environ Monit Assess

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This study investigated total arsenic (As) and As species contents of oysters (Crassostrea gigas) in different production areas, seasons and sea locations on the southwestern coast of Taiwan. Analytical results indicate that contents of total As, arsenite, arsenate, dimethylarsinic acid, monomethylarsonic acid and arsenobetaine in oysters are 9.90+/-3.68, 0.091+/-0.104, 0.033+/-0.038, 0.529+/-0.284, 0.037+/-0.046 and 3.94+/-1.33 mg/g (dry wt), respectively. A ratio of inorganic As concentrations to total As concentrations is 1.26%. Total As contents of oysters cultured in the outer sea are statistically significantly lower than those of oysters cultured in the inner sea. The total As contents of oysters is the highest in Putai, where the blackfoot disease prevails. The low As contents in oysters is attributed to the low temperature in winter, which slows the metabolism of oysters. A maximum value is 33.37 microg/g (dry) in Putai in spring, because a considerable amount of aquacultural waste water with high As contents is discharged into adjacent drainage channels and rivers there during that season.

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“…Most of them make use of hyphenated systems that link a separation technique and a detector. For arsenic speciation studies on water and biological samples, inductively coupled plasma–mass spectrometry (ICP‐MS)‐based detection methods (Kubachka et al ., ; Qin et al ., ; Conklin et al ., ; Dagnac et al ., ) with low detection limits (0.5 µg L −1 ) have a clear advantage over the more conventional ATOMIC ABSORPTION SPECTROMETRY (AAS‐) and ATOMIC FLOURESCENCE SPECTROMETRY (AFS‐)based detection methods with higher detection limits (50 µg L −1 ) (Gailer and Irgolic, ; Terasahde et al ., ). Despite the high sensitivity, the use of HPLC‐ICP‐MS for arsenic speciation studies still faces issues, which may particularly be important when analyzing As speciation in gastrointestinal environment.…”

Section: Introductionmentioning

confidence: 99%

HPLC‐ICP‐MS method development to monitor arsenic speciation changes by human gut microbiota

Alava

Tack

Laing

et al. 2011

Biomedical Chromatography

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Inorganic arsenic (iAs) has been classified as a type 1 carcinogen and has also been linked to several noncancerous health effects. Prior to 1995, the As(V) methylation pathway was generally considered to be a detoxification pathway, but cellular and animal studies involving MMA(III) (mono metyl arsonous acid) and DMA(III) (dimethyl arsinous acid) have indicated that their toxicities meet or exceed that of iAs, suggesting an activation process. In addition, thiolated arsenic metabolites were observed in urine after oral exposure of inorganic arsenic in some studies, for which the toxicological profile was not yet fully characterized in human cells. Studies have revealed that microorganisms from the gut environment are important contributors to arsenic speciation changes. This presystemic metabolism necessitates the development of protocols that enable the detection of not only inorganic arsenic species, but also pentavalent and trivalent methylated, thiolated arsenicals in a gastrointestinal environment. We aim to study the biotransformation of arsenic (As) using a Simulator of the Human Intestinal Microbial Ecosystem (SHIME). To be able to analyze the arsenicals resulting from biotransformation reactions occurring in this system, a method using liquid chromatography hyphenated to an inductively coupled plasma mass spectrometer (HPLC-ICP-MS) was developed. A Hamilton PRP-X100 anion exchange column was used. The method allowed separation, identification and quantification of As(III) (arsenite), As(V) (arsenate), DMA(V) (dimethylarsinicacid), MMA(V) (monomethylarsonicacid) and MMMTA (monomethylmonothioarsenate). Attempts to optimize the same method for also separating MMA(III) and DMA(III) did not succeed. These compounds could be successfully separated using a method based on the use of a Zorbax C₁₈ column. The properties of the column, buffer strength, pH and polar nature of mobile phase were monitored and changed to optimize the developed methods. Linearity, sensitivity, precision, accuracy and resolution of both methods were checked. The combination of the two methods allowed successful quantification of arsenic species in suspensions sampled in vitro from the SHIME reactor or in vivo from the human colon and feces.

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Speciation of arsenic in mussels by the coupled system liquid chromatography—UV irradiation—hydride generation-inductively coupled plasma mass spectrometry

Cited by 62 publications

References 19 publications

Ion chromatography–inductively coupled plasma mass spectrometry determination of arsenic species in marine samples

Ion chromatography–inductively coupled plasma mass spectrometry determination of arsenic species in marine samples

Spatiotemporal distribution of arsineic species of oysters (Crassostrea gigas) in the coastal area of southwestern Taiwan

HPLC‐ICP‐MS method development to monitor arsenic speciation changes by human gut microbiota

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