Speciation of Candida using HiCrome Candida Differential Agar

Kaup, Soumya; Sankarankutty, Jaya; Balasubrahmanya, H.V.; Kulkarni, Suma; Nirmala, M. Joyce

doi:10.20546/ijcmas.2016.507.027

Cited by 8 publications

(6 citation statements)

References 7 publications

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“…In this study, Candida was isolated most commonly in the age group of 0<1year (53.7%), followed by 41-60years (31.5%), 21-40years(7.5%) and >60years (7.4%) which is similar to the study conducted by L. Sumitra Devi et al 15 in which Candida was mostly isolated in the age group of 0-<1year old (60.9%), followed by 21-40 years (12.5%), >60 years (10.9%),1-20 years (7.8%) and 41-60 years (7.8%). This study does not correlate with the study don by Soumya Kaup et al 16 in which majority of Candida isolates from patients aged between 61-70 years followed by 41-50 & 51-60 years.…”

Section: Discussioncontrasting

confidence: 99%

Speciation of candida species isolated in clinical samples in a tertiary health care centre in Northern India

Kaur

Rawat²,

Sharma³

et al. 2021

IJMMTD

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The purpose of this study is to isolate, identify and specification of various the Candida species from various clinical samples in a tertiary care hospital, and to characterize various the isolated Candida species. A study was conducted on people of different age groups from January 2019 to December 2019. Candida species isolated from different patients by using Potassium Hydroxide mount and processed by BacTalert 3D (Biomerieux) automated blood culture system. Further culture identification of Candida species were done on Sabouraud dextrose agar (SDA). Speciation of Candida was done using Germ tube test, CHROM agar Candida Medium, Cornmeal agar, Sugar Fermentation test and Sugar Assimilation test.In our study was the most common species isolated, among non albicans Candida i.e. 21 (38.9%); 19(35.2%) of was the most common followed by 9(16.7%) ofand 5(9.3%) of . Maximum number of Candida isolates were obtained from NICU i.e. 27(50.0%) followed by 11 from Med (20.3%), 7 from E/W (13.0%), 2 from BICU (3.7%), 2 from Skin (3.7%), 1 from PICU (1.9%), and 1 from R/R (1.9%).Our study showed that is the most common isolates species. Among , was found to be the most common isolate followed by . Children less than 1 year are most affected with maximum number of Candida species were obtained from NICU department. HiChrom Candida is proven to be more useful as differential agar.

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Section: Discussioncontrasting

confidence: 99%

Speciation of candida species isolated in clinical samples in a tertiary health care centre in Northern India

Kaur

Rawat²,

Sharma³

et al. 2021

IJMMTD

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“…Procedure; 5.8 g of the NA was dissolved into 200 ml of distilled water; this is calculated as follows: 28 * 2001000=5.6 𝑔 The mixture was heated while stirred with a magnetic stirrer to be dissolved completely and the formed media was then sterilized using an autoclave at 15 psi and the temperature of 121°C for about 15 minutes. This was allowed to cool down without solidifying then the media was carefully dispensed into sterile petri dishes as described by Soumya and Jaya (2016). Isolation, and Identification of bacteria from leachate samples: Isolation of Bacteria from Leachate Samples: EMB culture media was prepared and the leachate samples collected were inoculated using swab stick in a sterilized environment, the standard streak method was adopted and the media was incubated at 37 °C for a period of 24hours.…”

Section: Microbial Analyses Of Leachates: Media Preparationmentioning

confidence: 99%

Some Physical Properties and Bacteriological Evaluation of Raw Landfill Leachate from Gosa Landfill in the Federal Capital Territory, Abuja, Nigeria

Nasir¹,

Mohammed²,

Mangse³

2023

jasem

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The physical and bacteriological evaluation of raw landfill leachate from Gosa Landfill in FCT, Abuja, Nigeria was carried out in this study using standard procedures. The probable bacteria identified from the biochemical tests of isolated samples were Streptococcus pneumoniae with Pseudomonas aeruginosa and Staphylococcus aureus. However, molecular characterization involving sequencing and BLAST further confirmed the bacteria present. This study concludes that the bio filter produced from Coco peat and Ash affects both the physicochemical properties and bacteriological flora of raw landfill leachate through degradation.

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“…Gram stain was applied to the colonies from the SDA plate to confirm Candida growth [19]. all isolated colonies were inoculated on HiCrome™ Candida Differential agar (HiMedia, India) and incubated at 37⁰ C for 48 hours [20].…”

Section: Samples Culturing and Isolating Candida Speciesmentioning

confidence: 99%

Molecular identification of Candida spp. isolated from women withvulvovaginal candidiasis in Koya District, Iraq

Mohammed,

Zrary

2023

JZS

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Vulvovaginal candidiasis (VVC) is the second most common vaginal infection among women of reproductive age. It is an opportunistic mucosal infection. Candida species causes VVC, which affects millions of women annually. The objective of this study was to isolate and identify the species of Candida from married women who had VVC attending Shahid Doctor Khalid Hospital & Haji Qader Health Centre in Koya, Iraq using traditional and molecular methods. This research was carried out, from December 2021 to May 2022; 150 vaginal swabs from married patients with symptoms of VVC were collected. Swabs were microscopically examined, grown on Sabouraud Dextrose Agar (SDA), and HiCrome™ Candida Differential agar and Germ Tube Test were used to identify Candida isolates. Next, the results were validated using the molecular method called polymerase chain reaction (PCR) to amplify and detect the ITS region in this study. The chitin synthase gene (CHS1) confirmed all C. albicans isolates. Out of the150 vaginal swabs gathered, 84 (56%) cases were positive for vaginal candidiasis. Ages26-30 were found to have the most significant proportion of positive patients, and Women who were pregnant had a more positive culture than women who were not. Identifying six different species of Candida was accomplished through the application of both traditional and molecular techniques in the study; they are C. albicans (88.1%), C. glabrata (4.8%), C. kefyr (2.4%), C. krusei (2.4%), C. parapsilosis (1.2%), and C. tropicalis (1.2%). Finally, we concluded that VVC was shown to be prevalent in Koya city, with the predominant species as C. albicans among all other species.

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Speciation of Candida using HiCrome Candida Differential Agar

Cited by 8 publications

References 7 publications

Speciation of candida species isolated in clinical samples in a tertiary health care centre in Northern India

Speciation of candida species isolated in clinical samples in a tertiary health care centre in Northern India

Some Physical Properties and Bacteriological Evaluation of Raw Landfill Leachate from Gosa Landfill in the Federal Capital Territory, Abuja, Nigeria

Molecular identification of Candida spp. isolated from women withvulvovaginal candidiasis in Koya District, Iraq

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